تأثیر لیگاسیون عصب نخاعی (SNL) بر بیان ژن‌های TRAF6 و MuRF1 عضلة نعلی موش‌های نر ویستار پس از یک دوره تمرین تناوبی شدید

نوع مقاله : مقاله پژوهشی

نویسندگان

1 . دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکدۀ ادبیات و علوم انسانی، دانشگاه ولی‌عصر (عج) ، رفسنجان، ایران

2 الف) دانشجوی کارشناس ارشد، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، کرمان، ایران، ب) مرکز تحقیقات فیزیولوژی، دانشگاه علوم پزشکی کرمان، کرمان ایران

چکیده

هدف از پژوهش حاضر بررسی اثر لیگاسیون عصب نخاعی (SNL) بر بیان ژن‌های TRAF6 و MuRF1 عضلة نعلی موش‌های نر ویستار پس از یک دوره تمرین تناوبی شدید بود. بدین‌منظور، 24 سر موش نر ویستار در 2 گروه 12 تایی کنترل (C) و تمرین (HIT) قرار گرفتند. گروه تمرین بعد از یک هفته آشناسازی برنامۀ 4 هفته تمرین تناوبی شدید را انجام دادند. سپس به‌طور تصادفی در دو گروه 6 تایی تمرین (HIT) و گروه لیگاسیون عصب نخاعی (HIT-SNL) قرار گرفتند. همزمان گروه کنترل نیز به دو گروه 6 تایی کنترل (C) و لیگاسیون عصب نخاعی (C-SNL) تقسیم شدند. 4 هفته پس از SNL موش‌ها قربانی شدند و عضلة نعلی استخراج و به‌وسیلۀ روش کمی‌سازی داده‌ها Real-time Pcr بیان ژن‌ها اندازه‌گیری شد. نتایج نشان داد که کاهش فعالیت به شکل SNL اثر معنا‌داری بر بیان ژن‌های TRAF6 و MuRF1 دارد (به‌ترتیب 0001/0=P و 0001/0=P). همچنین انجام تمرین ورزشی شدید قبل از SNL به‌طور چشمگیری بیان TRAF6 و MuRF1 را کاهش می‌دهد (به‌ترتیب 003/0=P و 0001/0=P). علاوه‌بر این، یافته‌ها نشان می‌دهد که SNL اثر معنا‌داری بر نسبت وزن عضلة نعلی به طول درشت‌نی داشته است (01/0=P). از طرف دیگر، انجام تمرینات HIT قبل از SNL سبب افزایش معنا‌دار در این نسبت شد (03/0=P).  نتایج نشان داد کاهش فعالیت به شکل SNL با افزایش بیان ژن‌های TRAF6 و MuRF1 همراه است که احتمالاً می‌تواند در تغییر تودة عضلانی نیز درگیر باشد. با توجه به اینکه انجام تمرینات با شدت بالا بیان این ژن‌ها را کاهش می‌دهد، این تمرینات می‌توانند به‌منظور حفظ تودة عضلانی مطلوب‌تر قبل از بی‌فعالیتی بدنی به‌کار روند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Spinal Nerve Ligation (SNL) on the expression of TRAF6 and MuRF1 genes in soleus mucsle of Wistar rats after HIT training

نویسندگان [English]

  • abdolreza kazemi 1
  • Alireza said 2
1 Associate Professor in Exercise Physiology, Department of Sport Sciences, Faculty of Literature and Humanities, Vali E-Asr University, Rafsanjan, Iran
2 . a) MSc Student, Department of Exercise Physiology, Islamic Azad University, Kerman, Iran, b) Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran
چکیده [English]

The purpose of the present study was to investigate the effect of SNL on the expression of TRAF6 muRF1 genes in the Soleus muscle of Wistar rats after HIT traning. For this purpose, 24 male rats were divided into 2 groups of control (C) (n=12) and training (HIT) (n=12). After one-week familiarization, training group participate in four HIT training. Then, they were randomly assigned to HIT (n=6) and HIT-SNL (n=6) groups. At the same time, the control group was divided into two groups: control (C) (n=6) and spinal cord nerve (C-SNL) (n=6). 4 weeks after the SNL, wistar rats were sacrificed and soleuse muscle exetracted.Then Gene expressions of MuRF1 and TRAF6 measured Real time PCR technique. The results showed that inactivity by SNL has a significant effect on the expression of TRAF6 and MuRF1 genes (P=0.0001 and P=0.0001, respectively). Also, performed before SNL reduced the expression of TRAF6 and MuRF1 (P=0.003 and P=0.0001 respectively). In addition, the findings indicated that SNL had a significant effect on the weight of Soleus muscle mass/ tibia length (P=0.01). On the other hand, HIT training before SNL significantly increased weight of Soleus muscle mass/ tibia length (P = 0.03). Therefore, the decrease in activity by SNL is associated with increased in expression of TRAF6 and MuRF1 genes, which may also be involved in muscle mass changes. Regarding the fact that performing HIT reduces the expression of these genes in soleus muscle, these training can be used to maintain obtimal muscle mass before physical activity.

کلیدواژه‌ها [English]

  • SNL
  • تمرین تناوبی
  • MuRF1
  • TRAF6
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