نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 گروه فیزیولوژی ورزشی، دانشکدۀ علوم ورزشی و تندرستی، دانشگاه تهران، تهران، ایران.
2 نویسندۀ مسؤول، گروه فیزیولوژی ورزشی، علوم ورزشی و تندرستی، دانشگاه تهران، تهران، ایران.
چکیده
مقدمه: استرس اکسیداتیو و تولید گونه‌های اکسیژن (ROS) و نیتروژن (RNS) واکنش‌پذیر، نقش مهمی در پاتوژنز و پیشرفت بیماری ام‌اس دارد. هدف از پژوهش حاضر بررسی اثر فعالیت ورزشی بر شدت بیماری، سطوح پروتئین NRF2 و آنزیم گزانتین اکسیداز در نخاع در دورۀ مزمن بیماری است.
روش پژوهش: 30 موش مادۀ C57Bl6 (6 -8 هفته) به سه گروه فعالیت ورزشی اختیاری، EAE و کنترل سالم تقسیم شدند. پس از القای EAE با میلین الیگودندروسیت گلیکوپروتئین (MOG35-55) و پس از آنکه موش‌ها دو روز پیاپی نمرۀ بالینی یک را نشان دادند، به مدت چهار هفته، پنج روز، روزی یک ساعت تمرین اختیاری را انجام دادند. 48 ساعت پس از آخرین جلسۀ تمرینی موش‌ها به‌وسیلۀ کتامین و زایلازین بی‌هوش شده و بافت نخاع برداشته شد. مکان‌یابی پروتئین‌های NRF2 در مادۀ خاکستری و سفید نخاع با استفاده از روش ایمونوهیستوشیمی بررسی شد و آنزیم گزانتین اکسیداز با استفاده از کیت گزانتین اکسیداز طبق دستورالعمل سازندۀ کیت به روش ELISA اندازه‌گیری شد.
یافته‌ها: فعالیت ورزشی اختیاری سطوح پروتئین NRF2 را در قسمت سفید (0.0001=P) و خاکستری نخاع افزایش (0.0001=P) و سطوح آنزیم زانتین اکسیداز در نخاع (0.001=P) و شدت بیماری را در دورۀ مزمن کاهش داد (0.01=P).
نتیجه‌گیری: فعالیت ورزشی اختیاری می‌تواند استرس اکسیداتیو و شدت بیماری را در دورۀ مزمن بیماری کاهش دهد و راهبرد مناسبی برای کنترل و درمان بیماری ام‌اس باشد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Voluntary Exercise Reduces Oxidative Stress and Disease Severity in Mice with Multiple Sclerosis
نویسندگان [English]
- Amirhossein Saffar Kohneh Quchan 1
- Mohammad kordi 2
- Fatemeh Shabkhiz 1
1 Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran.
2 Corresponding Author, Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran.
چکیده [English]
Introduction: Oxidative stress and the production of Reactive Nitrogen (RNS) and Oxygen (ROS) Species play an important role in the pathogenesis and the progression of MS. This study aimed to investigate the effect of exercise on disease severity, NRF2 protein levels, and xanthine oxidase enzyme in the spinal cord during the chronic period of the disease.
Methods: Thirty female C57Bl/6 mice (6-8 weeks) were divided into three Voluntary Exercise, Experimental Autoimmune Encephalomyelitis (EAE), and Healthy Control groups. After induction of EAE by Myelin Oligodendrocyte Glycoprotein (MOG35-55) and after the mice showed a clinical score of one for two consecutive days, they performed one hour of voluntary exercise for four weeks and five days a week. Forty-eight hours after the last training session, the mice were anesthetized with Ketamine and Xylazine, and the spinal cord tissue was removed. The location of NRF2 proteins in the gray and white matter of the spinal cord was investigated by Immunohistochemistry, and xanthine oxidase was measured by xanthine oxidase assay kit according to the kit manufacturer's instructions and by ELISA method.
Results: Voluntary exercise increased NRF2 protein levels in the white matter (P=0.0001) and gray matter (P=0.0001) of the spinal cord and decreased xanthine oxidase levels in the spinal cord (P=0.001), and attenuated disease severity during the chronic period (P=0.01).
Conclusion: Voluntary exercise reduces oxidative stress and disease severity during the chronic period of the disease and would be a good strategy for controlling and treatment of MS disease.
کلیدواژه‌ها [English]
- EAE
- Nrf2
- Voluntary wheel running
- Xanthine oxides
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