تأثیر تمرینات تناوبی فزاینده بر آپوپتوز قلب رت‌های جوان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد، دکتری تخصصی فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی دانشکدة علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 کارشناسی ارشد فیزیولوژی ورزشی، ساری، ایران

3 استادیار، دکتری تخصصی بافت‌شناسی، گروه علوم پایۀ دانشکدة دامپزشکی، دانشگاه تبریز، تبریز، ایران

چکیده

تمرینات تناوبی ممکن است آثار حفاظتی قلب در برابر مرگ سلولی را با چالش مواجه کند، با این حال نقش حفاظتی تمرینات ورزشی در پیشگیری از آپوپتوز ناشناخته است. هدف پژوهش حاضر بررسی تأثیر تمرین تناوبی فزاینده بر آپوپتوز بافت قلب رت‌های جوان بود.30 سر موش صحرایی نر ویستار جوان به‌طور تصادفی به سه گروه تقسیم شدند. برنامۀ تمرین شامل 6 هفته تمرین تناوبی به مدت 6 جلسه در هفته، هر جلسه 30 دقیقه با سرعت 25 تا 70 متر بر دقیقه با 10 تکرار 1 دقیقه‌ای و استراحت فعال دودقیقه‌ای بود. 48 ساعت پس از آخرین جلسۀ تمرینی نمونه‌برداری از قلب انجام گرفت. برای مطالعۀ آپوپتوزی ساختار بافت قلب، در محلول فیکساتیو فرمالین 10 درصد قرار داده شد. برای تشخیص سلول‌های آپوپتوزی، از روش غیر رادیواکتیو نشاندار و تجزیه‌وتحلیل آماری با استفاده از تحلیل واریانس یکطرفه و آزمون تعقیبی بن‌فرونی در سطح معنا‌داری (005/0P≤) انجام گرفت.نتایج ایمونوهیستوشیمی نشان داد 6 هفته تمرین تناوبی فزاینده موجب افزایش 200و 320 درصدی معنا‌دار (001/0P≤) آپوپتوز گروه تمرین در مقایسه با گروه کنترل شش هفته و گروه پایه شد. با توجه به ترسیم بدیع برهم‌کنش وابسته به سن در این مطالعه که به فعال‌سازی مسیر آپوپتوزیس در پی تمرینات تناوبی فزاینده منجر شد، بروز چنین واکنشی در کودکان و نوجوانان روشن نیست و نیازمند مطالعات آینده است، بنابراین توجه به ملاحظات تمرینی در طراحی تمرینات تناوبی برای آنان ضروری است.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of High Intensity Interval Training on Heart Apoptosis of Young RatsThe Effect of High Intensity Interval Training on Heart Apoptosis of Young Rats

نویسندگان [English]

  • Shadmehr Mirdar 1
  • Niloofar Moghadasi 2
  • Gholamreza Hamidain 3
1 Professor, PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran
2 . Msc of Exercise Physiology, Sari, Iran
3 Assistant Professor, PhD of Comparative Histology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
چکیده [English]

Interval training may challenge the protective effect of heart against cell death. However, the protective role of exercise in preventing apoptosis is unknown. The aim of this study was to investigate the effect of high intensity interval training on apoptosis in young rats’ heart. 30 male young Wistar rats were randomly divided into three groups. The training program included six weeks of interval training, six sessions per week, 30 minutes each session with the speed of 25-70 m/min. with 10 one-minute repetitions and 2-minute active rest. Sampling of the heart tissue was conducted 48 hours after the last training session. In order to investigate the apoptosis, the heart tissue was placed in 10% formalin fixative solution. The apoptotic cells were labeled with non-radioactive method. The one-way ANOVA and Bonferroni post hoc test at the significance level (P≤0.005) were used for data analysis. Immunohistochemistry results showed that 6 weeks of HIIT significantly increased 320 and 200% (P≤0.001) of apoptosis in experimental group compared with the 6-week control group and baseline group. These findings delineate a novel, age‐dependent interaction which activated apoptotic pathway by HITT, but such reaction is unclear in adolescents and children and further research is needed. Thus it is necessary to consider training concerns when designing HITT for them.

کلیدواژه‌ها [English]

  • Cell death
  • exercise
  • Heart
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