نشریه علوم زیستی ورزشی

اثر همزمان تمرین هوازی و مصرف عصارۀ سبوس برنج بر بیان ژن‌های التهابی بافت چربی سفید در رت‌های مادۀ چاق

نوع مقاله : مقاله پژوهشی

نویسندگان

مجتبی معظمی گودرزی 1
شهرام غلامرضایی دارسرا 2
محمدعلی آذربایجانی 3
علیرضا علمیه 1

1 گروه تربیت بدنی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

2 نویسنده مسئول، گروه تربیت بدنی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران.

3 گروه فیزیولوژی ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران

https://doi.org/10.22059/jsb.2023.354829.1578
چکیده
مقدمه: چاقی و التهاب یک ریسک‌فاکتور مهم بیماری‌هاست. تمرینات هوازی (AE) و گیاهان دارویی می‌توانند التهاب ناشی از چاقی را کاهش دهند. هدف از این تحقیق بررسی تأثیر AE و سبوس برنج (RBE) بر بیان ژن‌های التهابی در بافت چربی سفید موش‌های صحرایی مادۀ چاق بود.
روش پژوهش: 30 موش صحرایی مادۀ ویستار به‌طور تصادفی به پنج گروه کنترل تغذیه‌شده با رژیم غذایی طبیعی، کنترل رژیم غذایی پرچرب (HFD)، HFD+(AE)، HFD+(RBE) و HFD (AE)+(RBE) تقسیم شدند. موش‌ها به مدت چهار هفته روی تردمیل دویدند و (RBE) دریافت کردند. به‌منظور القای چاقی از روغن سرخ‌کردنی پالم استفاده شد (0.5 ml/g). پس از قربانی‌سازی، بافت چربی سفید برای اندازه‌گیری بیان ژن‌های IL6، TNF-α و TLR4 استخراج شد.
 یافته‌ها: HFD سبب افزایش معنا‌داری در بیان ژن IL-6، TNF-α و TLR4 شد (P=0.001). تم)رین هوازی بیان ژن‌های IL-6(P=0.001)، TNF-a(P=0.041) و TLR-4(P=0.032) را به‌طور معنا‌داری کاهش داد. عصارۀ سبوس برنج بیان ژن‌های IL-6(P=0.001)،TNF-α  (P=0.003) وTLR-4  (P=0.011) را به‌طور معناداری کاهش داد. بیان ژن IL-6(P=0.002)، TNF-α (P=0.005) وTLR4 (P=0.011) در گروهAE + RBE نسبت به گروه کنترل HFD به‌طور معنا‌داری کمتر بود. تعامل AE و RBE بر بیان این ژن‌ها معنادار نبود.
نتیجه‌گیری: (AE) و (RBE) بیان ژن واسطه‌های التهابی ناشی از HFD را در بافت چربی سفید کاهش می‌دهند. استفاده از مصرف AE و RBE به‌عنوان یک راه‌حل پیشگیرانه و درمانی در شرایط چاقی توصیه می‌شود.

کلیدواژه‌ها

تمرین هوازی
سبوس برنج
6 IL-
TNF-a و TLR-4
موضوعات

عنوان مقاله English

The Simultaneous Effect of Aerobic Exercise and Rice Bran Extract Consumption on Inflammatory Genes Expression in White Adipose Tissue of Obese Female Rats

نویسندگان English

Mojtaba Moazamigoodarzi 1
shahram gholamrezaei darsara 2
Mohammad Ali ََAzarbayjani 3
Alireza Elmiye 1
1 Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
چکیده English

Introduction: Obesity and inflammation is an important risk factor for diseases. Aerobic exercise (AE) and medicinal herbs can reduce obesity-induced inflammation. This study aimed to investigate the effect of AE and Rice Bran Extract (RBE) on the inflammatory gene expression in white adipose tissue of obese female rats.
Methods: Thirty female Wistar rats were randomly divided into five groups including Control with a natural diet, Control with a High-Fat Diet (HFD), HFD + AE, HFD + RBE, and HFD + AE + RBE. Rats ran on a treadmill for four weeks and received RBE. To induce obesity, frying palm oil was used (0.5 ml/g). After euthanasia, the white adipose tissue was extracted to measure the IL6, TNF-α, and TLR4 gene expression.
 Results: HFD caused a significant increase in the IL-6, TNF-α, and TLR4 gene expression (P=0.001). AE significantly decreased IL-6 (P=0.001), TNF-α (P=0.041), and TLR4 (P=0.032) gene expression. RBE significantly decreased the IL-6 (P=0.001), TNF-α (P=0.003), and TLR-4 (P=0.011) gene expression. IL-6 (P=0.002), TNF-α (P=0.005), and TLR4 (P=0.011) gene expression was significantly decreased in the AE + RBE group compared to the control group with HFD, However, AE and RBE interaction in the expression of these genes was not statistically significant.
Conclusion: AE and RBE decrease the gene expression of inflammatory mediators caused by HFD in white adipose tissue. The AE and RBE consumption is recommended as a preventive and therapeutic solution in obesity conditions.

کلیدواژه‌ها English

aerobic exercise
rice bran
IL-6
TNF-a and TLR-4

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