نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزشی دانشکدۀ علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران
2 استاد دانشکدة علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران
3 . استاد گروه فیزیولوژی، دانشکدة پزشکی دانشگاه علوم پزشکی شهید صدوقی، یزد، ایران
4 دانشیار گروه فیزیولوژی، دانشکدة پزشکی دانشگاه علوم پزشکی شهید صدوقی، یزد، ایران
چکیده
دیابت نوع دو نوعی بیماری مزمن متابولیکی است که با کلسیفیکاسوین عروقی و اختلال در میزان کربوهیدراتها، لیپیدها و کمبود یا کاهش حساسیت به انسولین مشخص میشود. از طرفی به‌نظر میرسد نقش فعالیت ورزشی در کاهش میزان کلسیفیکاسوین عروقی مورد توجه است. در تحقیق حاضر به این مسئله پرداخته شد که آیا ۸ هفته تمرین تناوبی شدید (HIIT)به کاهش کلسیفیکاسیون عروقی و بهبود نیمرخ لیپیدی در موش‌های مبتلا به دیابت نوع دو منجر میشود. پژوهش حاضر از نوع بنیادی و روش آن تجربی است. در این مطالعه۴۰ سر رت نر نژاد ویستار پس از آشناسازی با محیط آزمایشگاه به‌طور تصادفی به چهار گروه دیابت (۱۰T2D, n=) دیابت- ورزش (۱۰EX-T2D, n=)، ورزش- کنترل (۱۰EX,CON n=) و کنترل (۱۰CON, n=) تقسیم شدند. طی 5 هفته مراحل القای دیابت به حیوانات در گروه دیابت انجام گرفت. تمرینات اینتروال ۳ جلسه در هفته به مدت ۸ هفته با ۱۰۰ درصد حداکثر سرعت دویدن روی نوار گردان اجرا شد. برای بررسی بیان ژن RUNX2 از روش Real time PCR , و روش کالیمتریک به‌منظور ارزیابی انسولین و گلوکز و شاخصهای نیمرخ لیپیدی استفاده شد. حیوانات در گروه T2D در مقایسه با گروه کنترل افزایش در میزان گلوکز، تری‌گلیسیرید، کلسترول (۰۰۱/۰ P≤) و LDL (۰۵/۰ P≤) همچنین کاهش انسولین و HDL (۰۵/۰ P≤) را نشان دادند. در گروه ورزش بیان RUNX2 نسبت به گروه کنترل کاهش معنا‌داری داشت (۰۵/۰ P≤). همچنین میزان تری گلسیرید و کلسترول در گروه EX-T2D در مقایسه با گروه دیابت کمتر بود (۰۵/۰ P≤) . نتایج تحقیق حاضر نشان داد تمرین تناوبی شدید منجر به کاهش کلسیفیکاسیون عروقی و بهبود شاخصهای نیمرخ لیپیدی در موش‌های مبتلا به دیابت نوع دوم شد. با این حال مطالعات بیشتری مورد نیاز است
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of 8 weeks of high-intensity interval training on vascular calcification index in mice with type 2 diabetes
نویسندگان [English]
- Elham Khani Sanij 1
- Khosro Ebrahim 2
- Ebrahim Rezvani 3
- Hossein Azizian 4
1 Student, Faculty of Sport Sciences and Health. Shahid Beheshti University, Tehran, Iran
2 Professor, Faculty of Sport Sciences and Health. Shahid Beheshti University, Tehran, Iran
3 Professor, Department of Physiology, School of Medicine Shahid Sadoughi University of Medical Sciences. Yazd. Iran
4 Associated Professor, Department of Physiology, School of Medicine Shahid Sadoughi University of Medical Sciences. Yazd. Iran
چکیده [English]
Diabetes mellitus (DM) is a chronic metabolic disease characterized by vascular classification and impaired carbohydrates, lipids, and lack of insulin secretion or decreased sensitivity to insulin metabolic effects. on the other hand, it seems the effect of exercise on vascular classification is an important issue. In the present study, we evaluated whether 8 weeks of high-intensity interval training (HIIT) decrease vascular calcification and improvement lipid profile in rats. Main Methods: 40 Male Wistar rats were randomly divided into diabetic (T2D), exercise-diabetic (EX-T2D), exercise (EX-CON), and control (CON) groups. After 5 weeks, diabetes was induced in all the T2D and the EX-T2D group. The EX-T2D group trained for 8 weeks. Real-time PCR and colorimetric were performed to investigate the expression of RUNX2 and lipid profile Key Findings: Rat in the T2D group had a significant increase in glucose, triglyceride, cholesterol (p≤0.001), and LDL(p≤0.05) as well as deceased in insulin and HDL (p≤0.05). compared to the control group. In exercise, groups of rats had a significant decrease in RUNX2 expression compared to the control group (p≤0.05). in addition, Triglyceride and cholesterol levels were lower in the EX-T2D group compared to the diabetes group (P≤0.05). Significance: Our data demonstrate that HIIT decreased vascular calcification and improved lipid profile in a mouse model of diabetes. However, further research is required to examine potential clinical relevance.
کلیدواژه‌ها [English]
- Vascular calcification
- High intensity interval training
- RUNX2
- Insulin
Copyright © 2021
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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