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تأثیر تمرینات ورزشی با شدت کم ، متوسط و زیاد بر شاخص‌های آپوپتوز بافت بیضۀ موش‌های صحرایی نر

نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I

نویسندگان

1 گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشکدۀ علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران

2 نویسنده مسئول. گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشکدۀ علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران

3 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

چکیده

مقدمه: تأثیر تمرینات ورزشی با شدت‌های مختلف بر القا و یا مهار فرایند آپوپتوز وابسته به میتوکندری در بافت بیضه تاکنون ناشناخته مانده است. هدف از پژوهش حاضر، بررسی تأثیر تمرین تداومی با شدت کم (LICT)، متوسط(MICT) و زیاد (HICT) بر فرایند آپوپتوز در بافت بیضۀ موش‌های صحرایی بود.
روش پژوهش: بدین‌منظور، 24 سر موش صحرایی نر نژاد ویستار به‌صورت تصادفی به چهار گروه LICT، MICT، HICT و کنترل تقسیم شدند. گروه LICT با شدت 50-60 درصد حداکثر سرعت ، گروه MICT با شدت 65-70 درصد حداکثر سرعت  و گروه HICT با شدت 80-85 درصد حداکثر سرعت  تمرین کردند. بیان پروتئین و ژنBax ، Bcl-2، کاسپاز ـ3 با روش وسترن بلات و PCR کمی اندازه‌گیری شد. داده‌های پژوهش با آزمون آماری ANOVA و آزمون تعقیبی توکی تجزیه‌وتحلیل شد.
یافته‌ها : نتایج نشان داد که در گروه LICT و MICT بیان Bcl2، Bax و کاسپاز ـ3 تغییر معناداری در مقایسه با گروه کنترل نداشت (05/0P>). در گروه HICT بیان Bcl-2 کاهش معنادار (01/0P=) و بیان Bax و کاسپاز-3 افزایش معناداری (05/0P=) داشت. همچنین تعداد سلول‌های آپوپتوتیک در LICT و MICT تغییر معناداری را نشان نداد، ولی در گروه HICT افزایش معناداری داشت (001/0P=).
نتیجه‌گیری: به‌نظر می‌رسد هشت هفته تمرین LICT و MICT هیچ تأثیر معناداری در بروز یا القای فرایند آپوپتوز وابسته به میتوکندری در بافت بیضه ندارد. درحالی‌که گروه HICT با کاهش بیان Bcl2 و افزایش بیان Bax و کاسپازـ3، فرایند آپوپتوز را القا می‌کند و تعداد سلول‌های آپوپتوتیک را در بافت بیضه افزایش می‌دهد. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Low, Moderate and High Intensity Exercise Trainings on Apoptotic Indices of Testicular Tissue in Male Rats

نویسندگان [English]

  • Yousef Saberi 1
  • Javad Toloueiazar 2
  • Mazdak Razi 3
  • Asghar Tofighi 1

1 Department of Exercise Physiology and Corrective Exercises, Faculty of Sports Sciences, Urmia University, Urmia, Iran.

2 Corresponding Author. Department of Exercise Physiology and Corrective Exercises, Faculty of Sports Sciences, Urmia University, Urmia, Iran.

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

چکیده [English]

Introduction: The effect of sports training with different intensities on the induction or inhibition of mitochondria-dependent apoptosis process in testicular tissue has remained unknown.This study aimed to investigate the effect of continuous training with low intensity (LICT), moderate (MICT), and high intensity (HICT) on the apoptosis process in the testicular tissue of rats.
Methods: For this purpose, 24 male Wistar rats were randomly divided into LICT, MICT, HICT, and Control groups. The LICT, MICT, and HICT groups were trained with an intensity of 50-60%, 65-70%, and 80-85% of the maximum velocity, respectively. Protein and gene expression of Bax, Bcl-2, and Caspase-3 were measured by Western Blot and quantitative PCR methods. The research data were analyzed by ANOVA and Tukey's post hoc test. 
Results: The LICT and MICT groups showed no significant statistical changes in the Bcl-2, Bax, and Caspase-3 expression levels compared to the control group (P>0.05). However, In the HICT group, the expression of Bcl-2 decreased significantly (P=0.01) and the expression of Bax and Caspase-3 increased significantly (P<0.05). Also, the number of apoptotic cells in LICT and MICT did not show a significant change, but it increased significantly in the HICT group (P=0.001). 
Conclusion: It seems that eight weeks of LICT and MICT has no significant effect on the occurrence or induction of mitochondria-dependent apoptosis process in testicular tissue. While the HICT group induces the apoptosis process by decreasing the expression of Bcl-2 and increasing the expression of Bax and Caspase-3 and increasing the number of apoptotic cells in the testicular tissue.

کلیدواژه‌ها [English]

  • Exercise Trainings
  • Testicular tissue
  • Intrinsic Apoptosis Pathway
  • Wistar rats
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نشریه علوم زیستی ورزشی
دوره 14، شماره 2
شهریور 1401
صفحه 43-60
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سابقه مقاله
  • تاریخ دریافت: 12 بهمن 1400
  • تاریخ بازنگری: 14 اسفند 1400
  • تاریخ پذیرش: 02 اردیبهشت 1401
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