نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 نویسندۀ مسؤول، گروه فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه تبریز، تبریز، ایران.
2 گروه فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه تبریز، تبریز، ایران.
چکیده
مقدمه: هدف از پژوهش حاضر تعیین اثر تمرین ترکیبی بر بیان ژن برخی از سایتوکاین‌های پیش‌التهابی و ارزیابی سطوح هورمونی در زنان یائسۀ چاق است.
روش پژوهش: در این تحقیق نیمه‌تجربی 30 زن یائسۀ کم‌تحرک و چاق انتخاب شده و به‌طور تصادفی به دو گروه تجربی (15 نفر) و گروه کنترل (15 نفر) تقسیم شدند. گروه تجربی به مدت 12 هفته، پنج روز در هفته، شامل دو جلسه تمرین مقاومتی به مدت 60 دقیقه و 3 جلسه تمرین هوازی به مدت 30 دقیقه، شرکت کردند. پیش و پس از مداخله، نمونۀ خون برای ارزیابی استرادیول، استرادیول آزاد و میزان بیان mRNA ژن‌های اینترلوکین-6 (IL-6) و تومور نکروزدهندۀ آلفا (TNF-α) از داوطلبان اخذ شد. بیان کمی ژن با استفاده از روش 2-∆∆ct و از طریق نرم‌افزار REST ارزیابی شد. از آزمون شاپیروویلک برای بررسی توزیع طبیعی داده‌ها و از تحلیل واریانس در اندازه‌گیری‌های مکرر برای مقایسة گروه‌ها در سطح معناداری α<0.05 استفاده شد.
یافته‌ها: در پاسخ به پروتکل تمرینی، کاهش در تودۀ چربی (6/9- درصد) و افزایش تودۀ بدون چربی (2/4+ درصد) در گروه تجربی مشاهده شد. سطوح استرادیول آزاد (10/70- درصد ) و میزان بیان ژن IL-6 ( P=0.003) و TNF-α (P=0.000) کاهش معناداری در گروه تجربی نسبت به گروه کنترل داشت. اما تفاوت معناداری در استرادیول برای گروه تجربی وجود نداشت (P<0.05).
نتیجه‌گیری: به‌نظر می‌رسد تمرین ترکیبی و کاهش تودۀ چربی ناشی از فعالیت، پروفایل هورمون‌های جنسی و میزان بیان ژن IL-6 و TNF-α را در زنان یائسۀ مستعد BC تغییر می‌دهد. بنابراین این تحقیق شواهد بیشتری از تعامل پیچیدۀ بین نشانگرهای التهابی، بافت چربی و تودۀ عضلانی را در این گروه از زنان ارائه می‌کند.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The Effect of Twelve-Week Concurrent Training on the Regulation of Inflammatory Markers in Postmenopausal Women Prone to Breast Cancer
نویسندگان [English]
- Vahid Sari-Sarraf 1
- javad vakili 2
- Maryam Heidaryan 2
1 Corresponding Author, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.
2 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.
چکیده [English]
Introduction: The present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.
Methods: In this quasi-experimental research, 30 sedentary and obese PW were recruited and randomly divided into Concurrent Training (CT, n=15) and Control (CG, n=15) groups. Participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. Blood samples were taken from the volunteers to measure estradiol, free estradiol, and mRNA expression levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Quantitative gene expression was evaluated using the 2-∆∆ct method and REST software. The Shapiro-Wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (ANOVA) tests were used to compare the groups at a significance level of α=0.05.
Results: In response to the training protocol, the CT group experienced a reduction in fat mass (-9.6%; P<0.05) and an increase in lean body mass (2.4%; P<0.05). The Circulating levels of free Estradiol (−10.70%; P<0.05), and levels of gene expression of IL-6 (P=0.03) and TNF-α (P=0.000) significantly decreased in the CT group compared with the CG group. But there was no significant difference in Estradiol in the CT group compared with the CG group (P<0.05).
Conclusion: The concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of IL-6 and TNF-α in PW Prone to breast cancer. Thus, this study provides additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle mass in PW Prone to breast cancer.
Introduction: The present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.
Methods: In this quasi-experimental research, 30 sedentary and obese PW were recruited and randomly divided into Concurrent Training (CT, n=15) and Control (CG, n=15) groups. Participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. Blood samples were taken from the volunteers to measure estradiol, free estradiol, and mRNA expression levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Quantitative gene expression was evaluated using the 2-∆∆ct method and REST software. The Shapiro-Wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (ANOVA) tests were used to compare the groups at a significance level of α=0.05.
Results: In response to the training protocol, the CT group experienced a reduction in fat mass (-9.6%; P<0.05) and an increase in lean body mass (2.4%; P<0.05). The Circulating levels of free Estradiol (−10.70%; P<0.05), and levels of gene expression of IL-6 (P=0.03) and TNF-α (P=0.000) significantly decreased in the CT group compared with the CG group. But there was no significant difference in Estradiol in the CT group compared with the CG group (P<0.05).
Conclusion: The concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of IL-6 and TNF-α in PW Prone to breast cancer. Thus, this study provides additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle mass in PW Prone to breast cancer.
Introduction: The present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.
Methods: In this quasi-experimental research, 30 sedentary and obese PW were recruited and randomly divided into Concurrent Training (CT, n=15) and Control (CG, n=15) groups. Participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. Blood samples were taken from the volunteers to measure estradiol, free estradiol, and mRNA expression levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Quantitative gene expression was evaluated using the 2-∆∆ct method and REST software. The Shapiro-Wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (ANOVA) tests were used to compare the groups at a significance level of α=0.05.
Results: In response to the training protocol, the CT group experienced a reduction in fat mass (-9.6%; P<0.05) and an increase in lean body mass (2.4%; P<0.05). The Circulating levels of free Estradiol (−10.70%; P<0.05), and levels of gene expression of IL-6 (P=0.03) and TNF-α (P=0.000) significantly decreased in the CT group compared with the CG group. But there was no significant difference in Estradiol in the CT group compared with the CG group (P<0.05).
Conclusion: The concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of IL-6 and TNF-α in PW Prone to breast cancer. Thus, this study provides additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle mass in PW Prone to breast cancer.
کلیدواژه‌ها [English]
- breast cancer risk
- concurrent training
- Estradiol
- pro-inflammatory cytokines
- menopause
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