نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دکتری تخصصی فیزیولوژی ورزشی گرایش عصبی – عضلانی دانشگاه تهران، تهران، ایران
2 دانشیار گروه فیزیولوژی ورزش، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
3 استادیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران
چکیده
هدف از پژوهش حاضر بررسی بیان ایزوفرم های PGC-1 آلفا در پاسخ به تمرینات مقاومتی اکسنتریک و کانسنتریک در افراد سالم بود.
روش تحقیق: 10 مرد سالم بصورت تصادفی در هر یک از گروه ها (کانسنتریک 5 نفر- اکسنتریک 5 نفر) تقسیم شدند. پروتکل های انقباض آیزوکینتیک شامل اکسنتریک و کانسنتریک اکستنشن زانو با حداکثر قدرت و سرعت زاویه ای 60 درجه بر ثانیه بود. گشتاورها برای هر آزمودنی به منظور همسان سازی بارکاری در هر دو پروتکل یکسان و سرعت رفت و برگشت 60 درجه بر ثانیه بود. انقباض ها شامل حدکثر 12 ست 10 تکراری برای پای راست، زمان استراحت بین هر ست 30 ثانیه در نظر گرفته شد. در ابتدا و انتهای مطالعه بافت عضله پهن جانبی بایوپسی شد. بایوپسی در دو جهت دیستال و پروگزیمال عضله پهن جانبی انجام شد. برای بررسی بیان ژن PGC1α-1 و PGC1α-4، در هر گروه بررسی بافت ها از تکنیک PCR Real Time استفاده شد. برای تجزیه و تحلیل داده‌های این پژوهش از روش آماری تی وابسته و آزمون کوواریانس استفاده شد.
یافته‌ها: تغییرات درون گروهی PGC1α-1 ، در گروه اکسنتریک (168/0p=) و کانسنتریک (959/0p=) معنادار نبود. همچنین تغییرات درون گروهی PGC1α-4 ، در گروه اکسنتریک (012/0p=) و گروه کانسنتریک (022/0p=) معنادار بود.
نتیجه‌گیری: در مجموع بنظر می رسد عدم تغییرات معنادار در متغیرهای مورد نظر ناشی از عدم فشار تمرینی کافی جهت تحریک افزایش PGC1α-1 و PGC1α-4 باشد. و با توجه به بررسی پاسخ بنظر می رسد در بحث سازگاری نتایج متفاوتی بدست آید که نیاز به بررسی دارد.
کلیدواژه‌ها
عنوان مقاله [English]
Expression of PGC-1 Alpha Isoforms in Response to Eccentric and Concentric Resistance Training in Healthy Subjects
نویسندگان [English]
- Pejman Taghi Beikzadeh Badr 1
- Fatemeh Shabkhiz 2
- Shahnaz Shahrbanian 3
1 PhD of Exercise Physiology, neuromuscular Branch, University of Tehran, Tehran, IranTehran university
2 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 . Assistant professor, Department of Sport Science, Faculty of Humanities, Tarbiat Modares University, Tehran, IRAN
چکیده [English]
The aim of this study was to investigate the expression of PGC-1 alpha isoforms in response to eccentric and concentric resistance exercise in healthy subjects.
Materials and Methods: Ten healthy men were randomly divided into two groups (5 men concentric and 5 men eccentric). Isokinetic contraction protocols included eccentric and concentric knee extension with maximum power and angular velocity of 60 degrees per second. The torques for each subject were 60 degrees per second to match the workload in both identical protocols and the rotational speed. Contractions consisted of a maximum of 12 sets of 10 repetitions for the right leg, a rest time of 30 seconds between each set. At the beginning and end of the study, lateralis muscle tissue was biopsied. Biopsies were performed in both distal and proximal directions of the lateral flank. Real time PCR was used to evaluate PGC1α-1 and PGC1α-4 gene expression in each tissue group. Data were analyzed using dependent t-test and covariance test.
Results: The intra-group changes of PGC1α-1 were not significant in eccentric (p = 0.168) and concentric (p = 0.959) groups. There was also a significant difference between PGC1α-4, eccentric group (p = 0.012) and concentric group (p = 0.02).
Conclusion: It seems that lack of significant changes in the desired variables due to the lack of exercise pressure is sufficient to stimulate the increase of PGC1α-1 and PGC1α-4. with regard to reviewing the answer, it seems that the compatibility debate has different results that need to be addressed.
کلیدواژه‌ها [English]
- "Eccentric contraction
- "Concentric contraction
- "PGC1α-4
- " PGC1α-1
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