تأثیررژیم غذایی پرچرب غیر اشباع و تمرین هوازی بر سطوح سرمی ایزوفرم های منتخب آدیپونکتین، مقاومت به انسولین و نیم‌رخ لیپیدی در موش‌های نر چاق

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار دانشگاه گیلان، رشت، ایران

2 استاد دانشگاه گیلان، رشت، گیلان

3 استاد دانشگاه گیلان رشت ، گیلان

4 گروه بافت شناسی، دانشگاه علوم پزشکی، دانشگاه گیلان، رشت، ایران

چکیده

هدف از مطالعه حاضر، بررسی تأثیر 10 هفته تمرین هوازی و رژیم غذایی پرچرب غیراشباع بر سطوح سرمی آدیپونکتین در موش‌های نر چاق می‌باشد. نمونه‌برداری خونی از 48 سر موش صحرایی نر نژاد ویستار(با میانگین وزن 5±195 گرم و سن 8 هفته) پس از ناشتایی شبانه در هفته‌های اول، 18 و 28 صورت گرفت. پس از نمونه-برداری پایه،40 سر موش باقیمانده بطور تصادفی به گروه کنترل(16 سر) و رژیم غذایی پرچرب(24 سر) تقسیم شدند. پس از 18 هفته گروه پرچرب به زیرگروه‌های ورزشی و رژیم پرچرب تقسیم شد. گروه ورزشی به مدت 10 هفته(5 جلسه در هفته)، تمرین هوازی با شدت معادلVo2max 75-70% انجام داد. غلظت‌های سرمی آدیپونکتین تام و HMW و همچنین متغیرهای نیم‌رخ لیپیدی و شاخص‌های گلیسمیک اندازه‌گیری شدند. نتایج این مطالعه نشان داد که تمرین هوازی تحت رژیم پرچرب در مقایسه با غذای پرچرب موجب افزایش معنی‌دار سطوح سرمی آدیپونکتین تام گردید(01/0>P). همچنین تمام شاخص‌های گلیسمیک، کلسترول تام، LDL-C و تری‌گلیسیرید در گروه تمرین هوازی در مقایسه با غذای پرچرب کاهش یافت(05/0>P). این مطالعه نشان داد که تمرینات هوازی حتی در زمان مصرف غذای پرچرب غیراشباع می‌تواند سطوح سرمی آدیپونکتین تام را در موش‌های چاق افزایش و همسو با آن موجب بهبود نیم‌رخ لیپیدی و متابولیکی شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of unsaturated-high-fat-diet and aerobic training program on serum levels of adiponectin isoforms, insulin resistance and lipid profile in male obese rats

نویسندگان [English]

  • payam saidie 1
  • hamid mohebbi 2
  • farhad Rahmani nia 3
  • Fahime Mohammad ghasemi 4
1 . Assistant Professor, Guilan University, Rasht, Iran
2 . Professor, Guilan University, Rasht, Iran
3 . Professor, Guilan University, Rasht, Iran
4 Histology Department, Medical science University, Guilan university, Rasht, Iran
چکیده [English]

The aim of present study was to investigate the effect of 10-week aerobic training and unsaturated-high-fat-diet on adiponectin serum in obese male rats. Blood samples were collected of forty-eight wistar male rats (average weight 195±5g, age 8 weeks) at 1st, 18th and 28th weeks after overnight fasting. After first sampling, remaining 40 rats were randomly divided into control (n=16) and high-fat-diet (39% of energy content from unsaturated-fat) (n=24). High-fat-diet group randomly divided in to exercise training and high-fat-diet subgroups after 18 weeks. Exercise training group ran for 10 weeks (5d/wk) with intensity equivalent to 70 to 75% of vo2max. Serum levels of total and HMW adiponectin as well as lipid profile and glycemic variables were measured. The results of this study indicate that in comparison to unsaturated-high-fat-diet, aerobic training in unsaturated-high-fat-diet induced obese male rats, increased serum concentration of total adiponectin significantly (P> 0/01). Also insulin resistance index and serum concentrations of insulin, glucose, total cholesterol, LDL-C and TG were decreased in aerobic exercise compare to high-fat-diet group (P> 0/05). This study indicates that aerobic training increase serum concentrations of total adiponectin in obese male rats even with simultaneous consumption of unsaturated-high-fat-diet and cause improvement in lipid and metabolic profiles.

کلیدواژه‌ها [English]

  • Adiponectin
  • insulin resistance
  • Aerobic training
  • Unsaturated-high-fat-diet
  • lipid profile

 

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