بررسی تغییرات محتوای پروتئینCas3، فیبرونکتین و Col4a1 در پی دو شیوۀ تمرین هوازی و اینتروال همراه با مصرف مکمل آستاگزانتین در بافت قلب رت‌های دیابتی نوع2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموختۀ دکتری فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی، دانشگاه ارومیه، ارومیه، ایران

2 استادیار فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی، دانشگاه ارومیه، ارومیه، ایران

3 دانشیار فیزیولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران

چکیده

دیابت به‌عنوان نوعی اختلال متابولیک مزمن با شیوع گسترده، اهمیت تحقیقات مداوم و نیاز به روش‌های نوین را برای پیشگیری و درمان این بیماری همه‌گیر برجسته می‌کند. هدف از تحقیق حاضر بررسی تغییرات فیبروزی و آپوپتوزی بافت قلبی در رت‌های دیابتی در پی اجرای دو شیوۀ تمرینی هوازی و اینتروال با مصرف مکمل آستاگزانتین بود. 35 سر موش صحرایی نر پس از القای دیابت به‌طور تصادفی در 7گروه شامل دیابتی کنترل، دیابتی شم، دیابت+ هوازی+ مکمل، دیابت + اینترول+ مکمل، دیابت+اینتروال، دیابت+ هوازی، دیابت+ مکمل قرار گرفتند.تمرینات اینتروال به مدت 8 هفته با 5 جلسه در هفته با شدت 80 درصد Vo2max و تمرینات هوازی با شدت 65 تا 75 درصد Vo2max روی نوار گردان اجرا شد. محتوای پروتئین Cas3، COL4a1 و فیبرونکتین با تکنیک وسترن بلات اندازه‌گیری شد. محتوای پروتئینCOL4a1  در هر دو گروه تمرینی و ترکیبی و گروه مکمل کاهش معناداری نسبت به گروه کنترل نشان داد. محتوای پروتئین Cas3 و فیبرونکتین گروه‌های ترکیبی و هوازی نیز نسبت به گروه کنترل کاهش معناداری داشت، اما در مقایسۀ محتوای این پروتئین‌ها بین گروه‌های مکمل و اینتروال نسبت به گروه کنترل اختلاف معناداری وجود نداشت. به‌نظر می‌رسد تأثیرات هم‌افزای تمرینات ورزشی با شدت‌های مختلف همراه با مکمل‌سازی آستاگزانتین از روند صعودی آپوپتوز و فیبروز در بافت قلبی رت‌های دیابتی و عوارض متعاقب آن همچون کاردیومیوپاتی دیابتی پیشگیری می‌کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of changes in Cas3, fibronectin and Col4a1 protein content following aerobic and interval training with astaxanthin supplementation in heart tissue of type 2 diabetic rats

نویسندگان [English]

  • nasrin ebadi 1
  • mohammadreza zolfaghary 2
  • Firouz Ghaderi Pakdel 3
1 PhD of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran
2 Assistant Professor of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran
3 Associate Professor of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
چکیده [English]

Diabetes, as a chronic metabolic disorder with a high prevalence, highlights the importance of ongoing research and the need for new methods to prevent and treat this epidemic disease.The aim of this study was to evaluate the changes in fibrosis and apoptosis of heart tissue in diabetic rats following two aerobic and interval training methods with astaxanthin supplementation. 35 Wistar male rats were randomly divided into7groups after induction of diabetes, including diabetic control, diabetes sham, diabetes+aerobic exercise+supplement. Diabetes+ intervalexercise + supplement, diabetes + interval, diabetes + aerobic, diabetes + supplement were included. Interval training for 8 weeks with 5 sessions per week with an intensity of 80% vo2max and aerobic training with an intensity of about 65 to 75% Vo2max was performed on the treadmill.The protein content of Cas3, COL4a1 and fibronectin were measured by Western blotting. COL4a1 protein content in both training and combination groups and supplement group showed a significant decrease with the control group. Also, the content of Cas3 and fibronectin protein in the combined and aerobic groups was significantly reduced compared to the control group, but there was no significant difference in the content of these proteins between the supplement and interval groups compared to the control group. It seems that the synergistic effects of exercise with different intensities along with astaxanthin supplementation prevent the upward trend of apoptosis and fibrosis in the heart tissue of diabetic rats and its subsequent complications such as diabetic cardiomyopathy.

کلیدواژه‌ها [English]

  • Astaxanthin
  • exercise
  • diabet
  • rat
  • Diabetic cardiomyopathy
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