تأثیر شش هفته تمرین هوازی بر شاخص های نروژنز و نروتروفیک در هیپوکمپ رت های جوان و میانسال

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده ی علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 دانشیار،گروه فیزیولوژی ورزشی، دانشکده ی علوم ورزشی، دانشگاه مازندران، بابلسر، ایران.

چکیده

هدف از این پژوهش یافتن پاسخ این پرسش‌ها بود: آیا ورزش می‌تواند نروژنز را افزایش دهد و فاکتور نروتروفیک که برای نروژنز ضروری است آیا با ورزش افزایش می‌یابد؟30 سر موش صحرایی به چهار گروه کنترل جوان، تمرین جوان، کنترل میانسال و تمرین میانسال تقسیم شدند. تمرین براساس اصل اضافه‌بار به مدت 6 هفته و 6 جلسه در هفته انجام گرفت. رت‌های جوان با سرعت 27 متر بر دقیقه و رت‌های میانسال با سرعت 20 متر بر دقیقه به مدت 20 دقیقه در روز اول دویدند. هر روز 2 دقیقه به زمان دویدن اضافه شد تا به 60 دقیقه در روز رسید. شاخص‌ها با روش الایزا اندازه‌گیری و از طریق روش آماری آزمون تعقیبی tukey تجزیه‌وتحلیل شد.بین وزن گروه‌های جوان تفاوت معنا‌داری وجود نداشت (sig=0.979) و تفاوت بین گروه‌های میانسال معنا‌دار بود (sig=0.000). Ki67 در گروه تمرینی جوان و میانسال به‌طور معنا‌داری از گروه کنترل بیشتر بود؛ به‌ترتیب (sig=0.002) و (sig=0.037). Midkine در گروه تمرینی جوان در مقایسه با گروه کنترل جوان افزایش معنا‌دار نداشت (sig=0.134). این شاخص در گروه تمرینی میانسال نیز افزایش یافت، ولی افزایش معنا‌داری نداشت (sig=0.557). همبستگی بین Midkine و Ki67 معنا‌دار بود (r=0.407) (sig=0.029). تمرین مداوم می‌تواند نروژنز را در رت‌های جوان و میانسال افزایش دهد. احتمالاً این نوع تمرین برای افزایش نروژنز و ملزومات آن یعنی فاکتورهای نروتروفیک مفید است.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 6 Weeks of Aerobic Training on Neurogenesis and Neurotrophic Factors in the Hippocampus of Young and Middle-Aged Rats

نویسندگان [English]

  • Razieh Mohammadi 1
  • ziya fallahmohammadi 2
1 PhD student, exercise physiology group, the faculty of sport science, university of Mazandaran,Banolsar,Iran.
2 associated professor, Group of exercise physiology, faculty of exercise science, university of Mazandaran, Babolsar, Iran.
چکیده [English]

The aim of this study was to find answers to these questions: can exercise increase neurogenesis and does neurotrophic factor that is necessary for neurogenesis increase with training?30 rats were divided into four groups: young control, young training, middle-aged control and middle-aged training. The training was performed with overload principle for 6 weeks and 6 sessions per week. Young rats ran with the speed of 27 m/min. and the middle-aged rats with the speed of 20 m/min. for 20 minutes on the first day. The running time increased 2 minutes every day until it reached 60 minutes per day. Elisa method was used to measure the factors and they were analyzed by Tukey post hoc test.There was no significant difference in weight between young groups (sig=0.979) but the difference was significant between middle-aged groups (sig=0.000). Ki67 in young and middle-aged training groups was significantly more than control group (sig=0.002) and (sig=0.037). Midkine did not have a significant increase in the young training group compared with the young control group (sig=0.134). This factor increased in middle-aged training group but this increase was not significant (sig=0.557). The correlation between ki67 and Midkine was significant (r=0.407) (sig=0.029).Continuous training can increase the neurogenesis in young and middle-aged rats. This type of training may be useful to increase neurogenesis and its essentials (i.e. neurotrophic factors).
 

کلیدواژه‌ها [English]

  • Hippocampus
  • ki67
  • Midkine
  • running on treadmill
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