نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه صنعتی شاهرود، سمنان، ایران.

2 عضو هیئت علمی دانشگاه صنعتی شاهرود

3 استادیار فیزیولوژی ورزشی دانشگاه صنعتی شاهرو د

چکیده

زمینه: تنظیم مثبت نشانگرهای مرتبط با پیری در بافت چربی، اختلال در بافت چربی را به همراه دارد. به نظر می‌رسد فعالیت ورزشی، در بیان ژن‌های مرتبط با پیری اثرگذار است.

هدف: هدف از پژوهش حاضر مقایسه تأثیر تمرین استقامتی و مقاومتی بر بیان ژن‌های مرتبط با پیری در بافت چربی احشایی موش‌های صحرایی چاق می‌باشد.

روش پژوهش: در این پژوهش تجربی، 24 سر رت نر چاق، به سه گروه تمرین استقامتی، تمرین مقاومتی و کنترل تقسیم شدند. گروه‌های تمرینی به مدت هشت هفته و پنج روز در هفته به فعالیت پرداختند. تمرین گروه استقامتی، شامل دویدن بر تردمیل به صورت مداوم و تمرین گروه مقاومتی شامل، بالا رفتن از نردبان مقاومتی بود. در همین زمان، گروه کنترل هیچگونه تمرینی نداشت. میزان بیان ژن‌های P53 ، P16وIL-6 در بافت احشایی موش‌های صحرایی چاق اندازه‌گیری شد و داده‌های به دست آمده با استفاده از آزمون آماری آنالیز واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنی‌داری ۰۵/۰ ارزیابی شدند.

یافته ها: یافته‌‌های پژوهش حاضر نشان داد، مقادیر بیان ژن‌های P53 ، P16وIL-6 ، در گروه‌های تمرین استقامتی (001/0= p،006/0=p، 001/0=p) و تمرین مقاومتی (001/0= p،038/0=p، 001/0=p) نسبت به گروه کنترل کاهش معنی‌داری داشت. همچنین تفاوت معنی‌داری در میزان بیان ژن‌ها، بین گروه‌های تمرینی مشاهده نشد (439/0= p، 584/0= p،300/0=p).

نتیجه گیری: با توجه به نتایج حاصل از مطالعه حاضر به نظر می‌رسد، تمرین استقامتی و مقاومتی در کاهش بیان ژن‌های مرتبط با پیری نقش دارند؛ که این اثرگذاری در تمرینات استقامتی و مقاومتی تفاوتی نداشت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Comparing Endurance and Resistance training on the Expression of Senescence-Related Genes in the Visceral Adipose Tissue of obese rats

نویسندگان [English]

  • Nastaran Ghorbani Dasht Bayaz 1
  • adel donyaie 2
  • Elham Vosadi 3

1 Faculty of Physical Education and sport sciences, Shahrood University of Technology, Semnan, Iran.

2 assistant professor of Shahrood University of Technology

3 Assistant Professor of Sport Physiology Shahro University of Technology

چکیده [English]

Background: Positive regulation of senescence-related markers leads to adipose tissue disorders. Exercise seems to improve aging in adipose tissue.

Aims: The aim of this study was to compare the effect of endurance and resistance training on the expression of senescence-related genes.

Methods: In this experimental study, Twenty-four obese rats were divided into three groups: (1) Endurance Training, (2) Resistance Training, and (3) Control groups. Training groups trained for 8 weeks and 5 days a week. The Endurance group exercise program consists of Running on a treadmill and the Resistance group exercise program is included Climbing a resistance ladder. While, the control group had no training. The expression of P53, P16, and IL-6 genes in visceral tissue of obese rats were measured by Real-time PCR analysis. Finally, the data were analyzed by one-way ANOVA and Tukey post hoc test and statistical differences were considered significant at P<0.05.

Results: The results of this study showed the expression levels of P53, P16, and IL-6 genes in the Endurance Training group (P=0.001, P=0.006, P=0.001) and Resistance Training group (P=0.001, P=0.038, P=0.001) had a significant decrease compared to the control group. There was also no significant difference in the expression of P53, P16, and IL-6 genes between the endurance and resistance groups (P=0.439, P=0.584, P=0.300).

Conclusion: According to the results of the present study, it seems that endurance and resistance training plays a role in reducing the expression of senescence-related genes, which This effect was no different in endurance and resistance training.

کلیدواژه‌ها [English]

  • Senescence-Related Genes
  • Endurance Training
  • Resistance Training
  • Obese rats

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