تأثیر همزمان تمرین مقاومتی برون‌گرا و انسداد جریان خون بر بیان ژن STAT3 و MyF5 مؤثر بر فعال‌سازی تکثیر سلول‌های ماهواره‌ای افراد غیرورزشکار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترا فیزیولوژی ورزش، پردیس بین‌الملل کیش دانشگاه تهران، کیش ایران

2 .استاد، گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه تهران، تهران، ایران

3 .استاد، گروه تربیت بدنی دانشگاه تربیت مدرس، تهران، ایران

4 .استادیار، گروه علوم ورزشی، پردیس بین‌المللی کیش دانشگاه تهران، کیش، ایران

5 دانشیار، گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه تهران، ایران

چکیده

هدف از پژوهش حاضر، تأثیر تمرین مقاومتی و انسداد جریان خون بر بیان ژن عامل انتقال دهندة سیگنال و فعال کنندة رونویسی (STAT3) و عامل عضله زایی 5 (Myf5) افراد جوان فعال غیرورزشکار است. 20 مرد با دامنة سنی 25 ± 5 سال به‌صورت تصادفی به دو گروه ده‌نفرة تمرین مقاومتی برون‌گرا با و بدون انسداد جریان خون (BFR) تقسیم شدند.با استفاده از دستگاه ایزوکنتیک، پروتکل تمرین مقاومتی برون‌گرا (ECC RET) شامل حدود 70 تکرار در عضلات بازکننده‌های زانو اجرا شد. انسداد با استفاده از دستگاه فشارسنج بادی ایجاد شد. پس از بی‌حسی موضعی با تزریق لیدوکائین 1% و نوراپی‌نفرین از عضلة پهن جانبی پای فعال، 48 ساعت قبل و 24 ساعت پس از فعالیت نمونه‌برداری شد. STAT3 و MyF5 به روش ایمونوهیستوشیمیایی انجام گرفت. بیان ژن STAT3 در نمونه‌های BFR در گروه پس‌آزمون نسبت به پیش‌آزمون 846/3 برابر بیشتر شده بود که این میزان (001/0=P) تفاوت معناداری را نشان داد. همین‌طور بیان ژن MyF5 در نمونه‌های BFR در گروه پس‌آزمون نسبت به پیش‌آزمون 479/3 برابر بیشتر شده بود که این میزان (005/0=P) تفاوت معناداری را نشان داد. بهره گیری از تمرین مقاومتی برون گرا همراه با انسداد جریان خون از طریق افزایش بیان ژن STAT3 و Myf5 ممکن است سبب حفظ و بهبود تودة عضلانی شود.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Concurrent Effect of Eccentric Resistance Training and Blood Flow Occlusion on STAT3 and MyF5 Gene Expressions Affecting the Activation of Satellite Cells Growth in Non-Athletes

نویسندگان [English]

  • Mohammad Ali Tabibi 1
  • azam mosavian 1
  • abasali gaeini 2
  • R Ghara khanlou 3
  • reza nuri 4
  • mohamadreza kordi 5
1 PhD Student of Exercise Physiology, Kish International Campus, University of Tehran, Kish, Iran
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 Professor, Department of Physical Education, Tarbiat Modares University, Tehran, Iran
4 . Assistant Professor, Department of Sport Sciences, Kish International Campus, University of Tehran, Kish, Iran
5 Associate Professor, Department of Sport Sciences, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
چکیده [English]

The aim of the present study was to investigate the effect of resistance training and blood flow occlusion on gene expression of signal transfer agent and activator of transcription (STAT3) and muscle-forming agent 5 (Myf5) in non-athlete active young subjects. 20 men (age range 25 ± 5 years) were randomly divided into two groups (ach group 10 subjects): eccentric resistance training with and without blood flow restriction (BFR). Using isokinetic set, eccentric resistance training protocol (ECC RET) including about 70 repetitions of knee extensor muscles was performed. Occlusion was created using pneumatic pressure device. After local anesthesia with lidocaine 1% and norepinephrine, blood samples were collected from active vastus lateralis 48 hours before and 24 hours after the training. STAT3 and MyF5 were measured by immunohistochemistry. Gene expression of STAT3 in BFR group in the posttest was 3.846 times higher than the pretest (P=0.001) which showed a significant difference. Gene expression of MyF5 in BFR group in the posttest was 3.479 times higher than the pretest (P=0.005) which showed a significant difference. Eccentric resistance training with blood flow occlusion may maintain and improve muscle mass by increasing the gene expression of STAT3 and MyF5.

کلیدواژه‌ها [English]

  • hypertrophy
  • muscle damage
  • Muscle growth
  • satellite cells
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