تأثیر یک دوره تمرین استقامتی فزاینده بر شاخص‌های تنظیم‌کنندة آپوپتوز سرم موش‌های ویستار مبتلا به انفارکتوس قلبی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 .دانشجوی دکتری، گروه تربیت بدنی، واحد محلات، دانشگاه آزاد اسلامی، محلات، ایران

2 دانشیار، گروه تربیت بدنی، واحد محلات، دانشگاه آزاد اسلامی، محلات، ایران

3 استاد، گروه فیزیولوژی، دانشگاه علوم پزشکی اراک، اراک، ایران

4 دانشیار، گروه آسیب شناسی و فیزیولوژی ورزشی، دانشکدة علوم ورزشی، دانشگاه اراک، اراک، ایران

چکیده

بیماری‌های قلبی، به‌دلیل مزمن بودن، اغلب فعالیت‌های روزمرة فرد را تحت تأثیر قرار می‌دهند و علت اصلی مرگ‌ومیر در جهان است. هدف از این پژوهش بررسی شاخص‌های تنظیم‌کنندة آپوپتوز پس از یک دوره تمرین استقامتی فزاینده در سرم موش‌های ویستار مبتلا به انفارکتوس قلبی است. پژوهش حاضر از نوع تجربی است و به این منظور 20 سر موش صحرایی نژاد ویستار با محدودة سنی10-8 هفته و میانگین وزن 30±230 گرم به‌طور تصادفی به دو گروه کنترل انفارکتوس (Sed.MI) و تمرین انفارکتوس (Ex.MI) تقسیم شدند. پس از القای انفارکتوس قلبی با دو تزریق داخل‌صفاقی ایزوپروترنول به فاصلة 24 ساعت و با دوز 150 میلی‌گرم در کیلوگرم، گروه تمرین به مدت 8 هفته پروتکل تمرین استقامتی فزاینده را روی نوار گردان اجرا کردند. خون‌گیری در شرایط یکسان و در پی حداقل 24 ساعت پس از آخرین جلسة تمرین انجام گرفت و از روش الایزا برای سنجش متغیرهای مطالعه استفاده شد. برای بررسی تغییرات معنا‌داری سطح کاسپاز-8، سیتوکروم-C و کاسپاز-3 از روش آنالیز تی مستقل در برنامة گراف پد استفاده شد. نتایج نشان داد سطوح سرمی کاسپاز-8، سیتوکروم- C و کاسپاز-3 در گروه Ex.MI تغییراتی نسبت به گروه Sed.MI داشته است. این تغییرات در مقادیر کاسپاز-3 (65/0=P) و سیتوکروم-C (25/0=P) پلاسمایی بین دو گروه Sed.MI و Ex.MI معنا‌دار نبود؛ اما تغییرات در مقادیر کاسپاز-8 تفاوت معنا‌داری را نشان داد (04/0=P). براساس نتایج پژوهش حاضر به‌نظر می‌رسد به‌کارگیری راهبردهای غیردارویی مانند تمرین استقامتی فزاینده نمی‌تواند میزان آپوپتوز را در نمونه‌های مبتلا به انفارکتوس قلبی بهبود دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of apoptotic regulator markers after a period of increased endurance training in serum of Wistar rats with MI

نویسندگان [English]

  • mojtaba khansooz 1
  • bahram abedi 2
  • mohammad reza palizvan 3
  • Abbas Saremi 4
1 Ph.D. Student, Department of Physical Education, Mahallat Branch, Islamic Azad University Mahallat, Iran
2 . Associate Professor, Department of Physical Education, Mahallat Branch, Islamic Azad University Mahallat, Iran
3 . professor, Department of Physiology, Arak University of Medical Sciences, Arak, Iran
4 Associate Professor, Department of Exercise Physiology, Faculty of Sport Sciences, Arak University, Arak, Iran
چکیده [English]

Aim: Cardiovascular disease, due to its chronicity, often affects everyday activities of the individual and is the leading cause of death in the world. The purpose of this study was to investigate the effect of increasing endurance training on adjustment indicators Apoptosis is indicated in samples of patients with myocardial infarction.
Methodology: In this study, 20 Wistar rats aged 8-10 weeks with a mean weight of 230±30 for two groups of infarction control (Sed.MI) and infarction (Ex.MI). After induction of experimental myocardial infarction with two intraperitoneal isoproterenol injections at a dose of 150 mg/kg for 24 hours, the exercise group performed an endurance training protocol on the treadmill for eight weeks. Applying in the same conditions followed at least 24 hours after the last training session. To examine the significant changes in the levels of Caspase-3, Caspase-8 and Cytochrome-C, independent t-test was used in Graph Pad program.
Results: The results showed that serum levels of Caspase-8, cytochrome-C and Caspase 3 in Ex.MI group were higher than that of Sed.MI group. This increase was not significant in the cases of Caspase-3(p=0.65) and cytochrome-c (p=0.25) between Sed.MI and Ex.MI. But increase in caspase-8 values showed significant differences (p=0.04).
Conclusion: According to the results of this study, it seems that application of non-pharmacological strategies such as incremental endurance training cannot improve apoptosis rate in samples with myocardial infarction.

کلیدواژه‌ها [English]

  • Experimental Cardiac Infarction
  • Endurance training
  • Cytochrome-C
  • caspase-3
  • Caspase-8
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