نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دکتری فیزیولوژی ورزشی، دانشکدۀ علوم انسانی، دانشگاه آزاد اسلامی واحد خمینی‌شهر، خمینی‌شهر، اصفهان، ایران
2 استاد فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، گروه فیزیولوژی ورزش، دانشگاه گیلان، رشت، ایران
3 استاد فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، گروه فیزیولوژی ورزش، دانشگاه گیلان، رشت، ایرا
4 استادیار فیزیولوژی حیوانی، دانشکدۀ کشاورزی، گروه دامپروری، دانشگاه صنعتی اصفهان، اصفهان، ایران
چکیده
هدف از پژوهش حاضر، بررسی آثار تمرین تناوبی بر سطح لاکتات و فعالیت آنزیم لاکتات دهیدروژناز خون موش‌های صحرایی بود. 20 سر موش صحرایی نر نژاد ویستار (میانگین سن و وزن به‌ترتیب 3 ماه و 14± 224 گرم) تهیه شده و سپس، به‌طور تصادفی به دو گروه شاهد (10 = n) و تجربی (10 = n) تقسیم شدند. پروتکل تمرینی شامل 4 دقیقه دویدن روی نوار گردان و 2 دقیقه استراحت فعال در 10 مرحلۀ تمرینی روی گروه تجربی اجرا شد. 48 ساعت بعد از آخرین جلسۀ تمرینی بعد از ناشتایی شبانه، موش‌ها با ترکیبی از کتامین و زایلازین بیهوش شدند. برای اندازه‌گیری لاکتات و فعالیت آنزیم LDH، خون‌گیری از قلب حیوان انجام گرفت. اطلاعات با استفاده از شاخص‌های پراکندگی میانگین و انحراف معیار (SE ±M) و آزمون t مستقل تجزیه‌وتحلیل شد. نتایج نشان داد که تفاوت معناداری در سطح لاکتات خون دو گروه وجود نداشت، ولی در میزان فعالیت آنزیم LDH، تفاوت معناداری بین دو گروه تجربی و شاهد مشاهده شد (05/0P <). این نتایج نشان می‌‌دهد، تمرین تناوبی به پاکسازی لاکتات منجر میشود. پایش لاکتات، موجب حفظ گلیکوژن عضلات شده و از تجمع +H که همراه با لاکتات تولید میشود، جلوگیری می‌‌کند.
کلیدواژه‌ها
عنوان مقاله [English]
The Effects of 8 Weeks of Intermittent Training on Lactate (La) Level and Lactate Dehydrogenase (LDH) Enzyme Activity in Male Wistar Rats
نویسندگان [English]
- Allahyar Arabmomeni 1
- Hamid Mohebi 2
- Farhad Rahmani nia 3
- Ahmad Riasi 4
1 PhD of Exercise Physiology, Faculty of Human Sciences, Islamic Azad University, Khomeinishahr Branch, Khomeinishahr, Isfahan, Iran
2 Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
3 Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
4 Assistant Professor of Animal Physiology, Faculty of Agriculture, Industrial University of Isfahan, Isfahan, Iran
چکیده [English]
The aim of this study was to assess the effects of intermittent training on lactate level and lactate dehydrogenase enzyme activity in Wistar rats. 20 male Wistar rats (mean age 3 months and weight 224±14 g) were selected and randomly divided into the training (n=10) and control (n=10) groups. The training protocol consisted of running on a treadmill for 4 minutes and then 2 minutes of active rest in 10 training phases for the experimental group. All rats were anesthetized with a mixture of ketamine and xylazine 48 hours after the last training session after an overnight fasting. To measure lactate and LDH enzyme activity, blood samples were obtained from their cardiac puncture. Data were analyzed by mean and standard deviation (M+SD) and independent t test. The results showed no significant differences in blood lactate level between the two groups, but there was a significant difference in LDH enzyme activity between the two groups (P˂0.05). These results indicate that intermittent training caused the clearance of lactate. Enhance of lactate replenished muscle glycogen and prevented H+ concentration which was produced along with lactate.
کلیدواژه‌ها [English]
- intermittent training
- lactate (La)
- lactate dehydrogenase (LDH) enzyme
- rat
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