مقایسۀ تأثیر یک جلسه فعالیت ورزشی تداومی و تناوبی با شدت بالا بر سطوح TNF-α و VEGF بافت عضلۀ نعلی موش‌های چاق

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس‌ارشد دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه صنعتی شاهرود، شاهرود، سمنان، ایران

2 دانشیار، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه صنعتی شاهرود، شاهرود، سمنان، ایران

3 دانشیار، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه شهید بهشتی، تهران، ایران

چکیده

هدف پژوهش حاضر مقایسۀ اثر یک جلسه فعالیت ورزشی تداومی و تناوبی با شدت بالا بر سطوح  TNF-αو  VEGF بافت عضلۀ نعلی موش­های چاق بود. به این منظور 45 سر موش هشت‌هفته‌ای به مدت هشت هفته با رژیم پر­چرب تغذیه شدند. سپس 27 سر موش­ با شاخص تودۀ بدنی بیش از gr/cm2  68/0 به وزن gr10±320 به‌طور تصادفی به سه گروه کنترل، فعالیت تداومی و فعالیت تناوبی با شدت بالا تقسیم شدند. پس از دو هفته آشناسازی و تعیین حداکثر اکسیژن مصرفی، گروه تناوبی و تداومی به‌ترتیب با شدت 100 درصد vVO2maxو 65 درصد vVO2maxدویدند، به‌طوری‌که مدت زمان دویدن براساس مسافت در گروه فعالیت ورزشی تناوبی محاسبه شد. گروه کنترل در هیچ
برنامه­ای شرکت نکردند. بلافاصله حیوانات تشریح و عضلۀ نعلی آنها جدا شد. پس از تعیین نرمال بودن داده­ها از آزمون­ آماری واریانس یکطرفه استفاده شد(05/0P<). یافته­ها نشان داد فقط گروه فعالیت ورزشی تناوبی در مقایسه با گروه کنترل (001/0=P) و با گروه تداومی (003/0=P) موجب کاهش معنا­دار سطوح VEGF شد، اما سطوح  TNF-αدر هر سه گروه تفاوت معنا­داری نداشت. همچنین نتایج نشان داد که برخلاف تصورات قبلی، فعالیت ورزشی تناوبی شدید موجب افزایش التهاب نشد. نگرانی در مورد انجام فعالیت ورزشی تناوبی و بالا رفتن التهاب و همچنین کاهش سطوح VEGF گروه تناوبی در افراد چاق مورد تردید است که تحقیقات بیشتری در این زمینه مورد نیاز است

کلیدواژه‌ها


عنوان مقاله [English]

A Comparison of the Effect of One Session of Continuous and High Intensity Intermittent Training on TNF-α and VEGF Levels of Soleus Tissue in Obese Rats

نویسندگان [English]

  • maryam baghery 1
  • ali yunesian 2
  • Rahimeh Mehdizadeh 2
  • Maryam Nourshahi 3
1 .MSc, Faculty of Physical Education and Sport Sciences, Shahrood University of Technology, Shahrood, Semnan, Iran
2 Associate Professor, Faculty of Physical Education and Sport sciences, Shahrood University of Technology, Shahrood, Semnan, Iran
3 Associate Professor, Faculty of Physical Education and Sport Sciences, Shahid Beheshti University, Tehran, Iran
چکیده [English]

The aim of this study was to compare the effect of one session of continuous and high intensity intermittent training on TNF-α and VEGF levels of soleus tissue in obese rats. 45 rats (8 weeks old) were fed a high fat diet for 8 weeks. Then, 27 rats (BMI more than 0.68 gr/cm2 and weight: 320±10 gr) were randomly divided into 3 groups: control, continuous and high intensity intermittent training. Intermittent and continuous groups were familiarized with the study and their VO2max was determined for 2 weeks; then, they ran respectively with an intensity of 100% vVO2max and 65% vVO2max and the running time was calculated based on distance in the intermittent training group. Control group did not participate in any programs. Animals were dissected immediately and their soleus muscle was removed. The normality of the data was determined and one-way ANOVA was used (P≤0.05). Only the intermittent training group significantly decreased VEGF levels in comparison with the control group (P=0.001) and the continuous group (P=0.003). But TNF-α levels were not significantly different in all 3 groups. Contrary to previous speculations, high intensity intermittent training did not ascend inflammation. There is a doubt about the concern for intermittent training and inflammation increase and also VEGF levels decrease in the intermittent group in obese people which requires more research in this field.
 

کلیدواژه‌ها [English]

  • Continuous training
  • high intensity intermittent training
  • TNF-α
  • VEGF

1. Waterston A, Bower M. TNF and cancer: good or bad. Cancer therapy. 2004;2:131-48.

2.   Yaghoubi M, Esmailzadeh H, Yaghoubi G. Relationship between Physical Activity and Prevalence of Obesity and Overweight in the Disabled and Veterans. J Mil Med. 2013;14(4):245-8.

3.   Mohammadi N, Shobeiri F, Khirollahi A. Frequency of Over Wieght & Obesity of Women in Referents to Medical and Health Centers in Hamadam City. Scientific Journal of Hamadan Nursing & Midwifery Faculty. 2011;19(2):36-46.

4.   Organization WH. Obesity: preventing and managing the global epidemic: report of a WHO consultation. WHO Technical Report. 1999;894.

5.   Olfert IM, Howlett RA, Wagner PD, Breen EC. Myocyte vascular endothelial growth factor is required for exercise-induced skeletal muscle angiogenesis. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2010;299(4):R1059-R67.

6.   Ogunbode A, Ladipo M, Ajayi I, Fatiregun A. Obesity: an emerging disease. Nigerian journal of clinical practice. 2011;14(4):390-4.

7.   Kazemi A, Rahmati M, Eskandari F, Taherabadi S. Effect of 8 weeks sprint interval training on serum levels of Adiponectin and insulin in overweight children. ISMJ. 2016;19(1):37-47.

8.   Christiansen T, Bruun JM, Paulsen SK, Ølholm J, Overgaard K, Pedersen SB, et al. Acute exercise increases circulating inflammatory markers in overweight and obese compared with lean subjects. European journal of applied physiology. 2013;113(6):1635-42.

9.   Kern PA, Ranganathan S, Li C, Wood L, Ranganathan G. Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance. American Journal of Physiology-Endocrinology And Metabolism. 2001;280(5):E745-E51.

10. Tsai M-S, Kuo M-L, Chang C-C, Wu Y-T. The effects of exercise training on levels of vascular endothelial growth factor in tumor-bearing mice. Cancer Biomarkers. 2013;13(5):307-13.

11. Suhr F, Brixius K, de Marées M, Bölck B, Kleinöder H, Achtzehn S, et al. Effects of short-term vibration and hypoxia during high-intensity cycling exercise on circulating levels of angiogenic regulators in humans. Journal of applied physiology. 2007;103(2):474-83.

12. Lundby C, Calbet JA, Robach P. The response of human skeletal muscle tissue to hypoxia. Cellular and molecular life sciences. 2009;66(22):3615-23.

13. Grunewald M, Avraham I, Dor Y, Bachar-Lustig E, Itin A, Yung S, et al. VEGF-induced adult neovascularization: recruitment, retention, and role of accessory cells. Cell. 2006;124(1):175-89.

14. Hellsten Y, Rufener N, Nielsen JJ, Høier B, Krustrup P, Bangsbo J. Passive leg movement enhances interstitial VEGF protein, endothelial cell proliferation, and eNOS mRNA content in human skeletal muscle. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2008;294(3):R975-R82.

15. Hoier B, Passos M, Bangsbo J, Hellsten Y. Intense intermittent exercise provides weak stimulus for vascular endothelial growth factor secretion and capillary growth in skeletal muscle. Experimental physiology. 2013;98(2):585-97.

16. Kazemi A, Eslami R, Karimqasemi L. The Effect of High Intensity Interval Training on Tumor Necrosis Factor-alpha Levels in Visceral and Subcutaneous Adipose Tissue and Insulin Resistance in Male Rats. Sport Physiology. 2017;8 (32):17-30.

17. Gokhale R, Chandrashekara S, Vasanthakumar K. Cytokine response to strenuous exercise in athletes and non-athletes—an adaptive response. Cytokine. 2007;40(2):123-7.

18. Fritzenwanger M, Jung C, Goebel B, Lauten A, Figulla HR. Impact of short-term systemic hypoxia on phagocytosis, cytokine production, and transcription factor activation in peripheral blood cells. Mediators of inflammation. 2011;2011.

19. Ravasi AA, Yadegari M, Choobineh S. Comparison of two types of physical activity on response serum VEGF-A, non-athletic men. Journal of Biological Sciences. 2014;6:56-41.

20. Nourshahi M, Feizemilani R, Gholamali M. Effects of acute eccentric exercise on serum vascular endothelial growth factor and endostatin concentration in male wistar rats. Journal of Sport in Biomotor Sciences. 2012;5(1):86-94.

21. Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, MacDonald MJ, McGee SL, et al. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. The Journal of physiology. 2008;586(1):151-60.

22. MacLaren D, Morton J. Biochemistry for sport and exercise metabolism: John Wiley & Sons; 2011.

23. Molanouri Shamsi M, Mahdavi M, Gharakhanlou R, Zahir MH. Effect of Resistance Training on Protein Expression of IL6, IL-1β and TNF-α Myokines in Fast Twitch Skeletal Muscle of Diabetic Rats. Journal of Sport in Biomotor Sciences. 2013;11(1):69-77.

24. Batista Jr M, Rosa J, Lopes R, Lira F, Martins Jr E, Yamashita A, et al. Exercise training changes IL-10/TNF-α ratio in the skeletal muscle of post-MI rats. Cytokine. 2010;49(1):102-8.

25. Lira FS, Rosa JC, Yamashita AS, Koyama CH, Batista Jr ML, Seelaender M. Endurance training induces depot-specific changes in IL-10/TNF-α ratio in rat adipose tissue. Cytokine. 2009;45(2):80-5.

26. Cheraghpour M, Ehrampoush E, Homayounfar R, Davoodi H, Zand H, Mimmiran P. The relationship between the immune system and the inflammatory mechanisms in obesity with insulin resistance. Iranian Journal of Nutrition Sciences & Food Technology. 2013;7(5):723-35