نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دکتری فیزیولوژی ورزشی دانشگاه تهران، تهران، ایران
2 .استادیار گروه فیزیولوژی ورزشی دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه تهران، تهران، ایران
3 .استادیار گروه فیزیولوژی ورزشی دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه الزهرا(س)، تهران، ایران
چکیده
نوع فعالیت بدنی انجام‌گرفته تأثیر بسزایی در میزان آسیب اکسایشی و پاسخ دفاعی بدن دارد. فعالیت بدنی منظم می‌تواند به‌عنوان یک واکسن عمل کند و سبب تنظیم مثبت سیستم‌های ضد اکسایشی بدن شود. در این تحقیق 40 موش صحرایی نر نژاد ویستار به‌طور تصادفی به دو گروه تمرینی و کنترل تقسیم شدند که برای گروه تمرینی هشت هفته تمرین استقامتی با شدت 75-60% حداکثر اکسیژن مصرفی و 5 روز در هفته در نظر گرفته شد. پس از پایان هشت هفته گروه تمرینی و گروه کنترل هر یک به دو گروه تقسیم شدند که یک گروه از هر کدام، یک جلسه تمرین تا حد واماندگی را اجرا کردند. فعالیت آنزیم آنتی‌اکسیدانی کاتالاز (CAT) گلبول‌های قرمز و میزان مالون دی آلدهید (MDA)و ظرفیت آنتی‌اکسیدانی تام سرم اندازه‌گیری شد. اطلاعات به‌دست‌آمده از طریق تجزیه‌وتحلیل واریانس ANOVA و آزمون تعقیبی LSD با سطح معناداری 05/0 تجزیه‌وتحلیل شدند. یافته‌های پژوهش نشان داد که میزان MDA تنها در گروه تمرین وامانده‌ساز افزایش معنادار داشته است (007/0P=). فعالیت کاتالاز در گروه تمرین استقامتی افزایش معناداری را نشان داد(001/0 =P). تفاوت معناداری در ظرفیت آنتی‌اکسیدانی تام گروه تمرین استقامتی + یک جلسه تمرین وامانده‌ساز و گروه کنترل + یک جلسه تمرین وامانده‌ساز وجود داشت (04/0=P). می‌توان نتیجه‌گیری کرد که تمرین وامانده‌ساز میزان آسیب اکسایشی را افزایش داده، اما تمرین استقامتی توانسته است آثار مضر ناشی از تمرین وامانده‌ساز و استرس ناگهانی را کاهش دهد.
کلیدواژه‌ها
عنوان مقاله [English]
The Effect of Endurance Training on Prevention of Harmful Effect of Oxidative Stress Induced by Exhaustive Exercise in Rats
نویسندگان [English]
- Sahar Daneshvarzavajery 1
- Parisa Pournemati 2
- Niku Khosravi 3
1 .Ph.D of physical education and sport science, University of Tehran, Tehran, Iran
2 Assistant Professor of Sport Physiology department, Faculty of physical education and sport sciences, University of Tehran, Tehran, Iran
3 . Sport Physiology department, Faculty of physical education and sport sciences, Alzahra University, Tehran, Iran
چکیده [English]
Different protocols of exercise cause different oxidative status. Regular exercises could act like vaccination and up regulate antioxidant systems in body. So 40 male Wistar rats with body weight (154±2 gr) were divided into two groups: 1) trained (n=20) and 2) untrained groups (n=20) at random. Training group participated in eight weeks training program, 5days per week, consisted of treadmill running that began speed was 8 m/min for 10 min and with gradually increase in speed and time reaching the speed of 30 m/min for 1.5 h/day at 8th week.At the end of 8 week each group further divided in to groups where the rats were studied at rest and after exhaustive exercise. For acute exhaustive exercise graded treadmill running was conducted reaching the speed of 35 m/min and was continued until exhaustion, then animals were anesthetized and blood sample were collected for measurements of catalase activity in red blood cell by Abei method and plasma MDA was estimated by spectrophotometry and total antioxidant capacity (TAC) measured by Ferric reducing antioxidant power (FRAP). These results indicated that acute exhaustive exercise increased the MDA level in sedentary but not in trained rat. The catalase activity was elevated significantly in trained group but was unchanged by exhausting exercise and TAC increased in exercise group after exhaustive exercise but decreased in just one session exhaustive group. We conclude that exhaustive exercise induced oxidative stress but endurance training may be useful for preventing acute exhaustive exercise induced oxidative stress by up-regulating antioxidant enzyme activity.
کلیدواژه‌ها [English]
- Total anti-oxidant capacity
- Catalase
- Endurance training
- exhaustive exercise
- oxidative stress
- Honarmand M, Nakhaei A, Shad M. Comparison of Total Antioxidant Capacity of Serum in Type 2 Diabetic Patients and Healthy Individuals. JSSU. 2014;21(6):742-50.
- Miyazaki H, Ohishi S, Ookawara T, Kizaki T, Toshinai K, Ha S, et al. Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. Eur J Appl Physiol. 2001;84(1-2):1-6.
- de Castro M, Calvacanti, Neto F, Lima L, da Silva F, de Oliviera R, et al. Production of free radicals and catalase activity during acute exercise training in young men. Biol Sport. 2009;26:113-8.
- Revan s, Erol A. Effects of endurance training on exhaustive exercise induced oxidative stress markers. African journal of pharmacy and pharmacology. 2011;5(3):437-41.
- Modir M, Daryanoosh F, Tanideh N, Mohamadi M, Firouzmand H. The effects of short and middle times aerobic exercise with high intensities on ingredients antioxidant in female Sprague Dawley rats. Medical Journal of Mashhad University of Medical 2014;57(3):587-95.
- Afzalpour M, M S, A Z, M J. Comparison of the effects of an acute resistance and aerobic exercise session on the antioxidant defense system and lipid peroxidation of healthy young men. Journal of Sport in Biomotor Sciences. 2012;6(2):39-50.
- Margaritis I, Palazzetti S, Rousseau A, Richard M, A F. A antioxidant supplementation and tapering exercise improve exercise- induced antioxidant response. J Am Coll Nutr. 2003;22(2):147-56.
- Show Y. Free radical scavenging capacity and antioxidant enzyme activity in deerberry (vacciniumstamineuml). J Agricultrual Food Chemistry. 2006;58:233-41.
- Castell L, Gough L, Johnston M, Armstrong K, J K, R G, editors. Antioxidant capacity of serum, saliva and urine before and after acute exercise: A pro-oxidant response. 12th Annual congress of the ECSS; 2007; Finland.
- Leelarugrayubl N, Sutabhaha T, Pothongsunun P, Chanarat N. Exhaustive Exercise test and oxidative stress response in athletic and sedentary subjects. CMU Journal. 2005;4(2):182-5.
- Somani S, Frank S, P R. Response of antioxidant system to acute and Trained exercise in rat heart subcellular fractions. Pharmacology Biochemistry and Behavior. 1995;51(4):627-34.
- Oztasan N. Endurance training attenuates exercise – induced oxidative stress in erythrocytes in rat. Eur JApplPhysiol. 2004;91:622-7.
- Sentürk U, Gündüz F, Kuru O, Aktekin M, Kipmen D, Yalçin O, et al. Exercise-induced oxidative stress affects erythrocytes in sedentary rats but not exercise-trained rats. J Appl Physiol (1985). 2001;91(5):1999-2004
- Taysi s, Oztasan n, Efe H, Polat M, Gumustekin K, Siktar E, et al. Endurance training attenuates the oxidative stress due to acute exhaustive exercise in rat liver. Acta Physiol Hung. 2008;95(4):337-47.
- Ferrari G, Ferrari c. Exercise modulation of total antioxidant capacity (TAC): towards a molecular signature of healthy aging. Frontiers in Life Science. 2012;5(3):81-90.
- Gul M, Laaksonen D, Atalay M, Vider L, Hänninen O. Effects of endurance training on tissue glutathione homeostasis and lipid peroxidation in streptozotocin-induced diabetic rats. Scand J Med Sci Sports. 2002;12(3):163-70.
- Shemshaki A, Ghanbari Niaki A, Rajabi H, Hedayati M, Salami F. Intense Alpine Skiing Exercise on Anti Oxidant Status of Male Skiers. Shaheed Beheshti University of Medical Sciences & Health Services Endocrine & Metabolism Research Center. 1386;9(3):291-7.
)