تأثیر هشت هفته تمرین تناوبی بر میزان پروتئین‌های کلوتو، پروتئولیپید پروتئین و فاکتور نکروزتوموری آلفا در بافت مخچة موش‌های سالم

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی دانشکدة علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران

2 دانشیار دانشکدة علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران

3 استادیار دانشگاه بهزیستی و توانبخشی، تهران، ایران

4 دانشکده تربیت بدنی و علوم ورزشی دانشگاه خوارزمی

5 استاد دانشگاه مموریال، سنت جانز، کانادا

چکیده

امروزه در تحقیقات بسیاری نشان داده شده است که تمرینات ورزشی به سلامت و بهبود عملکرد مغز منجر می‌شود. همچنین ممکن است مسیرهای عصبی و نورولوژیکی را در مغز افزایش دهند. هدف از پژوهش حاضر بررسی تأثیر ۸ هفته تمرین تناوبی با شدت ۸۰ درصد سرعت بیشینه در هفتة اول تا ۱۱۰ درصد سرعت بیشینه در هفتة آخر بر میزان کلوتو،  PLPو TNF-α بافت مخچة موش‌های سالم بود. بدین‌منظور، تعداد ۲۰ سر موش ماده به‌طور تصادفی به دو گروه تمرین (۱۰=n) و کنترل (۱۰=n) تقسیم شدند. در گروه تمرین، حیوانات ۸ هفته تمرین تناوبی بر تردمیل را اجرا کردند. ابتدا به مدت ۳ دقیقه با سرعت ۶ متر بر دقیقه دویدند و هر ۳ دقیقه یک‌بار، ۳ متر بر دقیقه به‌سرعت تردمیل اضافه شد تا زمانی‌که قادر به حفظ این شدت نباشند. آخرین تلاش هر موش به‌عنوان حداکثر سرعت درنظر گرفته شد. در نهایت، پروتکل تمرینی به مدت ۸ هفته ۵ جلسه در هفته اجرا شد. از روش وسترن بلات به‌منظور اندازه‌گیری متغیرهای تحقیق استفاده شد. داده‌ها با استفاده از آزمون t مستقل در سطح معناداری 05/0 P≤ تجزیه تحلیل شدند. نتایج تحقیق نشان داد، تمرین تناوبی به افزایش میزان کلوتو (01/0P≤) , PLP (05/0P≤) و کاهش میزان TNF-α (05/0P≤) در گروه تمرین در مقایسه با گروه کنترل منجر شد. در نهایت می‌توان گفت ۸ هفته تمرین تناوبی میزان پروتئین‌های میلین‌ساز را افزایش و میزان TNF-α را کاهش داد. به‌نظر می‌رسد تمرین ورزشی با افزایش در بیومارکرهای میلین‌ساز، نقش مهمی در حفاظت از سیستم اعصاب مرکزی دارد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of 8 weeks of high-intensity interval training on Klotho, PLP and TNF-α Cerebellum tissue mice

نویسندگان [English]

  • Fataneh Farahmand 1
  • Maryam Nourshahi 2
  • Maryam Soleimani 3
  • hamed rajabi 4
  • kevin power 5
1 Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran
2 Department of Medical Basic Sciences, University of Social Welfare and Rehabilitation Sciences Tehran, Iran,
3 Department of Exercise Physiology, Sport Science Faculty, Kharazmi University, Tehran, Iran
4 Department of Exercise Physiology, Sport Science Faculty, Kharazmi University, Tehran, Iran,
5 School of Human Kinetics and Recreation, Memorial University of Newfoundland, St. John’s, NL, Canada
چکیده [English]

Nowadays, many studies have shown that exercise improves brain health and performance and may also increase neurological pathways in the brain. The aim of the present study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) on Klotho, PLP and TNF-α concentrations in mice. Female mice were randomly divided to exercise (EX) or control (Con) groups. In the EX group, animals performed 8-week of HIIT. Mice initially ran at 6 m/min for 3 min at 0% grade, with the speed progressively increasing by 3 m/min every 3 min until exhaustion - when the mice were unable to maintain the required running speed. The maximal speed obtained was then used to calculate the individualized running speed for mice in the EX group. HIIT involved treadmill running 5 days per week for the final 6 weeks. Klotho, PLP and TNF-α expressions were studied using Western blot. Mice in the EX group had a significant increase in klotho and PLP concentrations compared to the control group (p≤0.01 and p≤0.05, respectively). Also, in the exercise groups mice had a significant decrease in TNF-α concentrations in compared to the control group (p≤0.05). Our data demonstrate that HIIT increased klotho and PLP and deceased neuroinflammatory cytokines. These proteins are associated with increasing myelination and protect the central nerve system against neurodegenerative diseases.

کلیدواژه‌ها [English]

  • PLP
  • interval training
  • TNF-α
  • Klotho
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