اثر تمرین اینتروال شدید بر بیان ژن‌های TRAF6 و MuRF1 در عضلۀ بازکنندۀ طویل انگشتان پای موش‌های پیر

کاظمی, عبدالرضا; بربط, سلامان

doi:10.22059/jsb.2019.262132.1297

نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I

نویسندگان

¹ دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکدۀ ادبیات و علوم انسانی، دانشگاه ولی‌عصر (عج)، رفسنجان، ایران

² کارشناسی ارشد فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکدۀ ادبیات و علوم انسانی، دانشگاه آزاد اسلامی، کرمان، ایران

https://doi.org/10.22059/jsb.2019.262132.1297

چکیده

یکی از سازوکارهای دخیل در آتروفی عضلانی مسیر TRAF6/MuRF1 است که ممکن است تجزیۀ عضلانی را افزایش دهد. ازاین‌رو هدف پژوهش حاضر بررسی اثر تمرین HIIT بر بیان ژن‌های TRAF6 و MuRF1 عضلۀ بازکنندۀ طویل انگشتان پای ((EDL موش‌های پیر C57bl/6 بود. بدین‌منظور، 28 سر موش C57bl/6 پیر (14=n) و بالغ (14=n) هر گروه در 2 گروه تمرین (7=n) و کنترل (7=n) قرار گرفتند که گروه‌های تمرین بعد از یک هفته آشناسازی در برنامۀ 4 هفته HIIT شرکت کردند و 48 ساعت پس از آخرین جلسۀ تمرینی قربانی و عضلۀ EDL استخراج و به‌وسیلۀ روش Real-time Pcr بیان ژن‌ها اندازه‌گیری شد. در مقایسۀ گروه‌های پیر و بالغ، عامل پیری اثر معنا‌داری بر بیان ژن‌های TRAF6 و MuRF1 دارد (به‌ترتیب 005/0=P و 004/0=P)، همچنین تمرین ورزشی به‌طور چشمگیری بیان این دو را تحت تأثیر قرار می‌دهد (0001/0=P). همچنین یافته‌ها نشان می‌دهد که پیری اثر معنا‌داری بر وزن نسبی عضله EDL داشته است، به‌طوری‌که اختلاف معنا‌داری بین گروه‌های بالغ و پیر کنترل (032/0=P) مشاهده شد، ولی این مقدار به لحاظ آماری در هر دو گروه‌های بالغ (117/0=P) و پیر (321/0=P) معنادار نبود. بنابراین، بالا رفتن سن همراه با افزایش بیان ژن‌های TRAF6 و MuRF1 است که احتمالاً در تغییرات تودۀ عضلانی همراه با افزایش سن درگیرند و با توجه به اینکه تمرینات با شدت بالا بیان این ژن‌ها را کاهش می‌دهد، این تمرینات می‌توانند در دوران سالمندی به‌منظور حفظ تودۀ عضلانی استفاده شوند.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of High Intensity Interval Training on Gene Expression of MuRF1 and TRAF6 in Extensor Digitorum Longus (EDL) Muscle of Aged Mice

نویسندگان [English]

Abdolreza Kazemi ¹
Salaman Barbat ²

¹ Associate Professor in Exercise Physiology, Department of Sport Sciences, Faculty of Literature and Humanities, Va-i E-Asr University, Rafsanjan, Iran

² MSc in Exercise Physiology, Department of Exercise Physiology, Faculty of Literature and Humanities, Islamic Azad University, Kerman , Iran

چکیده [English]

One of the mechanisms involved in muscle atrophy is TRAF6/MuRF1 path, which may increase muscle breakdown. The aim of the present study was to investigate the effect of high intensity interval training (HIIT) on gene expression of MuRF1 andTRAF6in EDL muscle of aged C57bl/6 mice. For this purpose, 28 C57bl/6 aged (n=14) and adult (n=14) mice of each group were assigned to two groups: training (n=7) and control (n=7). After one week of familiarization, training groups participated in 4 weeks of HIIT program. The mice were sacrificed 48 hours after the last training session and their EDL muscle were extracted and the gene expressions were measured with Real-time PCR technique. The comparison of aged and adult groups showed that aging had a significant effect on MuRF1 and TRAF6 mRNA gene expression (P=0.005 and P=0.004 respectively). Also, training drastically influenced MuRF1 and TRAF6 expression (P=0.0001). The findings also showed that aging had a significant effect on EDL muscle weight as a significant difference was observed between aging and adult control groups (P=0.032). But this amount was not statistically significant in both adult (P=0.117) and aged (P=0.321) groups. Thus, aging is associated with an increase in MuRF1 andTRAF6gene expression, which could possibly be involved in muscle mass changes associated with aging. Since high intensity interval training decrease the expression of these genes, it can be utilized to maintain muscle mass during aging.

کلیدواژه‌ها [English]

Aging
high intensity interval training
MuRF1
muscle mass
TRAF6

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