اثر تعاملی شش هفته مکمل دهی بربرین به همراه تمرین هوازی بر برخی از شاخص های اکسایشی و ضداکسایشی و بیان ژن کاسپاز-3 در عضلۀ چهارسرران موش های صحرایی نر دیابتی شده با استرپتوزوسین

حمزه زاده, اعظم; رمضانی, جواد; نقیبی, سعید

doi:10.22059/jsb.2023.363750.1602

نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I

نویسندگان

¹ گروه فیزیولوژی فعالیت بدنی و تندرستی، دانشگاه پیام نور، مرکز کرج، ایران.

² نویسنده مسئول، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه پیام نور، تهران، ایران.

³ گروه علوم ورزشی، دانشکدة علوم انسانی، دانشگاه پیام نور، تهران، ایران.

https://doi.org/10.22059/jsb.2023.363750.1602

چکیده

مقدمه: دیابت نوع1 با ضعف دفاع آنتی‌اکسیدانی بدن همراه بوده و بر عضلات اسکلتی نیز تاثیر دارد. هدف از انجام این پژوهش، بررسی تعامل مکمل‌دهی بربرین به همراه تمرین‌هوازی بر مالون‌دی‌آلدئید، سوپراکسیددیسموتاز، گلوتاتیون‌پراکسیداز و همچنین بیان ژن Caspase-3 در بافت عضله چهارسر ران موش‌های صحرایی دیابتی نوع1 بود.

روش پژوهش: 35 سر موش‌‌صحرایی نر نژاد ویستار، بصورت تصادفی در پنج گروه 1.کنترل سالم، 2.کنترل دیابتی، 3.مکمل‌بربرین، 4.تمرین‌هوازی و 5.تمرین هوازی+مکمل‌بربرین قرار گرفتند. پس از اطمینان از القای دیابت توسط STZ در گروه‌های تیمار، تمرین‌هوازی با شدت متوسط به مدت شش هفته با تواتر پنج جلسه در هفته طبق برنامه انجام شد. مکمل‌دهی‌‌بربرین با دوز 50 mg/kg در تمام روزهای هفته و نیم ساعت قبل از تمرین به صورت گاواژ انجام شد.

یافته‌ها: سطح MDA و SOD در گروه مکمل‌بربرین+تمرین‌هوازی نسبت به سایر گروه‌ها به ترتیب کاهش(0019/0p<) و افزایش معنی‌دار(0063/0p<) داشت. سطح GPXدر گروه مکمل‌بربرین+تمرین‌هوازی نسبت به گروه‌های کنترل دیابت و تمرین‌هوازی(به ترتیب 0005/0p< و001/0p<) دارای افزایش معنی‌داری بود. همچنین در گروه مکمل‌بربرین نسبت به گروه کنترل دیابت(0017/0p<) افزایش معنی‌دار بود. با این حال تفاوت معنی‌داری بین گروه مکمل‌بربرین+تمرین‌هوازی و مکمل‌بربرین دیده نشد(132/0p=). بیان ژن Caspase-3 در گروه مکمل-بربرین+تمرین‌هوازی نسبت به گروه‌های کنترل دیابت و تمرین‌هوازی(به ترتیب0001/0p< و001/0p<) کاهش معنی‌داری داشت. این متغیر نیز در گروه مکمل‌بربرین نسبت به گروه کنترل دیابت کاهش معنی‌داری داشت(0005/0p<).

نتیجه‌گیری: به‌نظر می‌رسد، مداخلۀ همزمان مکمل‌‌دهی‌بربرین و تمرین‌هوازی دارای اثرات هم‌افزایی مثبت بوده و نقش مهمی در کاهش MDA و بیان ژن کاسپاز-3 و افزایش SOD و GPX در عضله چهارسرران موش‌های دیابتی نوع 1 دارد.

کلیدواژه‌ها

موضوعات

فیزیولوژی ورزشی

عنوان مقاله [English]

The Interactive Effect of Six Weeks of Berberine Supplementation with Aerobic Exercise on Some Oxidative and Anti-Oxidative Indices and Caspase-3 Gene Expression in the Quadriceps Muscle of Streptozotocin-Diabetic Male Rats

نویسندگان [English]

Azam Hamzezadeh ¹
Javad Ramezani ²
Saeid Naghibi ³

¹ Department of Sports Sciences, Faculty of Humanities, Payame Noor University, Karaj Center.

² Corresponding Author, Department of Sports Sciences, Faculty of Humanities, Payame Noor University, Tehran, Iran.

³ Department of Sports Sciences, Faculty of Humanities, Payame Noor University, Tehran, Iran.

چکیده [English]

Introduction: Type-1 diabetes is associated with the weakness of the body's antioxidant defense and also affects skeletal muscles. This research aimed to investigate the interaction of Berberine supplementation with aerobic exercise on Malondialdehyde (MDA), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPX), and also the Caspase-3 gene expression in quadriceps muscle tissue of type-1 diabetic rats.
Methods: 35 male Wistar rats were randomly divided into five groups: 1) healthy control, 2) diabetic control, 3) Berberine supplement, 4) aerobic exercise and 5) Aerobic exercise + Berberine supplement. After ensuring the induction of diabetes by STZ in the treatment groups, moderate intensity aerobic exercise was performed for six weeks with a frequency of five sessions per week according to the schedule. Berberine supplementation with a dose of 50 mg/kg was implemented by gavage on all days of the week and half an hour before exercise.
Results: The MDA and SOD levels in Berberine + aerobic exercise group significantly decreased (P<0.0019) and increased (P<0.0063), respectively compared to other groups. The level of GPX in the Berberine + aerobic exercise group had a significant increase compared to the diabetes control and aerobic exercise groups (P<0.0005 and P<0.001, respectively). Also, there was a significant increase in GPX level in the Berberine supplement group compared to the diabetes control group (P<0.0017), however, there was no significant difference between the group of Berberine + aerobic exercise and Berberine supplement (P=0.132). The Caspase-3 gene expression in Berberine + aerobic exercise group had a significant decrease compared to diabetes control and aerobic exercise groups (P<0.0001 and P<0.001, respectively). This variable also had a significant decrease in the Berberine supplement group compared to the diabetes control group (P<0.0005).
Conclusion: It seems that the simultaneous intervention of Berberine supplementation and aerobic exercise has positive synergistic effects and plays an important role in reducing MDA, caspase-3 gene expression, and increasing SOD and GPX in the quadriceps muscle of type-1 diabetic rats.

کلیدواژه‌ها [English]

Aerobic Exercise
Berberine
Caspase-3
Diabetic
Oxidative
Streptozotocin

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مراجع

مراجع

1] Anderson J, Couper JJ, Mpundu-Kaambwa C, Giles LC, Gent R, Coppin B, et al. An extra 1,000 steps per day relates to improved cardiovascular health in children with type 1 diabetes. Diabetes Care. 2016;39(8):e108–9. https://doi.org/10.2337/dc16-0526

[2] Vazeou A, Papadopoulou A, Miha M, Drakatos A, Georgacopoulos D. Cardiovascular impairment in children, adolescents, and young adults with type 1 diabetes mellitus (T1DM). Eur J Pediatr. 2008;167:877–84. https://doi.org/10.1007/s00431-007-0603-z

[3] Krause MP, Riddell MC, Hawke TJ. Effects of type 1 diabetes mellitus on skeletal muscle: clinical observations and physiological mechanisms. Pediatr Diabetes. 2011;12(4pt1):345–64. https://doi.org/10.1111/j.1399-5448.2010.00699.x

[4] Mehta MM, Weinberg SE, Chandel NS. Mitochondrial control of immunity: beyond ATP. Nat Rev Immunol. 2017;17(10):608–20. https://www.nature.com/articles/nri.2017.66

[5] Sifuentes-Franco S, Pacheco-Moisés FP, Rodríguez-Carrizalez AD, Miranda-Díaz AG. The role of oxidative stress, mitochondrial function, and autophagy in diabetic polyneuropathy. J Diabetes Res. 2017;2017. https://doi.org/10.1155/2017/1673081

[6] Scarpulla RC. Nucleus-encoded regulators of mitochondrial function: integration of respiratory chain expression, nutrient sensing and metabolic stress. Biochim Biophys Acta (BBA)-Gene Regul Mech. 2012;1819(9–10):1088–97. https://doi.org/10.1016/j.bbagrm.2011.10.011

[7] Ibuki FK, Bergamaschi CT, da Silva Pedrosa M, Nogueira FN. Effect of vitamin C and E on oxidative stress and antioxidant system in the salivary glands of STZ-induced diabetic rats. Arch Oral Biol. 2020;116:104765. https://doi.org/10.1016/j.archoralbio.2020.104765

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[9] Sala D, Zorzano A. Differential control of muscle mass in type 1 and type 2 diabetes mellitus. Cell Mol life Sci. 2015;72:3803–17. https://doi.org/10.1007/s00018-015-1954-7

[10] Tang L, Li N, Jian W, Kang Y, Yin B, Sun S, et al. Low-intensity pulsed ultrasound prevents muscle atrophy induced by type 1 diabetes in rats. Skelet Muscle. 2017;7:1–10. https://doi.org/10.1186/s13395-017-0145-7

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[20] Møller P, Wallin H, Knudsen LE. Oxidative stress associated with exercise, psychological stress and life-style factors. Chem Biol Interact. 1996;102(1):17–36. https://doi.org/10.1016/0009-2797(96)03729-5

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دوره 15، شماره 4دی 1402صفحه 85-100

[21] Golbidi S, Badran M, Laher I. Antioxidant and Anti-Inflammatory Effects of Exercise in Diabetic Patients. Exp Diabetes Res. 2012;2012:1–16. https://doi.org/10.1155/2012/941868 . [In persian]

دوره 15، شماره 4
دی 1402
صفحه 85-100