Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 PhD Student in Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran

2 Professor, Department of Exercise Physiology, Faculty of Education and Sport Sciences, University of Guilan, Guilan, I.R. Iran

3 Associate professor, Department of Pharmacology, Research Center for Molecular and Cellular Faculty of Medicine, Guilan University of Medical Sciences, Guilan, I.R. Iran


There are little data on irisin concentration and regulation under various physiological conditions. 18 rats had a high-fat diet and 6 rats were nourished with a standard diet (non-obese) for 12 weeks. Then, non-obese group and 6 obese rats (baseline obese) were killed. 12 remaining rats were divided into training obese and control obese groups. Training group ran on a treadmill for 14 weeks, 5 days/week with high intensity. Samples were collected 48 hours after last training session. Serum irisin and leptin concentrations in baseline obese group were higher than non-obese group (P≤0.05). Training increased serum irisin and decreased serum leptin (P≤0.05). In the baseline obese group, UCP1 mRNA expression was lower than non-obese group (P≤0.05) while it was more in training group than control group (P≤0.05). There was a positive relationship between irisin and leptin in baseline obese group and a negative relationship in the training group (P≤0.05). There was a negative relationship between leptin and UCP1 in baseline obese group (P≤0.05). Although the obesity was associated with an increase in leptin and irisin, there was a negative significant relationship between leptin and UCP1. However, training led to weight loss of obese rats by leptin decrease and irisin and UCP1 increase.


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