مقایسۀ اثر چهار برنامۀ تمرینی به مدت هشت هفته بر بیان برخی آنزیم‌های آنتی‌اکسیدانی بافت قلب موش‌های صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران

2 کارشناس ارشد، گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور،تهران، ایران

3 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور،تهران، ایران

4 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

با توجه به اهمیت پیشگیری از عوامل بروز بیماری‌های قلبی و نیز اطلاعات متناقض در خصوص تأثیر تمرین‌های ورزشی بر بیان ژن‌های گلوتاتیون پراکسیداز (GPX) و کاتالاز (CAT)، هدف مطالعة حاضر مقایسة اثر 4 هفته برنامة تمرینی به مدت 8 هفته بر بیان GPX و CAT در بافت قلب موش‌های صحرایی بود. بدین‌منظور 40 سر موش صحرایی نر ویستار 8 هفته‌ای به‌طور تصادفی به 5 گروه شامل گروه‌های کنترل، تمرین هوازی با شدت متوسط، تمرین هوازی شدید (HIT)، تمرین هوازی تناوبی شدید (HIIT) و تمرین شنا با شدت متوسط (MIST) تقسیم شدند. برای تجزیه‌وتحلیل داده‌ها از تحلیل واریانس یکطرفه و توکی استفاده شد. نتایج نشان داد، افزایش معناداری در بیان ژن‌های GPX وCAT متعاقب هر چهار پروتکل ورزشی در مقایسه با گروه کنترل (001/0P=) و همچنین افزایش معناداری در بیان GPX میان گروه‌های HIIT و HIT نسبت به گروه‌های MIT و MIST (001/0P=) مشاهده شد. همچنین افزایش معناداری در بیان CAT، میان گروه MIST نسبت به گروه‌های MIT و HIIT (به‌ترتیب 001/0P= و 030/0P=) و بین گروه HIT نسبت به گروه‌های MIT و HIIT مشاهده شد (به‌ترتیب 001/0P= و 041/0P=). ضمن اینکه بین گروه‌های MIT و HIIT نیز اختلاف معناداری وجود داشت (001/0P=). هر چهار شیوة تمرینی با بهبود سیستم آنتی‌اکسیدانی، تغییرات مطلوبی در بافت قلب رت‌های ویستار ایجاد کرد. به‌نظر می‌رسد تمرینات HIT، HIIT و MIST با ایجاد سازگاری مفید در سیستم آنتی‌اکسیدانی، بدن را در مقابل فشار اکسایشی مقاوم‌تر ساخته و تأثیر مطلوب‌تری بر بافت قلبی داشته باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparison of four exercise training protocol for eight weeks on expression of some antioxidant enzymes in heart tissue of rats

نویسندگان [English]

  • mohammadreza asad 1
  • Zeynab Torabi 2
  • Ali Barzegari 3
  • Hassan Amouzad Mahdirejei 4
1 Associate Professor, Department of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran
2 . MSc, Department of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran
3 . Asistant Professor, Department of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran
4 Ph.D. Student of Exercise Physiology, Department of Exercise Physiology, Tehran Central Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Considering the importance of preventing heart disease and conflicting information about the effect of exercise training on glutathione peroxidase and catalase gene expression, the aim of this study was to investigate the effect offour training methods on Glutathione peroxidase and catalase expression in heart tissue of rats. For this purpose, 40 male wistar rats with age of 8-weeks were randomly divided into 5 groups including control group, moderate intensity training, high intensity training, high intensity interval training and moderate-intensity swimming training. To analyze of the data, one-way ANOVA and Tukey was used. The results showed that there was a significant increase in the Glutathione peroxidase and catalase expression as a result of all four exercise training program compared to the control group (P=0.001), Also, a significant increase in GPX expression was observed between HIIT and HIT groups compared to MIT and MIST groups (P=0.001). On the other hand, there was a significant increase in CAT expression between MIST group compared to MIT and HIIT groups (P=0.001 and P=0.030, respectively) and between HIT group compared to MIT and HIIT groups. There was also a significant difference between MIT and HIIT groups (P=0.001). All four training methods were able to improve the antioxidant system, causing favorable changes in the heart tissue of Wistar rats. It seems that HIT، HIIT & MIST by creating a beneficial adaptation in the antioxidant system, has made the body more resistant to the oxidative stress and has a more favorable effect on the heart tissue.

کلیدواژه‌ها [English]

  • Catalase
  • Exercise Training
  • Glutathione peroxidase
  • left ventricle
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