نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزش دانشگاه تربیت مدرس، تهران، ایران
2 ۲. دانشیار گروه فیزیولوژی ورزش دانشگاه تربیت مدرس، تهران، ایران
3 دانشیار گروه ژنتیک پزشکی دانشگاه تربیت مدرس، تهران، ایران
4 استادیار گروه فیزیولوژی ورزش دانشگاه تربیت مدرس، تهران، ایران
چکیده
هدف از مطالعه حاضر، بررسی تاثیر یک دوره فعالیت ورزشی تناوبی همراه با نانوذرات سلنیوم بر بیان فاکتورهای اتوفاژی در موش های مبتلا به سرطان پستان بوده است. بدین منظور تعداد 64 سر موش بالب سی با دامنه ی سنی 3 تا 5 هفته به شکل تصادفی به گروه های کنترل، تمرین اینتروال، نانوذرات سلنیوم و تمرین اینتروال همراه با مصرف نانوذرات سلنیوم تقسیم شدند. قبل از سرطانی کردن، 6 هفته تمرین به عنوان اثر پیشگیری کننده و آماده سازی بر روی موش ها اعمال گردید و بعد از آن برای القا سرطان از رده سلولی 4T1 و پروتکل تمرین تناوبی و مصرف نانوذرات سلنیوم به مدت 6 پس از القا هفته انجام شد. یافته های تحقیق با استفاده از آزمون تحلیل واریانس سه سویه آنالیز شد، تاثیر سرطان بر بیان mTOR و تاثیر تمرین بر LC3 معنی دار بوده است. با توجه به نتایج به دست آمده از مطالعه حاضر مصرف نانوذرات سلنیوم نتوانسته به تنهایی از آتروفی عضلانی ممانعت کند با این وجود مصرف نانوذرات سلنیوم در کنار تمرینات ورزشی تا حد زیادی توانسته از آتروفی عضلانی جلوگیری نماید. نهایتاً بر اساس این نتایج سازگاری در ژن های اتوفاژی احتمالا یک تقابل جبرانی برای تعدیل هموستاز بدن است.
کلیدواژه‌ها
عنوان مقاله [English]
The Effect of Interval Training and Selenium Nanoparticles on mTOR and LC3 mRNA Expression in Mice Bearing Breast Cancer
نویسندگان [English]
- Kia Ranjbar 1
- Hamid Agha-Alinejad 2
- Shirin Shahbazi 3
- Mahdiyeh Molanouri Shamsi 4
1 PhD of exercise Physiology, faculty of human science, University of Tarbiat modares, Tehran, Iran
2 . associate professor of exercise Physiology, faculty of human science, University of Tarbiat modares, Tehran, Iran
3 . associate professor of medical genetics, faculty of medical science, University of Tarbiat modares, Tehran, Iran
4 assistant professor of Tarbiat Modares University
چکیده [English]
The aim of this study was to determine the effects of aerobic interval training and the purported antioxidant treatment, selenium nanoparticle supplementation on the expression of autophagy in 4T1 breast cancer-bearing mice with cachexia. 3-5 weeks old Balb/c mice were initially assigned to control, interval training, selenium nanoparticles and interval training-selenium nanoparticles (n = 15–16/group). After 6 weeks of training, half of each group was injected with 4T1 tumor cells, followed by 6 additional weeks of training. Finally, the mice were sacrificed, tissues were removed and were quantified using the Real-time PCR method. The effect of exercise training on the expression of LC3 and the effect of cancer on mTOR is significant. Combination of interval training and selenium nanoparticles maintained skeletal muscle function in cancer (P<0.05). finally, the adaptation of autophagic genes against physiological and pathologic stresses can be considered to be a counterbalance in body effort to modify these conditions and to bring the body to physiological homeostasis.
کلیدواژه‌ها [English]
- cancer
- exercise
- selinume nanoparticles
- autophagy
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