نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 گروه فیزیولوژی ورزش، دانشگاه پیام نور، تهران، ایران.
2 نویسندۀ مسؤول، گروه فیزیولوژی ورزش، دانشگاه پیام نور، تهران، ایران.
چکیده
مقدمه: عفونت در دوران نوزادی اضطراب را افزایش می‌دهد. هدف از پژوهش حاضر، بررسی تأثیر یک دوره تمرینات شنا و داروی مینوسایکلین پس از عفونت دوران کودکی بر سطح اضطراب و سطح مالون دی‌آلدئید در هیپوکامپ و پرفرونتال کورتکس موش‌های نژاد NMRI بود.
روش پژوهش: به‌منظور القای عفونت، در روزهای سوم و پنجم پس از تولد حیوانات به مدت 15 روز لیپوپلی ساکارید دریافت کردند. حیوانات به پنج گروه کنترل، لیپوپلی ساکارید، لیپوپلی ساکارید+مینوسیکلین، لیپوپلیساکارید+ورزش و لیپوپلی ساکارید+ورزش+ مینوسایکلین تقسیم شدند. طول دوره چهار هفته و دوز مینوسایکلین 20 میلی‌گرم بر کیلوگرم بود. از تست اضطراب فضای باز به‌منظور اندازه‌گیری سطوح اضطراب استفاده شد. آنالیز واریانس یکطرفه با آزمون تعقیبی توکی در سطح معنا‌داری 0.05 استفاده شد.
یافته‌ها: سطوح MDA در گروه لیپوپلیساکارید در هر دو بخش هیپوکامپ و پری‌فرونتال بالاترین مقدار را در مقایسه با گروه کنترل داشت (0.05 ≥P). درصد تغییرات MDA هیپوکامپ گروه لیپوپلیساکارید + ورزش + مینوسایکلین نسبت به گروه کنترل 14 درصد کاهش و MDA پری فرونتال گروه لیپوپلیساکارید + ورزش + مینوسایکلین نسبت به گروه کنترل 20 درصد افزایش داشت. زمان سپری‌شده و تعداد ورود در تست اضطراب فضای باز در گروه لیپوپلیساکارید + ورزش + مینوسایکلین در مقایسه با سایر گروه‌ها افزایشی و در گروه لیپوپلیساکارید کاهشی بود (0.05 ≥P).
نتیجه‌گیری: نتایج نشان داد ورزش شنا و داروی مینوسایکلین می‌تواند عوارض ناشی از اضطراب ناشی از عفونت دوران کودکی را با تغییرات MDA تغییر دهد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The Effect of a Course of Swimming and Minocycline Exercises After Childhood Infection on Anxiety and Malondialdehyde Levels in the Hippocampus and Peripheral Cortex of NMRI Mice
نویسندگان [English]
- Rogayyeh Mahdavi 1
- Saeid Naghibi 2
- Ali Barzegari 1
1 Department of Exercise Physiology, Payame Noor University, Tehran, Iran.
2 Corresponding Author, Department of Exercise Physiology, Payame Noor University, Tehran, Iran.
چکیده [English]
Introduction: Infection in infancy increases the level of anxiety. The aim of this study was to evaluate the effect of a course of swimming exercises and minocycline medication after childhood infection on the level of anxiety and malondialdehyde levels in the hippocampus and peripheral cortex of NMRI mice.
Methods: In order to induce infection, on the third and fifth day after birth, the animals received lipopolysaccharide for 15 days. Animals were divided into 5 groups, consist of control, LPS; LPS+ Min; LPS + Ex and LPS+ Ex +Min were divided. The duration of the course was 4 weeks and the dose of minocycline was 20 mg/kg. The Open Field test anxiety was used to measure anxiety levels. One-way ANOVA with Tukey's post hoc test at a significance level of 0.05 was used.
Results: MDA levels in the LPS group were the highest in both the hippocampus and prefrontal sections compared to the Control group (P ≥ 0.05). The percentage of hippocampal MDA changes in the LPS+Min+EX group decreased by 14% compared to the control group, and the prefrontal MDA of the LPS+Min+EX group increased by 20% compared to the control group. So that the elapsed time and the number of entries in the Open Field test were increased in the LPS+Min+Ex group compared to other groups and decreased in the LPS group (P ≤ 0.05).
Conclusion: The results showed that swimming exercise and minocycline can change the effects of anxiety caused by childhood infection with changes in MDA.
کلیدواژه‌ها [English]
- Anxiety
- Childhood Infection
- Malondialdehyde
- Minocycline
- Swimming Exercises
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