نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I

نویسندگان

1 .دانشجوی دکتری گروه علوم زیستی ورزش، دانشکده علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران

2 دانشیار گروه علوم زیستی ورزش، دانشکده علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران

3 . استادیار گروه علوم زیستی ورزش، دانشکدۀ علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران،

4 دانشیار گروه فارماکولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

چکیده

انقباض برون‌گرا حین فعالیت‌های ورزشی موجب آسیب عضلانی و سارکولما می‌شود. میتسوگمین53 و دیسفرلین، عمده پروتئین‌های ترمیم سارکولما هستند. بنابراین هدف از تحقیق حاضر، بررسی اثر آسیب عضلانی ناشی از فعالیت برون‌گرا در زمان‌های مختلف بر میتسوگمین53 و دیسفرلین در عضلۀ اسکلتی است. 50 سر موش نر صحرایی بعد از آشناسازی با محیط و نوار گردان به پنج گروه کنترل و پنج گروه فعالیت برون‌گرا تقسیم شدند. عضلۀ نعلی حیوانات نیم، 24، 48، 72 و 168 ساعت (یک هفته) بعد از انجام فعالیت برون‌گرا (90 دقیقه دویدن تناوبی با سرعت 16 متر در دقیقه و شیب 16- درجه) در شرایط استریل‌شده خارج شد. برای اندازه‌گیری تعداد سلول‌های التهابی و استخراج پروتئین‌های میتسوگمین 53 و دیسفرلین از روش‌های رنگ‌آمیزی هماتوکسیلین-ائوزین و وسترن بلات استفاده شد. از آزمون تحلیل واریانس دوطرفه و تی-مستقل برای مقایسه میانگین‌ها با سطح معناداری 05/0≥p استفاده شد. تعداد سلول‌های التهابی، پروتئین‌های میتسوگمین53 و دیسفرلین در زمان‌های نیم، 24، 48، 72 و 168 ساعت (یک هفته) بعد از فعالیت برون‌گرا در گروه فعالیت برون‌گرا به‌طور معناداری بیشتر از گروه کنترل بود (001/0>P). افزایش سلول‌های التهابی آسیب عضلانی ناشی از فعالیت ورزشی را نشان می‌دهد. نتایج تحقیق حاضر نشان داد که افزایش سلول‌های التهابی، آسیب عضلانی ناشی از فعالیت ورزشی را نشان می‌دهد. آسیب سارکولما موجب فعال شدن مکانیسم‌های ترمیم غشا می‌شود. افزایش پروتئین‌های میتسوگمین53 و دیسفرلین موجب افزایش ترمیم و ثبات سارکولما  می‌شود که متعاقب آن آسیب عضلانی و سلول‌های التهابی کاهش می‌یابد.    

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Eccentric Exercise Induced Muscle Damage at Different Times on Mitsogomin53 and Dysferlin Levels in Rat Soleus Muscle

نویسندگان [English]

  • Tohid Hemmatzade Beddovli 1
  • Maryam Nourshahi 2
  • Raana Fayyaz Milani 3
  • Siavash Parvardeh 4

1 PhD Student, Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran

2 Associate Professor, Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran

3 Assistant Professor, Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran

4 . Associate Professor, Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

چکیده [English]

Eccentric contraction during exercise causes muscle and sarcolemma damage. Mitsogomin53 and Dysferlin are the major sarcolemma repair proteins. Therefore, the aim of the present study was to investigate the effect of eccentric exercise-induced muscle damage at different times on skeletal muscle Mitsogomin53 and Dysferlin. Fifty male Wistar rats, after familiarization with the environment and the treadmill were divided into five control groups and five eccentric exercise groups. Soleus muscle in sterile conditions disassociate half, 24, 48, 72 and 168 hours (one week) after eccentric exercise (90 min interval running with 16 meter/min and -16 degree slope). Hematoxylin-eosin and western blotting methods were used to measure the number of inflammatory cells and extract the proteins of Mitsogomin53 and Dysferlin. Two-way ANOVA and independent t-test were used to compare the means with the significance level of p≤0.05. The number of inflammatory cells, Mitsogomin53 and Dysferlin proteins at the half, 24, 48, 72 and 168 hours (one week) after eccentric exercise in the exercise group were significantly higher than the control group (p

کلیدواژه‌ها [English]

  • EIMD
  • Dysferlin
  • inflammatory cells
  • eccentric exercise
  • Mitsogomin53
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