تأثیر تمرین تناوبی با شدت بالا و تمرین تداومی با شدت متوسط بر محتوای میتوکندری و PGC-1α چربی زیرپوستی در رت‌های نر چاق‌شده با رژیم غذایی پرچرب

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران

2 استاد، گروه فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران

3 استادیار، مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی تبریز، تبریز، ایران

چکیده

هدف پژوهش حاضر، بررسی آثار تمرینات تناوبی با شدت بالا (HIIT) و تداومی با شدت متوسط (MICT)بر محتوای میتوکندری و PGC-1α چربی زیرپوستی در رت‌های نر چاق بود. 40 سر رت نر پس از القای چاقی با رژیم غذایی پرچرب (به مدت 10 هفته)، 8 سر رت از گروه رژیم غذایی پرچرب (O) و 8 سر رت گروه رژیم غذایی استاندارد (C) کشته شده و سایر رت‌های چاق به‌طور تصادفی به سه گروه کنترل چاق (OC)، تمرین تداومی با شدت متوسط (MICT) و تمرین تناوبی با شدت بالا (HIIT) تقسیم شدند. برنامۀ HIIT شامل اجرای 10 وهله‌ فعالیت 4 دقیقه‌ای با شدت معادل 90-85 درصد VO2max و با دوره‌های استراحتی فعال 2 دقیقه‌ای و برنامۀ MICT با شدت معادل 70-65 درصد VO2maxبا مسافت طی‌شدۀ همسان با HIIT به مدت 12 هفته و 5 جلسه در هر هفته اجرا شدند. از روش وسترن بلات برای اندازه‌گیری PGC-1α و از روش RT-PCR برای اندازه‌گیری بیان ژن mtDNA استفاده شد. تحلیل داده­ها با استفاده از آزمون‌های ANOVA، ANCOVA و بونفرونی انجام گرفت و سطح معنا‌داری 05/0P≤ در نظر گرفته شد. نتایج تحلیل داده‌ها نشان داد که HIIT و MICT به افزایش شایان ‌توجه محتوی پروتئینی PGC-1α و بیان mtDNA منجر شدند (05/0P<) بااین‌حال، HIIT اثرات بیشتری داشت (05/0P<). به‌طور کلی، به‌نظر می‌رسد که HIIT و MICT به افزایش بیوژنز میتوکندری در چربی زیرپوستی منجر می‌شوند، درحالی‌که اثرات HIIT به‌طور چشمگیری بیشتر است

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of High Intensity Interval training and Moderate Intensity Continuous Training on Mitochondrial Content and PGC-1α of Subcutaneous Adipose Tissue in Male Rats with High Fat Diet Induced Obesity

نویسندگان [English]

  • mousa khalafi 1
  • Hamid Mohebbi 2
  • Pouran Karimi 3
  • Mehrsa Faridnia 3
  • Elma Tabari 1
1 PhD Student, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
3 Assistant Professor, Neuroscience Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran
چکیده [English]

The aim of this study was to investigate the effects of high intensity interval training (HIIT) and moderate intensity continuous training (MICT) on mitochondrial content and PGC-1α of subcutaneous adipose tissue in obese male rats. 40 male rats after inducing obesity with high fat diet (for 10 weeks), 8 rats from the high-fat diet group (O) and 8 rats of the standard diet group (C) were sacrificed and other obese rats were randomly divided into 3 groups: obesity control (OC), moderate intensity continuous training (MICT) and high intensity interval training (HIIT). The HIIT protocol included 10 bouts of 4-min. activity with the intensity equivalent to 85-90% vo2max and 2-min. active rest intervals. MICT protocol was carried out with the intensity of 65-70% VO2max with a covered distance similar to HIIT protocol for 12 weeks and 5 sessions per week. Western Blot method was used to measure PGC-1α and RT-PCR method was applied to measure mtDNA gene expression. Data were analyzed using ANOVA, ANCOVA and Bonferroni tests. The level of significance was considered as P≤0.05. The results of data analysis showed that HIIT and MICT drastically increased protein contents of PGC-1α and mtDNA expression (P<0.05). However, HIIT had more effects (P<0.05). Generally, it seems that HIIT and MICT increase mitochondrial biogenesis in subcutaneous adipose tissue while the effects of HIIT are drastically higher.

کلیدواژه‌ها [English]

  • high intensity interval training
  • insulin resistance
  • mitochondrial biogenesis
  • moderate intensity continuous training
  • Obesity
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