نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 دانشیار گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه ولی عصر (عج)، رفسنجان، ایران

2 دانشجوی کارشناسی ارشد، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، کرمان، ایران. مرکزتحقیقات فیزیولوژی، پژوهشکده نوروفارماکولوژی، دانشگاه علوم پزشکی کرمان، کرمان، ایران

چکیده

اختلال در عملکرد عصبی-عضلانی به‌همراه کاهش فعالیت بدنی سبب بروز پیامدهای چشمگیری بر عملکرد عضلانی می‌شود. ازاین‌رو هدف از پژوهش حاضر بررسی اثر یک دوره تمرین مقاومتی و ترکیبی بر بیان ژن‌های MST1 و MAFbx عضلة پلانتاریس و آزمون‌های رفتاری موش‌های صحرایی نر ویستار پیش از کاهش فعالیت بدنی به شکل لیگاسیون عصب نخاعی بود. 24 سر موش صحرایی نر 8 هفته‌ای با وزن 20±250 گرم به روش تصادفی ساده به 3 گروه مساوی تقسیم شدند: 1. گروه شم – لیگاسیون (Sham-SNL)، 2. گروه تمرین مقاومتی - لیگاسیون (SNL-S) و 3. گروه تمرین ترکیبی - لیگاسیون (SNL-CO). در هر سه گروه، پس از اتمام پروتکل تمرین، سه شاخه عصب سیاتیک به‌طور محکم گره زده شد. مدت پروتکل SNL، 4 هفته بود. در پایان پروتکل، عضلة پلانتاریس استخراج شد. به‌منظور اندازه‌گیری بیان ژن‌های موردنظر از روش Real-Time PCR استفاده شد. بیان ژن MST1 و MAFbx در گروه Sham-SNL نسبت به گروه‌های تمرین به‌طور غیرمعنا‌داری بالاتر بود (05/0<P). از طرف دیگر، نتایج آزمون آنوای یکطرفه و آزمون توکی نشان داد که اختلاف معنا‌داری در هایپرآلژزیای حرارتی و آلوداینیای مکانیکی بین گروه شم و گروه‌های تمرین وجود دارد (05/0>P). با توجه به تغییر معنا‌دار آزمون‌های رفتاری در گروه شم نسبت به گروه‌های تمرین می‌توان ادعا کرد که تمرینات مقاومتی و ترکیبی برای بهبود درد نوروپاتیک می‌تواند مفید باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of two training models on the MST1 and MAFbx genes expression in the Plantaris muscle and behavioral tests of male Wistar rats before decreased physical activity in the form of Spinal Nerve Ligation

نویسندگان [English]

  • Abdolreza Kazemi 1
  • Asma Moghadam 2

1 Associate Prof, Dept of Physical Education, Faculty of Letters and Humanities, Vali E Asr University, Rafsanjan, Iran.

2 MSc, Department of Exercise Physiology, Kerman Branch, Islamic Azad University, Kerman,I ran, Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

چکیده [English]

Neuromuscular dysfunction along with reduced physical activity has significant consequences on muscle function. Therefore, the aim of the present study was to investigate the effect of resistance and combined training on the MST1 and MAFbx genes expression in Plantaris muscle and behavioral tests in male Wistar rats before decreased physical activity in the form of spinal cord ligation (SNL).twenty four 8-week-old male rats with the mean weight of 250± 20 grams were randomly devided into 3 groups: 1) Sham-Spinal Nerve Ligation (Sham-SNL), 2) Resistance Training-Spinal Nerve Ligation (SNL-S), 3) Combined training-Spinal Nerve Ligation (SNL-CO). In all three groups, three branches of the sciatic nerve of rats were tightly tied after the end of training protocol. The duration of the SNL protocol was 4 weeks. At the end of the protocol, Plantaris muscle was extracted. Real-Time PCR method was used to measure the mRNA expression of the genes. MST1 and MAFbx gene expression in the Sham-SNL group was non-significantly higher than the training groups (P <0.05). On the other hand, the results of one-way ANOVA and Tukey’s tests showed that there is a significant difference in thermal hyperalgesia and mechanical allodynia test between Sham-SNL group and training groups (P <0.05). Considering the significant change of behavioral tests in the sham group compared to the training groups, it can be claimed that resistance and combination training can be useful for improving neuropathic pain.

کلیدواژه‌ها [English]

  • Behavioral tests
  • combined and resistance training
  • SNL
  • MST1
  • MAFbx
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