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اثر همزمان تمرین هوازی و مصرف عصارۀ سبوس برنج بر بیان ژن‌های التهابی بافت چربی سفید در رت‌های مادۀ چاق

نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I

نویسندگان

1 گروه تربیت بدنی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

2 نویسنده مسئول، گروه تربیت بدنی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران.

3 گروه فیزیولوژی ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مقدمه: چاقی و التهاب یک ریسک‌فاکتور مهم بیماری‌هاست. تمرینات هوازی (AE) و گیاهان دارویی می‌توانند التهاب ناشی از چاقی را کاهش دهند. هدف از این تحقیق بررسی تأثیر AE و سبوس برنج (RBE) بر بیان ژن‌های التهابی در بافت چربی سفید موش‌های صحرایی مادۀ چاق بود.
روش پژوهش: 30 موش صحرایی مادۀ ویستار به‌طور تصادفی به پنج گروه کنترل تغذیه‌شده با رژیم غذایی طبیعی، کنترل رژیم غذایی پرچرب (HFD)، HFD+(AE)، HFD+(RBE) و HFD (AE)+(RBE) تقسیم شدند. موش‌ها به مدت چهار هفته روی تردمیل دویدند و (RBE) دریافت کردند. به‌منظور القای چاقی از روغن سرخ‌کردنی پالم استفاده شد (0.5 ml/g). پس از قربانی‌سازی، بافت چربی سفید برای اندازه‌گیری بیان ژن‌های IL6، TNF-α و TLR4 استخراج شد.
 یافته‌ها: HFD سبب افزایش معنا‌داری در بیان ژن IL-6، TNF-α و TLR4 شد (P=0.001). تم)رین هوازی بیان ژن‌های IL-6(P=0.001)، TNF-a(P=0.041) و TLR-4(P=0.032) را به‌طور معنا‌داری کاهش داد. عصارۀ سبوس برنج بیان ژن‌های IL-6(P=0.001)،TNF-α  (P=0.003) وTLR-4  (P=0.011) را به‌طور معناداری کاهش داد. بیان ژن IL-6(P=0.002)، TNF-α (P=0.005) وTLR4 (P=0.011) در گروهAE + RBE نسبت به گروه کنترل HFD به‌طور معنا‌داری کمتر بود. تعامل AE و RBE بر بیان این ژن‌ها معنادار نبود.
نتیجه‌گیری: (AE) و (RBE) بیان ژن واسطه‌های التهابی ناشی از HFD را در بافت چربی سفید کاهش می‌دهند. استفاده از مصرف AE و RBE به‌عنوان یک راه‌حل پیشگیرانه و درمانی در شرایط چاقی توصیه می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Simultaneous Effect of Aerobic Exercise and Rice Bran Extract Consumption on Inflammatory Genes Expression in White Adipose Tissue of Obese Female Rats

نویسندگان [English]

  • Mojtaba Moazamigoodarzi 1
  • shahram gholamrezaei darsara 2
  • Mohammad Ali ََAzarbayjani 3
  • Alireza Elmiye 1

1 Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran

3 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

چکیده [English]

Introduction: Obesity and inflammation is an important risk factor for diseases. Aerobic exercise (AE) and medicinal herbs can reduce obesity-induced inflammation. This study aimed to investigate the effect of AE and Rice Bran Extract (RBE) on the inflammatory gene expression in white adipose tissue of obese female rats.
Methods: Thirty female Wistar rats were randomly divided into five groups including Control with a natural diet, Control with a High-Fat Diet (HFD), HFD + AE, HFD + RBE, and HFD + AE + RBE. Rats ran on a treadmill for four weeks and received RBE. To induce obesity, frying palm oil was used (0.5 ml/g). After euthanasia, the white adipose tissue was extracted to measure the IL6, TNF-α, and TLR4 gene expression.
 Results: HFD caused a significant increase in the IL-6, TNF-α, and TLR4 gene expression (P=0.001). AE significantly decreased IL-6 (P=0.001), TNF-α (P=0.041), and TLR4 (P=0.032) gene expression. RBE significantly decreased the IL-6 (P=0.001), TNF-α (P=0.003), and TLR-4 (P=0.011) gene expression. IL-6 (P=0.002), TNF-α (P=0.005), and TLR4 (P=0.011) gene expression was significantly decreased in the AE + RBE group compared to the control group with HFD, However, AE and RBE interaction in the expression of these genes was not statistically significant.
Conclusion: AE and RBE decrease the gene expression of inflammatory mediators caused by HFD in white adipose tissue. The AE and RBE consumption is recommended as a preventive and therapeutic solution in obesity conditions.

کلیدواژه‌ها [English]

  • aerobic exercise
  • rice bran
  • IL-6
  • TNF-a and TLR-4
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نشریه علوم زیستی ورزشی
دوره 15، شماره 4 - شماره پیاپی 98
دی 1402
صفحه 5-20
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  • تاریخ دریافت: 14 اسفند 1401
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