نشریه علوم زیستی ورزشی

تاثیر هشت هفته تمرینات استقامتی، مقاومتی و تناوبی شدید بر بیان ژن SREBP-1 و 12.13.diHome رت‌های نرویستارچاق

نوع مقاله : مقاله پژوهشی

نویسندگان

محسن اکبری 1
امیر رشید لمیر 2
ناهید بیژه 1
سیدعلیرضا حسینی کاخک 3

1 گروه فیزیولوژی ورزشی، دانشکدۀ علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران.

2 نویسنده مسؤول، گروه فیزیولوژی ورزشی، دانشکدۀ علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران.

3 گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه حکیم سبزواری، سبزوار، ایران

https://doi.org/10.22059/jsb.2023.354848.1572
چکیده
مقدمه: چاقی و اضافه وزن یک اختلال متابولیکی است که سطوح بافت چربی در این اختلال تاثیرگذار است. هدف از پژوهش حاضر، تاثیر هشت هفته تمرینات استقامتی، مقاومتی و تناوبی شدید بر بیان ژن SREBP-1 و 12.13.diHome رت‌های نرویستارچاق بود.

روش‌شناسی ؛ پژوهش حاضر، 36 موش صحرایی نژاد ویستارنر چاق با سن هشت هفته و وزن 40±325 گرم، به صورت تصادفی به چهار گروه؛ استقامتی (10سر)، مقاومتی (8سر)، تناوبی شدید (10سر) و کنترل (8سر) تقسیم شدند. موش-های گروه‌های تجربی به مدت هشت هفته، هفته‌ای پنج جلسه تمرینات استقامتی با شدت70 تا80درصد سرعت بیشینه، مقاومتی با شدت 50 تا 120 درصدوزن بدن و تناوبی شدید با شدت85 تا 90 درصد حداکثر سرعت را انجام دادندو برای اندازه‌گیری بیان ژن SREBP-1 و 12.13.diHome ازروش Real Time-PCR استفاده شد. از روش آماری تحلیل واریانس یک‌طرفه و آزمون تعقیبی توکی جهت تعیین اختلاف بین‌گروها در سطح معنی‌داری05/0≤P استفاده شد.

یافته‌ها؛ نتایج پژوهش حاضرنشان داد هر سه مدل تمرینی استقامتی، مقاومتی و تناوبی شدید سبب افزایش معنادار بیان ژن 12.13.diHome و کاهش معنادار SREBP-1 نسبت به گروه کنترل شد اما تفاوتی بین گروه‌های تجربی مشاهده نشد

بنابرنتایج این پژوهش، پیشنهاد می‌شود از تمرینات استقامتی، مقاومتی و تناوبی شدید با کاهش بیان ژن SREBP-1 و افزایش 12.13.diHome که فاکتورهای تاثیرگذار بر لیپولیز می‌باشند و در افزایش لیپولیز تاثیردارند، در جهت کاهش وزن استفاده شود.

کلیدواژه‌ها

استقامتی
مقاومتی
HIIT
12.13.diHome
SREBP-1
چاقی
موضوعات

عنوان مقاله English

The Effect of Eight-Week Endurance, Resistance and High-Intensity Interval Training on SREBP-1 and 12.13-diHome Gene Expression in Male Obese Vistar Rats

نویسندگان English

Mohsen Akbari 1
Amir Rashid Lamir 2
nahid bijeh 1
Alireza Hosseini kakhk 3
1 Department of Sports Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Corresponding Author, Department of Sports Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
3 Department of Exercise Physiology, Faculty of Sport Science, Hakim Sabzevari University, Sabzevar, Iran
چکیده English

Introduction: Obesity and overweight are metabolic disorders in which fat tissue levels are influential in this disorder. The current research aimed to investigate the effect of eight weeks of endurance, resistance, and high-intensity interval training on the SREBP-1 and 12.13.diHome gene expression in male obese Wistar rats.
Methods: In the current study, 36 obese male Wistar rats (eight weeks old rats with a weight of 325 ± 40 grams) were randomly divided into four Endurance (n=10), Resistance (n=8), High-Intensity Interval Training (n=10) and Control (n=8) groups. The rats in the experimental groups performed five sessions of endurance training at an intensity of 70-80% of the maximum speed, resistance training at an intensity of 50-120% of the body weight, and HIIT at an intensity of 85-90% of the maximum speed for eight weeks. To measure SREBP-1 and 12.13.diHome gene expression, the Real Time-PCR method was used. The statistical method of one-way analysis of variance with Tukey's post hoc test was used to determine the difference between groups at a significant level of α= 0.05.
Results: The results of the present study showed that all three training models of endurance, resistance, and HIIT caused a significant increase in the expression of the 12.13.diHome gene and a significant decrease in SREBP-1 compared with the control group (P<0.05), but no significant difference was observed among the experimental groups (P>0.05).
Conclusion: To lose weight, it is suggested to use endurance, resistance, and HIIT training to reduce the expression of the SREBP-1 gene and increase 12.13.diHome, which are factors influencing lipolysis and lipogenesis.

کلیدواژه‌ها English

12.13-diHOME
Endurance
HIIT
Resistance
SREBP-1
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