تأثیر فعالیت ورزشی هوازی بر شاخص‌های آپوپتوزی و رشدی در قلب رت‌های پیر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار گروه فیزیولوژی ورزش دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه تهران، تهران، ایران

2 دانشجوی دکتری فیزیولوژی ورزش، دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه تهران، تهران، ایران

چکیده

پیری با افزایش آپوپتوز مشخص می‌شود. به‌نظر می‌رسد که فعالیت ورزشی هوازی می‌تواند آپوپتوز را کاهش دهد. هدف از این مطالعه بررسی تأثیر تمرین ورزشی هوازی بر شاخص‌های آپوپتوزی و رشدی در قلب رت‌های پیر بود. 24 سر رت ویستار نر به دو گروه تمرین هوازی شدت بالا (8 سرHIT:) و شدت متوسط ( 8 سرMCT:) و یک گروه کنترل (8 سرCO:) تقسیم شدند. رت‌ها پس از دو هفته دورۀ سازگاری به مدت شش هفته تمرین هوازی انجام دادند. قلب رت‌ها پس از 48 ساعت از آخرین جلسۀ تمرینی استخراج شد. بیان ژن Bax و Bcl-2 به روش Real-time-PCR آنالیز شد. برای آنالیز داده‌ها از روش آماری تحلیل واریانس یکطرفه با معناداری 05/0P< استفاده شد. نتایج نشان داد که مقادیر بیان ژن Bax در گروه تمرین کم‌شدت نسبت بـه گروه کنترل افزایش غیرمعنادار (261/0)، اما در گـروه تمرین شدید افزایش معناداری داشت (001/0). مقادیر ژن Bcl-2 در گروه تمرین کم‌شدت (978/0) و شدید (278/0) نسبت بـه گروه کنترل افزایش غیرمعناداری را نشان داد. مقدار IGF-1 در گروه تمرین کم‌شدت (175/0) و شدید (537/0) نسبت بـه گروه کنترل افـزایش نشان داد، اما معنادار نبود. با توجه به نتایج، شش هفته فعالیت ورزشی با شدت کم و شدید موجب افزایش غیرمعنادار در بیان Bcl-2، IGF-1 در دو شدت تمرینی در رت‌های سالمند شده، همچنین به افزایش معنادار Bax در گروه تمرین شدید و افزایش غیرمعنادار در گروه کم‌شدت می‌انجامد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Aerobic Training on Apoptosis and Growth Factors in Old Rats’ Hearts

نویسندگان [English]

  • Rahman Souri 1
  • amir alahyar 2
  • fatemeh shabkhiz 1
  • arezoo eskandari 2
1 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
2 PhD Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
چکیده [English]

Aging is characterized by an increase in apoptosis. It seems that aerobic training can reduce the apoptosis. The aim of this study was to examine the effect of aerobic training on apoptosis and growth factors in the old rats’ hearts. 24 male Wistar rats were divided into 3 groups: high intensity training (HIT=8), moderate intensity training (MCT=8) and control (CO=8). After two weeks of adaptation, rats participated in aerobic training for 6 weeks. The rats’ hearts were extracted 48 hours after the last training session. Bax and Bcl-2 gene expressions were analyzed by the Real-Time-Polymerase Chain Reaction (RT-PCR). One-way analysis of variance was used for data analysis (P<0.05). The results showed that Bax gene expression in MCT group insignificantly increased (0.261) but in HIT group significantly increased (0.001) compared with the control group. Bcl-2 gene levels in MCT group (0.978) and HIT group (0.278) insignificantly increased compared with the control group. IGF-1 in MCT group (0.175) and HIT group (0.537) increased compared with the control group, but this increase was not significant. According to the results, 6 weeks of training with moderate and high intensity insignificantly increased the expression of Bcl-2 and IGF-1 in elderly rats and significantly increased Bax in high intensity training group and insignificantly increased it in the moderate intensity group.

کلیدواژه‌ها [English]

  • Aging
  • Apoptosis
  • Bax
  • Bcl-2
  • Heart
  • insulin-like growth factor-1
  • Training
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