اثر پیشگیرانة شش هفته تمرین شنا بر سطوح برخی از سایتوکاین‌ها و عامل تنظیم نورونی مشتق‌شده از مغز در رت‌های مدل EAE

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دکتری تخصصی فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی دانشکدة علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 دانشیار، دکتری تخصصی فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی دانشکدة علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

3 دانشجوی دکتری تخصصی فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی دانشکدة علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

چکیده

مالتیپل اسکلروزیس یک بیماری خودایمنی پیش‌رونده و مزمن سیستم اعصاب مرکزی است که با از بین رفتن غلاف میلین سلول‌های عصبی همراه است. هدف از پژوهش حاضر، بررسی اثر پیشگیرانة 6 هفته تمرین شنای اجباری بر سطوح TNF-α، IL-10 و BDNF در رت‌های مدل EAE بود. 25 سر رت مادة نژاد لوئیس به پنج گروه شامل کنترل سالم، کنترل ام.اس، شنای سالم، شنای ام.اس و حلال تقسیم شدند. تمرینات شنا به مدت 6 هفته و 5 روز در هفته انجام گرفت. برنامة تمرین شنا با 30 دقیقه آغاز شد، که این مدت با افزایش پنج دقیقه روزانه در هفتة دوم به 60 دقیقه رسید. اضافه‌بار تمرینی با تنظیم قدرت و سرعت آب هنگام شنا، تنظیم شد. همچنین، مدل EAE در انتهای هفتة ششم تمرینات القا و پس از 14 روز بافت‌برداری از مغز رت‌ها انجام گرفت. نیمکرة راست برای بافت‌شناسی و نیمکرة چپ به‌منظور انجام آنالیز سنجش پروتئین به آزمایشگاه منتقل شد. تمرین شنا در رت‌های مدل EAE به کاهش معنا‌دار TNF-α P < 0/001 و عدم تغییر معنا‌داری در IL-10 (71/0=P) و BDNF (70/0=P) منجر شد. علائم بالینی در رت‌های تمرین‌کرده با تأخیر ظاهر شد. علاوه‌بر این، نتایج حاصل از رنگ‌آمیزی بافتی نشان داد که بین گروه تمرین کرده و تمرین‌نکرده تفاوت چشمگیری از نظر التهاب مشاهده نشد. دمیلینه شدن ناشی از مدل EAE سبب افزایش آسیب به بافت مغزی می‌شود که نشان می‌دهد ورزش شنا با شدت و مدت مطالعة حاضر نمی‌تواند در پیشگیری از بیماری مؤثر باشد، هرچند این علائم در مدت کوتاه به تأخیر افتاده باشد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Preventive effect six weeks of swimming training on the levels of cytokines and brain-derived neurotrophic rat model of EAE

نویسندگان [English]

  • sayd mojtaba hoseini 1
  • ziya falahmohammadi 2
  • vahid talebi 3
1 PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran
2 . Associate Professor PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran
3 PhD student of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran
چکیده [English]

Multiple sclerosis is a progressive and chronic autoimmune disease of the central nervous system that is associated with the destruction of the myelin sheath of nerve cells. The aim of this study was to investigate the preventive effect of six weeks of forced swimming on the levels of TNF-α, IL-10 and BDNF is the EAE model rats. Twenty-five female Lewis were divided into five groups: healthy control, MS control, healthy swimming, MS swimming, and solvent. Swimming training for 6 weeks and 5 days a week. Swimming training program began with 30 minutes daily five-minute period in the second week with an increase to 60 minutes. Overload training was set by adjusting the power and speed of the water while swimming. Also, EAE was induced model at the end of the sixth week of training and After 14 days’ mice were sacrificed. Right Cerebral hemisphere for the histology and right Cerebral hemisphere was transferred to the lab for analysis of protein measurements. Swimming exercise in rats EAE model leads to a significant reduction in TNF-α (P=0/001) and no significant change in IL-10 (P=0/71) and BDNF (P=0/70) was. In rats trained clinical signs appeared delayed. Furthermore, results of histological staining showed that certain differences were observed between trained and untrained. EAE-induced demyelination model increases the damage to brain tissue Shows that swimming exercise intensity and duration of six weeks can be effective in delaying symptoms, although these symptoms are delayed in the short term.

کلیدواژه‌ها [English]

  • experimental autoimmune encephalomyelitis
  • Lewis rats
  • swimming training
  • cytokine
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