نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دانشیار گروه فیزیولوژی ورزش دانشکدة تربیت بدنی دانشگاه شهید بهشتی، تهران، ایران
2 کارشناسی ارشد فیزیولوژی ورزش، دانشگاه شهید بهشتی، تهران، ایران
3 دانشیار مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران
4 دانشجوی دکتری، دانشکده علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران
چکیده
شیوع چاقی و بیماری‌های ناشی از آن در سراسر دنیا به معضلی بهداشتی تبدیل شده است. تغییر فنوتیپ بافت چربی سفید به قهوه‌ای در پی فعالیت ورزشی امروزه به‌عنوان یکی از راهکارهای پیشگیری چاقی مورد بررسی است. هدف از این تحقیق مقایسة تأثیر 8 هفته تمرین تداومی و تناوبی با شدت بالا (HIIT) بر میزان UCP1 بافت چربی احشایی و زیرپوستی موش‌های صحرایی چاق است. به‌منظور اجرای تحقیق 18 سر موش نر پس از 8 هفته تغذیه با رژیم پرچرب، به‌صورت تصادفی در سه گروه کنترل، تمرین تداومی و HIIT تقسیم شدند. تمرین تناوبی شامل تناوب‌های با شدت بالا (vVO2max %80-110) و تناوب‌های با شدت پایین (vVO2max %50) و تمرین تداومی شامل دویدن با شدت (vVO2max %65-75) بود. 48 ساعت پس از آخرین جلسة تمرینی، موش‌ها تشریح و بافت چربی احشایی و زیرپوستی آنها جمع‌آوری و به روش وسترن بلات تجزیه‌وتحلیل شد. نتایج نشان داد که 8 هفته تمرین تداومی و HIIT سبب افزایش معنادار میزان UCP1 در بافت چربی سفید احشایی و زیرپوستی (05/0< P) شد و میزان افزایش در گروه HIIT، صرف‌نظر از نوع بافت احشایی یا زیرپوستی به‌طور معناداری (05/0< P) بیشتر بود. با توجه به نتایج به‌نظر می‌رسد تمرینات HIIT به افزایش بیشتری در میزان UCP1 بافت چربی سفید احشایی و زیرپوستی منجر می‌شوند و افراد چاق می‌توانند با انجام این نوع تمرینات از تأثیرات مفید آن برای جلوگیری از افزایش وزن و بهبود عملکرد بافت چربی بهره‌مند شوند.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Effect of eight weeks of continuous and high intensity interval training on UCP1 in visceral and subcutaneous white adipose tissue of obese rats
نویسندگان [English]
- Maryam Nourshahi 1
- Fatemeh Mir Khalafzadeh 2
- Fariba Khodagholi 3
- Nazanin Abuzari 4
1 Associated professor, Faculty of Physical Education and Sport Sciences, Shahid Beheshti University, Tehran, Iran
2 MSc in exercise physiology , Faculty of Physical Education and Sport Sciences, Shahid Beheshti University, Tehran, Iran
3 Associated professor, Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 PHD student, Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran
چکیده [English]
Prevalence of obesity and its related disease is considered as a major health complication worldwide. Changing the phenotype of white adipose tissue to brown following exercise is currently being studied as one of the strategies to prevent obesity. The aim of this study was to investigate the effect of eight weeks of continuous and high intensity interval training (HIIT) on UCP1 of visceral and subcutaneous white adipose tissue in obese rats. Eighteen obese rats after eight weeks of high fat diet randomly divided into control, continuous training (CT) and HIIT groups. HIIT protocol included high intensity intervals (80-110 % vVO2max) and low intensity intervals (50 % vVO2max) and CT protocol included running with )65-75 % vVO2max( intensity. 48 hours after the last training session, rats were sacrificed, visceral and subcutaneous white adipose tissue were removed and UCP1 level was assessed through western blotting method. The results showed that eight weeks of CT and HIIT increase UCP1 in visceral and subcutaneous WAT (p<0.05), and this increase were higher in HIIT group regardless of visceral or subcutaneous adipose tissue (P<0.005). It seems that HIIT induce higher increase in UCP1 protein in visceral and subcutaneous white adipose tissue, so obese individuals can use this type of training to benefit obesity prevention affects and improvement of adipose tissue function.
کلیدواژه‌ها [English]
- Aerobic Training
- Brown Fat
- Interval Training
- Obesity
- UCP1
Copyright © 2021
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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