نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسنده
دانشیار گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه ولی عصر (عج)، رفسنجان، ایران
چکیده
آتروفی عضلانی از برجسته‌ترین مشخصه‌های بی‌تمرینی و همراه با کاهش قدرت و عملکرد عضله است. هدف پژوهش حاضر بررسی اثر خراش عصبی (CCI) پس از 6 هفته تمرین مقاومتی، استقامتی و ترکیبی بر مقدار پروتئین Tweak و Fn14 در عضلۀ کف پایی بود. 32 سر موش صحرایی نر به‌طور تصادفی به چهار گروه تقسیم شدند: 1. گروه کنترل-خراش عصبی(CCI-C) ، 2. گروه تمرین ترکیبی-خراش عصبی (CCI-CO)، 3. گروه تمرین استقامتی-خراش عصبیCCI-E)) و 4. گروه تمرین مقاومتی-خراش عصبی (CCI-S). گروه‌های تجربی به مدت 6 هفته و 4 روز در هفته در برنامۀ تمرینات استقامتی و مقاومتی مخصوص خود شرکت کردند. پس از 6 هفته تمرین، پروتکل کاهش فعالیت بدنی به شکل CCI به مدت 4 هفته اجرا شد. در پایان عضلۀ کف‌پایی استخراج و مقدار پروتئین‌ها به روش وسترن بلات سنجیده شد. برای تعیین تفاوت در مقدار پروتئین‌ها از آزمون تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در سطح معنا‌داری کمتر از 05/0 استفاده شد. نتایج نشان داد که میزان پروتئین Tweak و Fn14 در گروه‌های CCI-E ، CCI-S و CCI-CO نسبت به گروهCCI-C به‌طور معنا‌داری کمتر بود (05/0P<). همچنین، تفاوت معنا‌داری در پروتئین Tweak در گروه CCI-E با گروه‌های CCI-S و CCI-CO و میزان پروتئین Fn14 در گروه CCI-E با گروه CCI-CO مشاهده شد (05/0P<). با توجه به یافته‌های پژوهش حاضر و افزایش کمتر پروتئین‌های آتروفیک در موش‌هایی که برنامۀ تمرینی انجام دادند، می‌توان ادعا کرد انجام تمرینات ورزشی قبل از یک دوره کاهش فعالیت بدنی سبب کاهش افت عملکرد عضلانی پس از بی‌تمرینی خواهد شد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The Effect of the Chronic Constriction Injury on Tweak and Fn14 Protein in Plantaris Muscle of Wistar Rats after of Endurance, Resistance and Combined Training
نویسنده [English]
- Abdolreza Kazemi
Associate Prof, Dept of Physical Education, Faculty of Letters and Humanities, Vali E Asr University, Rafsanjan, Iran.
چکیده [English]
Decreasing muscle mass is one of the detraining characteristics that associated with attenuated in muscle strength and muscle performance, and resulting in disability in performing of daily tasks. The purpose of the present study was to investigate the effect of decreased physical activity in the form of chronic constriction injury (CCI) on the Tweak and Fn14 proteins after resistance, endurance and combined exercises. 32 rats were randomly divided into four groups: 1- Control group-CCI (Sham+CCI), 2- Mixed training group-CCI (Mix+CCI), 3- Endurance training group-CCI (TE+CCI), and 4- Resistance training group-CCI (LA+CCI). Mix+CCI, TE+CCI and CCI+SNL groups participated in training program for six weeks. After performing 6 weeks training, chronic constriction injury was implemented for four weeks. Western blotting technique was used to measure proteins. The results extracted by ANOVA and showed that Tweak and Fn14 proteins increased by CCI. The level of Tweak and Fn14 proteins in CCI+La and CCI+Mix groups and also CCI-TE group was significantly lower than Sham+CCI group (P <0.05). One the other hands, the level of Fn14 protein in CCI-LA compared with CCI-TE and CCI-MIX was not significant (P >0.05). According to the findings of the present study, it seems that mice that had resistance training in their training program experienced less atrophy than the group that did only endurance training.
کلیدواژه‌ها [English]
- : chronic constriction injury
- Plantaris Muscle
- Tweak
- Fn14
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