تأثیر 14 هفته فعالیت استقامتی بر بیان miR-1 بطن چپ رت‌های نر نژاد ویستار

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار فیزیولوژی ورزش گروه تربیت بدنی، دانشگاه لرستان، خر مآباد، ایران

2 دانشیار، گروه تربیت بدنی، دانشکدة علوم انسانی دانشگاه تربیت مدرس، تهران، ایران

3 دانشیار، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، کرج، ایران

چکیده

miR-1 در بسیاری از فرایندهای سلولی از جمله هایپرتروفی بافت عضلانی (اسکلتی و قلبی) درگیر است. فعالیت‌های استقامتی نیز موجب هایپرتروفی قلب می‌شود، بنابراین هدف این مطالعه بررسی اثر فعالیت استقامتی بر بیان miR-1 قلب است. بدین منظور 14 رت در شرایط کنترل‌شده (دما، چرخۀ روشنایی و تاریکی و دسترسی آزاد به آب و غذا) نگهداری شدند و بعد از آشناسازی با پروتکل تمرینی به‌صورت تصادفی در دو گروه کنترل و تجربی قرار گرفتند. گروه تجربی یک برنامۀ (14 هفته‌ای) استقامتی را روی تردمیل اجرا کرد. در ادامه 48 ساعت پس از پایان آخرین جلسۀ تمرینی بی‌هوش و تشریح شدند، سپس قلب و بطن چپ آنها خارج و با استفاده از روش Real time-PCR  میزان بیان miR-1 قلب آنها اندازه‌گیری شد. در پایان با استفاده از آزمون آماری t اطلاعات به‌دست‌آمده ارزیابی شد. نتایج نشان داد که تفاوت معناداری در میانگین بیان miR-1 قلب گروه کنترل و تجربی وجود ندارد (191.P=). فعالیت استقامتی بر بیان miR-1 قلب تأثیر معناداری نداشت، احتمال دارد تغییرات miR-1 هرچند کم، در سطح پروتئین ژن‌های هدف آن مشخص شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 14 Week of Endurance Activity on miR-1 Expression of Left Ventricle in Male Wistar Rats

نویسندگان [English]

  • Mohammad Fathi 1
  • Reza Gharakhanlou 2
  • Hamid Rajabi 3
چکیده [English]

miR-1 is involved in many cellular processes including muscle hypertrophy (skeletal and cardiac). Endurance activities also cause cardiac hypertrophy. Therefore, the aim of this study was to investigate the effect of endurance activity on cardiac expression of miR-1. For this purpose, 14 rats were maintained under controlled conditions (temperature, light/dark (12:12) cycle, with ad libitum access to food and water) and randomly assigned to control and experimental groups after they were familiarized with the training protocol. The experimental group performed an (14 weeks) endurance training program on a treadmill; then, 48 hours after the end of the last session, they were anesthetized and sacrificed and their heart and left ventricle were removed. To determine the cardiac miR-1 expression, Real time-PCR method was used. The obtained data were evaluated using t test. Results showed no significant differences in the mean of cardiac miR-1 expression between the two groups (P =0.191). The endurance activity had no significant effects on cardiac miR-1 expression. It is likely that changes in miR-1, although low, appear in proteins level of target genes.

کلیدواژه‌ها [English]

  • endurance activity
  • Heart
  • left ventricle
  • miR-1

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