نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزش، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران،
2 دانشیار گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران
3 استاد گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران،
4 استادگروه فیزیولوژی ورزشی، دانشکده علوم ورزشی و تندرستی، دانشگاه تهران، تهران، ایران،
چکیده
مقدمه: مالتیپل اسکلروزیس (MS) یک بیماری التهابی خودایمنی است که بر عملکرد شناختی در طول توسعۀ بیماری اثر دارد. یکی از تغییرات مهم در سیستم اعصاب مرکزی این بیماران اختلال سیناپسی است که پیش از دمیلینه شدن اکسونها و مسیرهای عصبی رخ میدهد. پژوهشهای پیشین نشاندهندۀ این نکته است که فعالیت بدنی در پیشگیری و درمان چنین شرایطی امیدوارکننده است. تحقیق حاضر با هدف بررسی تغییرات حاصل از ورزش شنا بر پروتئین سیناپتوزومی SNAP25 بافت مغز دخیل در اختلالات شناختی و التهابی MS انجام شد.
روش پژوهش: از 21 موش سوری C57BL/6 در سه گروه به تعداد مساوی کنترل، آنسفالومیلیت خودایمنی تجربی بدون تمرین و با چهار هفته شنای هوازی استفاده شد. نمرات بالینی روزانه و سطح پروتئین SNAP25 پس از چهار هفته تمرین با روش وسترن بلات اندازهگیری شد.
یافتهها: القای EAE سطح SNAP25 را نسبت به گروه کنترل سالم افزایش داد، اما ورزش شنا توانست آن را .۲۷ درصد کاهش دهد نمرات بالینی نیز در پی انجام ورزش در موشهای بیمار بهبود یافت.
نتیجهگیری: ورزش منظم روزانه با تنظیم بیان SNAP25 در بافت مغز، احتمالاً رهایش واسطههای عصبی و عوامل تروفیک در ناحیۀ سیناپس را در شرایط التهابی تعدیل میکند و موجب بهبود علائم اختلال شناختی میشود.
کلیدواژهها
موضوعات
عنوان مقاله [English]
The Effect of Aerobic Swimming on Synaptic Protein Expression and Improvement of Cognitive Function in Multiple Sclerosis Mice Model
نویسندگان [English]
- Soheila Banasadegh 1
- Shahnaz Shahrbanian 2
- Reza Ghara khanlou 3
- Mohammadreza Kordi 4
1 Department of Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
2 Department of Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
3 Department of Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
4 Department of Sports Physiology, Faculty of Health and Sports Sciences, University of Tehran, Tehran, Iran.
چکیده [English]
Introduction: Multiple sclerosis (MS) is an autoimmune inflammatory disease of the CNS that impairs cognitive function during its progression. A notable alteration in the CNS of these patients is synaptic dysfunction, which precedes axonal and neural pathway demyelination. Previous research shows that physical activity is promising potential in the prevention and management of these conditions. This study aimed to examine the effects of swimming exercise on the levels of the presynaptic membrane protein SNAP25 in the brain tissue of female mice experiencing cognitive and inflammatory disorders, using the experimental autoimmune encephalomyelitis (EAE) animal model.
Methods: A total of 21 C57BL/6 mice were divided into 3 groups with equal numbers: control, EAE without exercise, and EAE with aerobic swimming exercise. Clinical scores were recorded daily, and SNAP25 protein levels were measured by Western blot method after the 4-week exercise period.
Results: The induction of EAE increased SNAP25 levels compared to the healthy control group, but swimming exercise reduced these levels by 27% (P-value=0.0003, F=42.92, EF=0.85). Additionally, clinical scores improved in the exercised mice with the disease.
Conclusion: Aerobic swimming for four weeks by regulating the expression of SNAP25 in the brain tissue and modulating the release of neural mediators and nutritional factors in the synaptic area improves the symptoms of mild cognitive impairment and clinical symptoms in mice model of MS.
Methods: A total of 21 C57BL/6 mice were divided into 3 groups with equal numbers: control, EAE without exercise, and EAE with aerobic swimming exercise. Clinical scores were recorded daily, and SNAP25 protein levels were measured by Western blot method after the 4-week exercise period.
Results: The induction of EAE increased SNAP25 levels compared to the healthy control group, but swimming exercise reduced these levels by 27% (P-value=0.0003, F=42.92, EF=0.85). Additionally, clinical scores improved in the exercised mice with the disease.
Conclusion: Aerobic swimming for four weeks by regulating the expression of SNAP25 in the brain tissue and modulating the release of neural mediators and nutritional factors in the synaptic area improves the symptoms of mild cognitive impairment and clinical symptoms in mice model of MS.
کلیدواژهها [English]
- EAE Induction
- Synaptic Destruction
- SNAP25
- Mild Cognitive Impairment
- Aerobic Exercise
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