تاثیر چهارده هفته فعالیت هوازی همزمان با مکمل‌دهی رزوراترول روی میزان پروتئین SIRT1 و UCP-1 و PGC-1α در بافت کبد ی، چربی زیر پوستی و چربی احشایی موش های صحرایی نر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بیوشیمی‌ و متابولیسم ورزشی، دانشگاه آزاد تهران مرکز

2 گروه فیزیوبوژی، دانشکده تربیت بدنی دانشگاه آزاد تهران مرکزی

3 . دانشیار پژوهشکدۀ علوم غدد و متابولیسم، دانشگاه علوم پزشکی شهید بهشتی تهران –تهران-ایران

چکیده

هدف از تحقیق حاضر تعیین تاثیر مکمل‌دهی رزوراترول و فعالیت هوازی بر میزان پروتئین SIRT-1 ، PGC-1αو UCP-1 بافت کبدی و بافت چربی شکمی‌-کشاله‌ای و احشایی در موش‌های صحرایی نر بود. در این پژوهش 28 سر موش صحرایی (میانگین وزن10 ±260 گرم، سن 8 هفته)، به طور تصادفی به چهار گروه؛ شاهد (C)، تمرین (T)، مکمل-تمرین (T-S) و مکمل (S) تقسیم شدند. گروه‌های تمرینی به مدت 14 هفته (‌هفته ای 5 جلسه، هر جلسه به مدت 45دقیقه) روی نوارگردان فعالیت کردند. گروه مکمل-تمرین روزانه 10میلی‌گرم به ازای هر کیلوگرم از وزن بدنشان مکمل رزوراترول دریافت کردند. جهت اندازه‌گیری پروتئین بافتی UCP-1، SIRT1، PGC-1α از روش الایزا (ساندویچ دوتایی) استفاده‌گردید. آزمون تحلیل واریانس یک‌طرفه برای تحلیل داده‌ها استفاده و سطح معنی‌داری (p≤0.05) در نظرگرفته‌شد. میزان تغییرات پروتئین SIRT1 و PGC-1α در بافت کبدی و چربی سفید زیرپوستی و احشایی به ترتیب (P≤0.05) و (P≤0.001) مشاهده شد. پروتئین UCP-1 نیز در بافت های مورد اندازه گیری در گروه مکمل-تمرین افزایش معنی داری را پس از مصرف مکمل همراه با فعالیت هوازی از خود نشان داد (P≤0.001). با توجه به نتایج بدست آمده احتمالا مکمل‌دهی موجب بهبود عملکرد بافت کبدی و تغییر فنوتیپ چربی سفید زیر پوستی به چربی بژ (بینابینی) می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of 14 weeks aerobic exercise on resveratrol supplementation on protein UCP-1, SIRT1, PGC-1α in liver tissue, subcutaneous and visceral fat tissue in male wistar rats

نویسندگان [English]

  • hadi zahedi 1
  • mehdi hedayati 3
1 .PhD Student of Sport Biochemistry and Metabolism, Islamic Azad University, Central Tehran Branch
3 Associate Professor of Endocrinology and Metabolism Research Institute, Shahid Beheshti University of Medical Sciences, Tehran
چکیده [English]

The purpose of this study was to determine the effects of resveratrol supplementation and Aerobic training on protein levels of SIRT-1, PGC-1α and UCP-1 liver tissue and abdominal & inguinal subcutaneous adipose tissue in adult male Wistar rats. In this study, 28 male rat (w=260+10gr, 8 weeks) divided into 4 (n=8) groups: control (C), aerobic training (T), complement-training (T-R) and supplements (S) randomly. Training groups exercised for 14 weeks (5 sessions a week, each session 45 minutes on a treadmill. ELISA (sandwich double) was used to measure tissue proteins UCP-1, SIRT1, PGC-1α. ANOVA test used for data analysis and statistical significance (p≤0.05) were considered. SIRT1 and PGC-1α protein was observed changes in liver tissue and subcutaneous / visceral white adipose tissue, respectively (P≤0.05) and (P≤0.001). Increased protein UCP-1 is also measured in tissues-workout supplement after supplement in combination with aerobic activity (P≤0.001). According to our results, are likely to impact more powerful, resveratrol supplementation with aerobic physical activity increases the amount of UCP-1, SIRT1, PGC-1α tissue to separate than from physical activity or resveratrol supplements, And is likely to Improved liver function and change subcutaneous white adipose tissue to beige or intermediate phenotype.

کلیدواژه‌ها [English]

  • UCP-1
  • SIRT1
  • PGC-1α
  • Aerobic training
  • Resveratrol

 

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