تأثیر چهار هفته تمرین ورزشی داوطلبانه بر رفتار شبه‌افسردگی ناشی از استرس دوران کودکی و استرس اکسیداتیو موش‌های صحرایی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بیوشیمی و متابولیسم ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، واحد تهران مرکزی، تهران، ایران

2 استاد گروه فیزیولوژی ورزش، دانشکده تربیت بدنی وعلوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکزی،تهران، ایران

چکیده

هدف پژوهش حاضر بررسی تأثیر چهار هفته دویدن اختیاری روی چرخ دوی گردان در دوران نوجوانی بر رفتار افسردگی و تأثیر آن بر میزان استرس اکسیداتیو در مخچه است. به همین منظور 24 سر موش نر به‌صورت تصادفی به سه گروه کنترل، جداشده از مادر (MS) و جداشده از مادر و دویدن اختیاری (MS+RW) تقسیم شدند. موش‌های گروه  MS و MS+RW از روز دوم پس از تولد تا روز چهاردهم به مدت 180 دقیقه از مادرشان جدا شدند. سپس آزمودنی‌ها به‌صورت چهارتایی تا روز بیست‌وهشتم در قفس نگهداری شدند. گروه MS+RW از روز بیست‌وهشتم به چرخ گردان به‌صورت 24 ساعت شبانه‌روز دسترسی داشت. در روز شصتم بعد از تولد رفتارهای افسردگی به‌وسیلۀ آزمون‌های FST، SPT،
OPT، و Splash test ارزیابی شد. بیومارکر استرس اکسیداتیو که شامل میزان ROS میتوکندریایی در بافت مخچه بود نیز اندازه‌گیری شد. نتایج نشان داد دویدن اختیاری به‌طور معنا‌داری رفتارهای شبه‌افسردگی ناشی از استرس جداسازی از مادر را خنثی کرد و استرس اکسیداتیو را کاهش داد. همچنین نتایج نشان داد دویدن اختیاری می‌تواند به‌عنوان روش درمانی غیردارویی برای اختلالات افسردگی مفید باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 4 Weeks of Voluntary Training on Depression-Like Behavior Induced by Childhood Stress and Oxidative Stress in Rats

نویسندگان [English]

  • Abolfazl Jafarzadeh Baghan 1
  • Maghsoud peeri 2
  • mohammad Ali Azarbayjani 2
1 PhD Student of Biochemistry and Exercise Metabolism, Faculty of Physical Education and Sport Sciences, Islamic Azad University, Central Tehran Branch, Tehran, Iran
2 Professor, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University,Tehran, Iran
چکیده [English]

The aim of this study was to determine the effect of four weeks of voluntary running on running wheel (RW) in adolescence on depression and its effect on oxidative stress in the cerebellum. For this purpose, 24 male rats were randomly divided into 3 groups: control, separated from mother (MS) and separated from mother and running wheel (MS + RW). MS and MS + RW rats were separated from their mothers from postnatal day (PND) 2 to 14 for 180 min. per day. Then, each 4 rats per cage were kept until the 28th day. MS + RW group had access to the running wheel 24 hours a day from the 28th day. At PND 60, depressive-like behaviors were assessed by Open Field Test (OPT), Sucrose Preference Test (SPT), Forced Swimming Test (FST) and Splash test. Biomarker of oxidative stress including mitochondrial reactive oxygen species (ROS) in the cerebellum tissue was also measured. The results showed that voluntary running significantly neutralized depression-like behaviors induced by the maternal separation stress and reduced oxidative stress. Also, the results showed that voluntary running could be useful as a non-pharmacological treatment for depression.

کلیدواژه‌ها [English]

  • Depression-like behavior
  • early life stress
  • oxidative stress
  • voluntary running

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