نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I

نویسندگان

1 .دانشجوی دکتری فیزیولوژی ورزشی، پردیس دانشگاهی، دانشگاه گیلان، رشت، ایران

2 .استاد، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران

3 دانشجوی دکتری فیزیولوژی ورزشی گروه فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران

4 دانشجوی دکتری فیزیولوژی ورزشی، پردیس دانشگاهی، دانشگاه گیلان، رشت، ایران

چکیده

هدف از پژوهش حاضر مقایسۀ  تأثیر 12 هفته تمرین تناوبی با شدت بالا و متوسط بر پری‌لیپین3 (PLIN3) بافت چربی احشایی و مقاومت به انسولین رت‌های نر دیابتی نوع 2 بود. در این مطالعه 24 سر رت نر دیابتی نوع 2 به‌صورت تصـادفی بـه سـه گـروه تمرین تناوبی با شدت متوسط (MIIT)، تمرین تناوبی شدت بالا (HIIT) و کنتـرل تقسـیم شـدند. تمرینات تناوبی (HIIT ، MIIT) به مدت 12 هفته و 5 جلسه در هفته اجرا شد. پروتکل MIIT شامل اجرای 13 وهله فعالیت چهاردقیقه‌ای با شدت 70-65 درصد VO2max و پروتکل HIIT شامل اجرای 10 وهله فعالیت چهاردقیقه‌ای با شدت 90-85 درصد VO2max با دوره‌های استراحتی  فعال دودقیقه‌ای بود. از روش وسترن بلات برای اندازه‌گیری سطوح پروتئینی PLIN3 استفاده شد. از آزمون‌های ANOVA و آزمون توکی برای تحلیل داده‌ها استفاده شد. نتایج تحلیل داده‌ها بیانگر کاهش معنا‌دار PLIN3 در گروه‌های HIIT وMIIT  نسبت بـه گـروه کنتـرل بـود (001/0=P)، با این‌ حال، تفاوت معنا‌داری بین گروه‌های تمرینی وجود نداشت (274/0=P). همچنین، هر دو پروتکل HIIT و MIIT به  کاهش معناداری در سطوح سرمی گلوکز منجر شدند (001/0=P)، درحالی‌که انسولین سرمی کاهش و تأثیر معنا‌داری بر مقاومت به انسولین نداشتند (05/0˃P).  همچنین، تفاوت معنا‌داری بین گروه‌های تمرینی در تغییرات انسولین، گلوکز و مقاومت به انسولین وجود نداشت (05/0˃P). یافته‌های پژوهش حاضر نشان داد که هر دو پروتکل HIIT و MIIT موجب کاهش چشمگیر PLIN3 چربی احشایی و بهبود متابولیسم گلوکز در رت‌های دیابتی نوع 2 می‌شوند. بنابراین، به‌نظر می‌رسد که تغییرات PLIN3 چربی احشایی مستقل از شدت فعالیت ورزشی است.

کلیدواژه‌ها

عنوان مقاله [English]

A Comparison of the Effect of 12 Weeks of Interval Training with High and Moderate Intensity on Perilipin 3 of Visceral Adipose Tissue and Insulin Resistance in Type 2 Diabetic Male Rats

نویسندگان [English]

  • Kamilia Moghaddami 1
  • Hamid Mohebbi 2
  • Elma Tabari 3
  • Mehrsa Faridnia 4

1 PhD Student of Exercise Physiology, University Campus, Guilan University, Rasht, Iran

2 Professor, Faculty of Physical Education and Sport Sciences, Guilan University, Rasht, Iran

3 PhD Student of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Guilan University, Rasht, Iran

4 Professor, Faculty of Physical Education and Sport Sciences, Guilan University, Rasht, Iran

چکیده [English]

The aim of this study was to compare the effect of 12 weeks of interval training with high and moderate intensity on perilipin 3 (PLIN3) of visceral adipose tissue and insulin resistance in type 2 diabetic male rats. In this study, 24 male rats with type 2 diabetes were randomly divided into three groups: interval training with moderate intensity (MIIT), interval training with high intensity (HIIT) and control. Interval training (HIIT, MIIT) was applied for 12 weeks, 5 sessions per week. The MIIT protocol included 13 four-min. bouts of activity with intensity of 65-70% vo2max and the HIIT protocol included 10 four-min. bouts of activity with intensity of 85-90% vo2max with 2-minute active rest periods. Western Blot method was used to measure PLIN3 protein levels. ANOVA and Tukey's test were used for data analysis. The results indicated a significant decrease in PLIN3 in the HIIT and MIIT groups compared with the control group (P=0.001). However, there was no significant difference between the training groups (P=0.274). Also, both HIIT and MIIT protocols significantly decreased serum levels of glucose (P=0.001), while they had no significant effects on serum insulin and insulin resistance (P˃0.05). There was no significant difference between training groups in insulin, glucose and insulin resistance changes (P˃0.05). The findings of this study showed that both HIIT and MIIT protocols drastically reduced PLIN3 of visceral adipose and improved glucose metabolism in type 2 diabetic rats. Therefore, it seems that PLIN3 changes in visceral adipose are independent of the training intensity.

کلیدواژه‌ها [English]

  • insulin resistance
  • interval training
  • PLIN3
  • type 2 diabetes
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