اثر مکمل یاری ویتامین D بر سطح کلوتو مغز در موش‌های صحرایی ماده نژاد لوئیز به دنبال شش هفته فعالیت ورزشی شنا

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده علوم انسانی دانشگاه آزاد اسلامی واحد ساری ، ایران

2 دانشیار، گروه فیزیولوژی ورزش دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه مازندران، بابلسر، ایران

چکیده

هدف از اجرای این پژوهش، بررسی اثر 6هفته شنای استقامتی همراه مصرف مکمل ویتامینD بر سطح کلوتو بافت مغز بود.در این مطالعه تجربی، 25 موش‌ صحرایی به 5گروه: 1-کنترل سالم، 2- حلال، 3-ویتامین دی 4-شنا، 5-ترکیب ویتامین Dوتمرین شنا ، تقسیم شدند. برنامه تمرینی شنا به مدت 6 هفته و 5 روز در هفته، و هرروز به مدت یک ساعت بود.گروه مکمل هم به مدت 2 هفته، مقدار 2 نانوگرم ویتامینD در2/0 میلی‌لیتر روغن کنجد، یک روزدرمیان به صورت درون صفاقی دریافت کردند. تجزیه و تحلیل داده‌ها با استفاده از آزمونone way ANOVA و آزمون تعقیبی Tukey و در سطح پنج درصد صورت گرفت. سطح کلوتو به روش الایزا اندازه‌گیری شد.نتایج نشان داد که بین سطح کلوتو بافت مغز گروه کنترل با گروه مکمل تفاوت معنی‌داری وجود نداشت (p=0/46).تفاوت میانگین بین گروه کنترل با گروه شنا (P=0.03) و ویتامین D+ شنا معنی‌دار بود (P=0.008). نتایج تحقیق حاضرنشان داد که تمرین شنا به تنهایی و در ترکیب با مصرف مکمل ویتامین Dمی تواند باعث افزایش سطح کلوتو بافت مغز شود و در نتیجه می تواند احتمالا نقش محافظتی در برابر عوامل تضعیف کننده سلامت دستگاه عصبی داشته باشد و منجر به افزایش قابل توجه ذخایر آن و نهایتا حفاظت نورونی گردد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of vitamin D supplementation on the levels of Klotho in the brain tissue of female lewis rats after 6 weeks of swimming

نویسندگان [English]

  • seyedeh fatemeh fatemi 1
  • ziya fallahmohammadi 2
1 PhD student of exercise physiology, Islamic Azad University , Sari Branch , Iran
2 .Phd, Associate Professor Faculty of Physical Education and Sport Sciences, University of Mazandaran Babolsar, Iran
چکیده [English]

The aim of this study was to investigate the effect of 6 weeks endurance swimming and vitamin D supplementation on Klotho levels of the brain tissue. In this study, 25 rats were divided into 5 groups: 1) healthy control 2) vehicle 3) vitamin D, 4) swimming and 5) vitamin D + swimming. Swimming training program included one hour daily for 6 weeks and 5 days per week. In addition, supplementary groups were received vitamin D supplement (2ng/0.2mg sesame oil/every other day) intraperitoneally for 2 weeks. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey post hoc test. Klotho levels were measured by ELISA. There was no significant difference in Klotho levels of brain tissue between control and vitamin D groups (p=0/46).The differences between swimming (P=0.03) and vitamin D +swimming (P=0.008) was significant. The results of this study showed that swimming alone and in combination with vitamin D supplementation could increase the Klotho levels of brain tissue and consequently may have neuroprotective role against detrimental factors and leads to a significant increase in resourcesand and finally neuronal protection.

کلیدواژه‌ها [English]

  • Brain
  • Klotho
  • swimming
  • vitamin D

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