اثر چاقی و تمرین استقامتی شدید متعاقب آن بر غلظت آیریزین سرم و بیان UCP1 mRNA چربی زیرپوستی و ارتباط این عوامل با تغییرات لپتین در موش‌های نژاد ویستار

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران

2 استاد، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران

3 دانشیار، گروه فارماکولوژی، مرکز تحقیقات سلولی و مولکولی دانشکده پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران

چکیده

اطلاعات درباره­ی غلظت و تنظیم آیریزین تحت شرایط فیزیولوژیکی مختلف، کم است. هجده سر موش، 12 هفته غذای پرچرب و 6 سر غذای استاندارد (غیرچاق) مصرف کردند. سپس گروه غیرچاق و 6 سر موش چاق (چاق پایه) کشته و دوازده سر موش باقی­مانده به گروهای چاق تمرین و چاق کنترل تقسیم شدند. گروه تمرینی 14 هفته، هر هفته 5 روز با شدت بالا روی نوارگردان دویدند. نمونه­ها 48 ساعت پس از آخرین جلسه­ی تمرینی گرفته شد. غلظت آیریزین و لپتین سرم در گروه چاق پایه بالاتر از گروه غیرچاق بود (05/0≥P). تمرین منجربه افزایش آیریزین و کاهش لپتین سرم گردید (05/0≥P). بیان UCP1 mRNA در گروه چاق پایه کمتر از گروه غیرچاق بود (05/0≥P). درحالی­که در گروه تمرینی نسبت به گروه کنترل افزایش داشت (05/0≥P). ارتباط لپتین و آیریزین در گروه چاق پایه مثبت و گروه تمرینی منفی بود (05/0≥P). لپتین و UCP1 در گروه چاق پایه ارتباط منفی داشتند (05/0≥P). علیرغم اینکه چاقی با افزایش لپتین و آیریزین همراه بود، اما بین لپتین وUCP1  یک ارتباط منفی معنی‌دار مشاهده شد. بااین­حال، تمرین با کاهش لپتین و افزایش آیریزین و UCP1  سبب کاهش وزن موش‌های چاق گردید.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Obesity and Its Subsequent Intense Endurance Training on Serum Irisin Concentration and Expression of Subcutaneous Adipose Tissue UCP1 mRNA and These Factors' Relation with Leptin Changes in Wistar Rats

نویسندگان [English]

  • Mahbanou Ghaderi 1
  • Hamid Mohebbi 2
  • Bahram Soltani 3
1 PhD Student in Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
2 Professor, Department of Exercise Physiology, Faculty of Education and Sport Sciences, University of Guilan, Guilan, I.R. Iran
3 Associate professor, Department of Pharmacology, Research Center for Molecular and Cellular Faculty of Medicine, Guilan University of Medical Sciences, Guilan, I.R. Iran
چکیده [English]

There are little data on irisin concentration and regulation under various physiological conditions. 18 rats had a high-fat diet and 6 rats were nourished with a standard diet (non-obese) for 12 weeks. Then, non-obese group and 6 obese rats (baseline obese) were killed. 12 remaining rats were divided into training obese and control obese groups. Training group ran on a treadmill for 14 weeks, 5 days/week with high intensity. Samples were collected 48 hours after last training session. Serum irisin and leptin concentrations in baseline obese group were higher than non-obese group (P≤0.05). Training increased serum irisin and decreased serum leptin (P≤0.05). In the baseline obese group, UCP1 mRNA expression was lower than non-obese group (P≤0.05) while it was more in training group than control group (P≤0.05). There was a positive relationship between irisin and leptin in baseline obese group and a negative relationship in the training group (P≤0.05). There was a negative relationship between leptin and UCP1 in baseline obese group (P≤0.05). Although the obesity was associated with an increase in leptin and irisin, there was a negative significant relationship between leptin and UCP1. However, training led to weight loss of obese rats by leptin decrease and irisin and UCP1 increase.

کلیدواژه‌ها [English]

  • آیریزین
  • تمرین استقامتی شدید
  • چاقی
  • لپتین
  • UCP1 mRNA

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