اثر 4 هفته تمرین هوازی بر سطوح سرمی و کبدی نئورگولین-4 موش‌های صحرایی دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی ، دانشگاه مازندران، بابلسر، ایران

2 . دانشیار گروه فیزیولوژی ورزشی، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

چکیده

دیابت با افزایش لیپوژنز و بیان ژن‌های آن در کبد و فعالیت نئورگولین-4 با تضعیف لیپوژنز کبدی همراه است. هدف پژوهش حاضر بررسی تأثیر 4 هفته تمرین هوازی بر تغییرات سطوح سرمی و کبدی نئورگولین-4 و نیمرخ لیپیدی موش‌های دیابتی بود. 32 سر موش صحرایی نر بالغ، به‌صورت تصادفی به گروه‌های هشت‌تایی کنترل سالم، کنترل دیابتی، تمرین و دیابتی تمرین تقسیم شدند. القای دیابت با تزریق درون‌صفاقی محلول استرپتوزوتوسین (mg/kg 55) انجام گرفت. فرایند تمرین به مدت 4 هفته (5 جلسه در هفته) با سرعت 15 تا 18 متر در دقیقه و برای 25 تا 44 دقیقه اجرا شد. 48 ساعت پس از آخرین جلسۀ تمرینی، خون‌گیری و بافت‌برداری انجام گرفت و سطوح نئورگولین-4 اندازه‌گیری شد. تفاوتی در سطوح سرمی و کبدی نئورگولین-4 بین گروه‌های دیابتی و غیردیابتی مشاهده نشد (05/0<P). سطوح سرمی کلسترول و HDL بین گروه‌های مذکور تفاوت معناداری نداشت (05/0<P). تری‌گلیسرید و LDL بین این گروه‌ها با افزایش معنادار همراه بود (05/0>P). 4 هفته تمرین هوازی بر سطوح سرمی و کبدی نئورگولین-4 موش‌های دیابتی و سالم تأثیری نداشت (05/0<P). همچنین 4 هفته تمرین هوازی بر سطوح سرمی کلسترول، تری‌گلیسرید، HDL و LDL در موش‌های سالم مؤثر نبود (05/0<P). تمرین در موش‌های دیابتی موجب کاهش معنادار سطوح سرمی کلسترول و تری‌گلیسرید شد (05/0>P)، ولی تفاوت معناداری در HDL و LDL ایجاد نکرد (05/0<P). با اینکه سطوح نیمرخ لیپیدی دچار تغییرات محسوس شد، احتمالاً تمرینات ورزشی هوازی در 4 هفته نمی‌تواند محرک افزایش نئورگولین-4 گردشی و بافتی کبد باشد.
 

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 4 Weeks of Aerobic Exercise on Serum and Liver Levels of Neuregulin-4 in Diabetic Rats

نویسندگان [English]

  • Seyyed Pedram Rouhani Doost 1
  • Rozita Fathi 2
1 MSc Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Mazandaran University, Babolsar, Iran
2 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Mazandaran University, Babolsar, Iran
چکیده [English]

­Diabetes is associated with increased lipogenesis and expression of lipogenic genes in the liver, and neuregulin-4 activity is associated with inhibition of liver lipogenesis. The aim of this study was to investigate the effect of 4 weeks of aerobic exercise on changes of serum and liver levels of neuregulin-4and lipid profile in diabetic rats. ­32 male adult rats were randomly divided into healthy control, control diabetic, exercise and exercise diabetic groups (each group 8 rats). Diabetes was induced by intraperitoneal injection of streptozotocin solution (55 mg/kg). The exercise was performed for 4 weeks, 5 sessions per week, at speeds of 15 to 18 m/min. and for 25 to 44 minutes. 48 hours after the last exercise session, blood samples were collected and liver tissue was removed and Neuregulin-4 levels were measured. The results showed no difference in serum and liver levels of neuregulin-4 between diabetic and healthy groups (P>0.05). Serum levels of cholesterol and HDL were not significantly different among the above groups (P>0.05). Triglyceride and LDL were significantly higher among these groups (P<0.05). 4 weeks of aerobic exercise did not affect serum and liver levels of neuregulin-4 in diabetic and healthy rats (P>0.05). Also, 4 weeks of aerobic exercise did not affect serum levels of cholesterol, triglyceride, HDL and LDL in healthy rats (P>0.05). Exercise in diabetic rats significantly decreased serum levels of cholesterol and triglyceride (P<0.05) but it did not make any significant differences in HDL and LDL (P>0.05). ­Although the levels of lipid profile changed significantly,  4 weeks of aerobic exercise probably cannot stimulate increases of neuregulin-4 in serum and liver tissue.

کلیدواژه‌ها [English]

  • aerobic exercise
  • diabetes
  • lipid profile
  • liver lipogenesis
  • neuregulin-4
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