تأثیر بازتوانی قلبی بر نیمرخ چربی و بیان ژن ABCG8 در سلول‌های PBMC پس از CABG در مردان میانسال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد شیروان، دانشگاه آزاد اسلامی، شیروان، ایران

2 دانشیار فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران

3 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، پردیس بین المللی کیش، دانشگاه تهران، کیش، ایران

چکیده

ABCG8، پروتئین ناقل غشای سلولی است که موجب خروج کلسترول از سلول می‌شود. هدف از این تحقیق بررسی تأثیر یک دوره بازتوانی قلبی بر بیان ژن ABCG8 بود. 20 مرد میانسال (50 تا 60 سال) که تحت عمل جراحی CABG قرار گرفته بودند، در دو گروه کنترل (10 نفر) و تجربی (10 نفر) قرار گرفتند. تمرینات هوازی به مدت 8 هفته (3 جلسه در هفته) با شدتی معادل 11 تا 13 (شدت متوسط براساس مقیاس بورگ) تحت کنترل پزشک انجام گرفت. خون‌گیری پس از 12 تا 14 ساعت ناشتایی 48 ساعت قبل از شروع تمرینات و 48 ساعت پس از پایان برنامة تمرینی انجام گرفت. از روش RTPCR برای بررسی بیان ژن ABCG8 در هر دو گروه استفاده شد. تجزیه‌وتحلیل یافته‌ها با استفاده از آزمون Independent T-test نشان داد که در گروه تجربی بیان ژن ABCG8 در سلول‌های PBMN افزایش و نیمرخ لیپید بهبود یافت (05/0P≤). براساس یافته‌های تحقیق، بیان ژن ABCG8 پس از بازتوانی قلبی در سلول‌های PBMN یکی از سازوکارهای مربوط به انتقال معکوس کلسترول است که می‌تواند در پیشگیری از وقوع حملة مجدد قلبی پس از CABG مؤثر باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Cardiac Rehabilitation on Lipid Profile and ABCG8 Gene Expression in PBMC Cells after CABG in Middle-Aged Men

نویسندگان [English]

  • mohsen jafari 1
  • Amir Rashidlamir 2
  • Reza Nuri 3
1 Assistant Professor in Exercise Physiology, Department of Sport Sciences, Shirvan Branch, Islamic Azad University, Shirvan, Iran
2 Associate Professor in Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor in Exercise Physiology, Department of Sport Sciences, Kish International Campus, University of Tehran, Kish, Iran
چکیده [English]

ABCG8 is a cellular membrane transport protein that induces cholesterol efflux. The aim of this study was to investigate the effects of a period of cardiac rehabilitation on ABCG8 gene expression. 20 middle-aged men (50 to 60 years old) that experienced CABG, were assigned to two groups (control (n=10) and experimental (n=10)). Aerobic exercises were performed for 8 weeks (3 sessions per week) with the intensity of 11 to 13 (moderate intensity on the basis of Borg scale) under the control of a physician. Blood samples were collected after 12 to 14 hours of fasting 48 hours before and 48 hours after the exercises. RTPCR method was used to examine the gene expression of ABCG8 in both groups. Analysis of data using independent t test showed that ABCG8 gene expression in PBMN cells increased and lipid profile improved in experimental group (P≤0.05). According to research findings, ABCG8 gene expression after cardiac rehabilitation in PBMN cells is one of the mechanisms related to reverse cholesterol transport that can affect the prevention of recurrent myocardial infarction after CABG.

کلیدواژه‌ها [English]

  • ABCG8
  • cardiac surgery
  • lipid profile
  • mononuclear cell
  • rehabilitation
1. Jafari M, Jalali S. The Effect of Interval and Continuous Training on Liver Genes Expression Related to Reverse Cholesterol Transport. Sport Physiology & Management Investigations. 2020;12(2):75-89. (Persian)
2. Jafari M. Effects of high intensity interval training and low intensity continuous training on some elements of reverse cholesterol transport in male wistar rats after high fat diet. Iranian Journal of Physiology and Pharmacology. 2020;4(1):81-76. (Persian)
3. Jafari M. Effect of physical activity on prevention and treatment of atherosclerosis: focus on activity of ABCG5 and ABCG8 genes. Journal of Gorgan University of Medical Sciences. 2019;21(3). (Persian)
4. Jafari M, Rashidlamir A, Dastani M, Fathi M, Alavinya SE. The effect of cardiac rehabilitation on ApoA1 and ApoB in men with coronary heart disease (CHD) after coronary artery bypass graft (CABG). Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch. 2018;28(2):117-23. (Persian)
5. Dababneh EH, Saha S, Ostlundh L, Al-Rifai RH, Oulhaj A. Protocol: Impact of cardiac rehabilitation on mortality and morbidity in diabetic versus non-diabetic patients: protocol for a systematic review and meta-analysis. BMJ Open. 2021;11(4).
6. Nalini M, Najafi F, Saeidi M, Gerami F, Rashidi A, Ezati P. Changes in exercise capacity and modifiable risk factors after a comprehensive cardiac rehabilitation in patients with and without left ventricular systolic dysfunction. J Kermanshah Univ Med Sci. 2009;13(3):e79594. (Persian)
7. Afzalaghaiee E, Hadian M, Attarbashi Moghadam B, Tavakol K, Zandparsa A, Jalaei S, et al. The effects of cardiac rehabilitation on changes in the lipid profile of Iranian male and female with coronary artery disease refered to Emam Khomeini Hospital complex. Journal of modern rehabilitation. 2010;4(1):45-9. (Persian)
8. Back M, Cider A, Gillström J, Herlitz J. Physical activity in relation to cardiac risk markers in secondary prevention of coronary artery disease. International journal of cardiology. 2013;168(1):478-83.
9. Kubilius R, Jasiukevičienė L, Grižas V, Kubilienė L, Jakubsevičienė E, Vasiliauskas D. The impact of complex cardiac rehabilitation on manifestation of risk factors in patients with coronary heart disease. Medicina. 2012;48(3):24.
10. Ghanbari-Niaki A, Rahmati-Ahmadabad S, Zare-Kookandeh N. ABCG8 gene responses to 8 weeks treadmill running with or without Pistachia atlantica (Baneh) extraction in female rats. International journal of endocrinology and metabolism. 2012;10(4):604.
11. Meissner M, Nijstad N, Kuipers F, Tietge UJ. Voluntary exercise increases cholesterol efflux but not macrophage reverse cholesterol transport in vivo in mice. Nutrition & metabolism. 2010;7(1):1-6.
12. Pérez IP, Zapata MA, Cervantes CE, Jarabo RM, Grande C, Plaza R, et al. Cardiac rehabilitation programs improve metabolic parameters in patients with the metabolic syndrome and coronary heart disease. The Journal of Clinical Hypertension. 2010;12(5):374-9.
13. Gordon NF, Habib A, Salmon RD, Bishop KL, Drimmer A, Reid KS, et al. Effect of exercise-based cardiac rehabilitation on multiple atherosclerotic risk factors in patients taking antidepressant medication. The American journal of cardiology. 2013;111(3):346-51.
14. Abdi M, Marefati H, Moazenzadeh M. The effect of cardiac rehabilitation on the serum levels of adiponectin and lipoproteins in male atherosclerotic patients. Journal of Kerman University of Medical Sciences. 2012;19(4):317-25. (Persian)
15. Kajinami K, Brousseau ME, Nartsupha C, Ordovas JM, Schaefer EJ. ATP binding cassette transporter G5 and G8 genotypes and plasma lipoprotein levels before and after treatment with atorvastatin. Journal of lipid research. 2004;45(4):653-6.
16. Chan D, Watts G, Barrett P, Whitfield A, Van Bockxmeer F. ATP-binding cassette transporter G8 gene as a determinant of apolipoprotein B-100 kinetics in overweight men. Arteriosclerosis, thrombosis, and vascular biology. 2004;24(11):2188-91.
17. Mohammadi A, Mirzaei F, Moradi MN, Jamshidi M, Yari R, Ghiasvand T, et al. Effect of flaxseed on serum lipid profile and expression of NPC1L1, ABCG5 and ABCG8 genes in the intestine of diabetic rat. Avicenna Journal of Medical Biochemistry. 2013;1(1):1-6.
18. Butcher LR, Thomas A, Backx K, Roberts A, Webb R, Morris K. Low-intensity exercise exerts beneficial effects on plasma lipids via PPARγ. Medicine & Science in Sports & Exercise. 2008;40(7):1263-70.
19. Jalali S, Jafari M. Effects of High Intensity Interval (HIT) versus Continuous Trainings on ABCG5 and ABCG8 Genes Expression in Male Wistar Rats after High Fat Diet. Res Med. 2019;43(4):216-221. (Persian)
20. Jafari M, Ravasi AA. Effect of interval and continuous training exercises after high-fat diet on liver X receptor alpha gene expression. Tehran University Medical Journal TUMS Publications. 2020;78(1):28-32. (Persian)
21. Jafari M. eroxisome proliferator-activated receptors beta and gamma (PPARβ and PPARγ) genes expression following exercise trainings and high fat diet in male Wistar rats. Journal of Practical Studies of Biosciences in Sport. 2021;9(17):58-67. (Persian)