تاثیر کمکی (اجوانتی) تمرین ورزشی استقامتی کوتاه مدت بر فعال‌سازی سلول‌های T تنظیمی در واکسن HSV-1 و یادآورهای آن

نوع مقاله: مقاله پژوهشی

نویسندگان

1 .استادیار، دانشکدۀ علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

2 . دانشجوی کارشناسی ارشد، دانشکدة علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

3 گروه ایمونولوژی پزشکی انستیتو پاستور ایران، تهران، ایران

چکیده

هدف: تاثیر فعالیت ‌های ورزشی به صورت مزمن و حاد به عنوان ادجوانت واکسن در برخی از مطالعات بررسی شده است. هدف از پژوهش حاضر بررسی ادجوانتی یک دوره تمرین ورزشی استقامتی کوتاه مدت بر فعال‌سازی سلول‌های T تنظیمی در مدل واکسن HSV-1 بود. مواد و روش ها: 24 موش نژاد بالبسی در سه گروه کنترل، گروه واکسن و گروه دریافت کننده واکسن و تمرین ورزشی استقامتی کوتاه مدت تقسیم شدند. فعال سازی سلول‌های T تنظیمی پس از کشت سلولی طحال توسط تکنیک الایزا سنجیده شد. برای بررسی متغیرهای پژوهش از روش آماری آنالیز واریانس یک طرفه استفاده شد. یافته ها: نتایج پژوهش نشان داد بین گروه‌های پژوهش در مورد فعال سازی سلول‌های T تنظیمی تفاوت معناداری مشاهده می شود (P<0.05). نتایج آزمون پیگیری توکی نشان دهنده وجود تفاوت معنادار بین گروه های کنترل و واکسن با گروه دریافت کننده واکسن و تمرین ورزشی استقامتی کوتاه مدت است (P<0.05). نتیجه گیری: نتایج پژوهش حاضر نشان دهنده افزایش فعال سازی سلول‌های T تنظیمی به دنبال استفاده از یک دوره کوتاه مدت تمرین ورزشی به عنوان ادجوانت بوده است. این نتایج پیشنهاد می-کند استفاده از تمرین ورزشی استقامتی کوتاه مدت به عنوان ادجوانت ممکن است کارایی واکسن را افزایش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Adjuvant Effect of Short Term Endurance Training on Regulatory T Cells in HSV-1 Vaccine and Its Boosters

نویسندگان [English]

  • Mahdiyeh molanouri 1
  • Afsaneh Jamali Jamali 2
  • Mehdi Mahdavi 3
1 . Assistant Professor, Department of Physical Education and Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
2 MSc Student of Exercise Physiology, Tarbiat Modares University, Tehran, Iran y
3 Assistant Professor, Medical Immunology Department, Pasteur Institute of Iran, Tehran, Iran
چکیده [English]

Introduction: The herpes simplex viruses (HSV) cause a variety of clinical illnesses that are painful and often distressing. To control the infection, the development of an effective vaccine that prevents or reduces the primary and recurrent infections would be of great significance. Chronic and acute exercise training were used as a vaccine adjuvant in some studies. The purpose of this study was to assess adjuvant effects of short term endurance exercise training on regulatory T cells in HSV-1 vaccine model. Methods & Materials: 24 BALB/C mice were divide into 3 groups: control, vaccine and vaccine plus short term endurance exercise training. TGF-β levels as index of reglulatory T cells activation were determined in spleen cell culture with ELISA method. One-way ANOVA were used for statistical analysis. Results: The result of this study indicated that there are significant differences between group in reglulatory T cells activation (P<0.05). Tukey post-hoc test results showed significant differences between control and vaccine groups with vaccine plus short term endurance exercise training group (P<0.05). Conclusion: Results of current study showed that reglulatory T cells activation increased following using short term endurance exercise training as an adjuvant. These results suggested that coupling short term moderate exercise training with vaccination may enhance vaccine efficacy. Further studies are in order to determine effects of different kind of exercise as an adjuvant in different types of vaccine can be considerate.

کلیدواژه‌ها [English]

  • Adjuvant
  • Endurance training
  • Herpes Simplex viruses
  • Regulatory T Cells
1. Koelle DM. “Vaccines for herpes simplex virus infections”. Curr Opin Investig Drugs. 2006;7(2):136-41.

2. Whitley RJ, Roizman B (2002). “Herpes simplex viruses: is a vaccine tenable?” Journal of Clinical Investigation. 110(2):145-51.

3. Gallichan WS, Woolstencroft RN, Guarasci T, McCluskie MJ, Davis HL, Rosenthal KL (2001). “Intranasal immunization with CpG oligodeoxynucleotides as an adjuvant dramatically increases IgA and protection against herpes simplex virus-2 in the genital tract”. The Journal of Immunology. 166(5):3451-7.

4. Janeway CA Jr, Travers P, Walport M, et al (2001). “Immunobiology: The Immune System in Health and Disease”. 5th edition. New York: Garland Science.

5. Petrovsky N, Aguilar J.C (2004). “Vaccine adjuvants: Current state and future trends”. Immunology and Cell Biology; 82, 488–496.

6. Edwards KM, Burns VE, Ring C, Carroll D (2006). “Individual differences in the interleukin-6 response to maximal and submaximal exercise tasks”. J Sports Sci. 24(8):855-62.

7. Viswanathan K, Daugherty C, Dhabhar FS. “Stress as an endogenous adjuvant: augmentation of the immunization phase of cell-mediated immunity”. Int Immunol. 2005;17(8):1059-69.

8. Edwards KM, Burns VE, Adkins AE, Carroll D, Drayson M, Ring C (2008). “Meningococcal A vaccination response is enhanced by acute stress in men”. Psychosomatic medicine. 70(2):147-51.

9. Edwards KM, Burns VE, Allen LM, McPhee JS, Bosch JA, Carroll D et al (2007). “Eccentric exercise as an adjuvant to influenza vaccination in humans”.  Brain Behav Immun. 21(2):209-17.  

10. Long JE, Ring C, Drayson M, Bosch J, Campbell JP, Bhabra J et al (2012). “Vaccination response following aerobic exercise: can a brisk walk enhance antibody response to pneumococcal and influenza vaccinations?” Brain Behav Immun. 26(4):680-7.

11. Rogers CJ, Zaharoff DA, Hance KW, Perkins SN, Hursting SD, Schlom J, Greiner JW (2008). “Exercise enhances vaccine-induced antigen-specific T cell responses”. Vaccine. 3;26(42):5407-15.

12. Pascoe AR, Fiatarone Singh MA, Edwards KM (2014). “The effects of exercise on vaccination responses: a review of chronic and acute exercise interventions in humans”. Brain Behav Immun. 39:33-41.

13. Gleeson M. “Immune function in sport and exercise”. J Appl Physiol (1985). 2007;103(2):693-9.

14. McGeachy MJ, Bak-Jensen KS, Chen Y, Tato CM, Blumenschein W, McClanahan T et al (2007). “TGF-beta and IL-6 drive the production of IL-17 and IL-10 by T cells and restrain T(H)-17 cell-mediated pathology”. Nat Immunol. 2007;8(12):1390-7.

15. Letterio JJ, Roberts AB (1998). “Regulation of immune responses by TGF-beta”. Annu Rev Immunol. 16:137-61.

16. Davis JM, Kohut ML, Colbert LH, Jackson DA, Ghaffar A, Mayer EP (1997). “Exercise, alveolar macrophage function, and susceptibility to respiratory infection”. J Appl Physiol (1985). 83(5):1461-6.

17. Steensberg, A. Keller, C. Starkie, R.L. Osada, T. Febbraio, M.A. Pedersen, B.K. (2002).  “IL-6 and TNF-alpha expression in, release from, contracting human skeletal muscle”. Am J Physiol Endocrinol Metab 283: E1272–E1278.

18. Coffman RL, Sher A, Seder RA (2010). “Vaccine adjuvants: putting innate immunity to work. Immunity”. 29;33(4):492-503.

19. Gallucci S, Lolkema M, Matzinger P (1995). “Natural adjuvants: endogenous activators of dendritic cells”. Nature medicine. 5(11):1249-55.

20. Rock KL, Hearn A, Chen CJ, S1hi Y (2005). “Natural endogenous adjuvants”. Springer Semin Immunopathol. 26(3):231-46.

21. Woods JA, Vieira VJ, Keylock KT (2006). “Exercise, inflammation, and innate immunity”. Neurol Clin. 24(3):585-99.

22. Edwards KM, Campbell JP, Ring C, Drayson MT, Bosch JA, Downes C, et al (2010). “Exercise intensity does not influence the efficacy of eccentric exercise as a behavioural adjuvant to vaccination”. Brain, behavior, and immunity. 24(4):623-30.

23. Molanouri Shamsi M, Hassan ZM, Quinn LS, Gharakhanlou R, Baghersad L, Mahdavi M (2015). “Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype”. Endocrine. 49(2):396-403.

24. Molanouri Shamsi M, Mahdavi M, Quinn LS, Gharakhanlou R, Isanegad A (2016). “Effect of resistance exercise training on expression of Hsp70 and inflammatory cytokines in skeletal muscle and adipose tissue of STZ-induced diabetic rats”. Cell Stress Chaperones. 2016;21(5):783-789.

25. Pedersen, BK. Febbraio, M.A. (2008). “Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6”. Physiol. Rev. 88: 1379-1406.

26. Pedersen, BK. Fischer, CP (2007). “Physiological roles of muscle-derived interleukin-6 in response to exercise”. Curr Opin Clin Nutr Metab Care 10: 265–271.

27. Fischer CP (2006). “Interleukin-6 in acute exercise and training: what is the biological relevance?” Exerc Immunol Rev. 12:6-33.