نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزش،گروه تربیت بدنی، واحدبروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
2 استادیار فیزیولوزی ورزش،گروه تربیت بدنی،واحد بروجرد،دانشگاه آزاد اسلامی،بروجرد،ایران
3 دانشیار فیزیولوزی ورزش،گروه تربیت بدنی،واحد بروجرد،دانشگاه آزاد اسلامی،بروجرد،ایران
4 دانشیار فیزیولوزی ورزش، دانشکده علوم ورزشی،دانشگاه بوعلی سینا،همدان،ایران
چکیده
تغییرات اپی‌ژنتیک و تجدید ساختار قلب از مهم‌ترین سازگاری‌های تمرینات استقامتی است. هدف مطالعه بررسی میزان بیان ژن هیستون‌داستیلاز4 و عامل افزایش‌دهندۀ مایوسیت2c بطن چپ موش‌های نر صحرایی در تعامل با شنای وامانده‌ساز است. بدین‌منظور 12 سر موش نر نژاد صحرایی با میانگین سنی 1±7 هفته و وزن 25±275 گرم به‌صورت تصادفی به دو گروه شش‌تایی کنترل و تمرین تقسیم شدند. پس از اجرای شنای وامانده‌ساز به مدت سه ساعت در هر جلسه و پنج روز در هفته به مدت ده هفته، بافت بطن چپ قلب آنها جدا شده، سپس بیان ژن به روش Real Time-PCR بررسی شد. داده‌ها توسط آزمون تی مستقل تحلیل و سطح معنا‌داری 05/0 P≤ انتخاب شد. نتایج نشان داد که پس از 10 هفته تمرین شنای وامانده‌ساز در مقایسه با گروه کنترل، میزان ژن هیستون‌داستیلاز4 افزایش (02/0P=)، اما میزان ژن عامل‌افزایش‌دهندۀ مایوسیت2c کاهش معنا‌داری (001/0P=) یافت. بنابراین، اجرای 10 هفته شنای شدید وامانده‌ساز از طریق افزایش فعالیت فاکتورهای رونویسی متصل به هیستون‌داستیلاز4، به افزایش میزان بیان این ژن منجر شد، و در پی آن بیان ژن عامل‌افزایش‌دهندۀ مایوسیت2c را کاهش داد.
کلیدواژه‌ها
عنوان مقاله [English]
The Effect of 10 Weeks of Exhaustive Swimming on Gene Expression of Histone Deacetylase-4 and Myocyte Enhancer Factor-2c in Left Ventricle in Male Rats
نویسندگان [English]
- Parivash Piraki 1
- Ahmad Hematfar 2
- Naser Behpour 3
- Mohamad Ali Samavati Sharif 4
1 Sport Physiology Student, Department of Physical Education, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
2 Assistant Professor in Sport Physiology, Department of Physical Education, Boroujerd Branch, Islamic Azad University, Boroujerd, Ira
3 Associate Professor in Sport Physiology, Department of Physical Education,Boroujerd Branch,Islamic Azad University,Boroujerd,Iran
4 Associate Professor in Sport Physiology,Sport Science Faculty,Buali Sina University,Hamadan,Iran
چکیده [English]
Epigenetic changes and cardiac reconstruction are among the most important adaptations in endurance training. The aim of this study was to investigate the gene expression of histone deacetylase-4 and myocyte enhancer factor-2c (MEF-2c) in male rats in interaction with exhaustive swimming. 12 male Wistar rats (mean age: 7±1 weeks, weight 275±25 gr) were randomly divided into two groups (each group 6 subjects): control and training. After performing the exhaustive swimming for 3 hours per session and 5 days per week for 10 weeks, their left ventricle was isolated; then the gene expression were investigated by Real Time-PCR. Data were analyzed by independent t test and the significance level was P≤0.05. The results showed that after 10 weeks of exhaustive swimming, the HDAC4 gene significantly increased (P=0.02), but MEF-2c gene significantly decreased (P=0.001) compared with the control group. Therefore, 10 weeks of exhaustive swimming increased gene expression of HDAC-4 and consequently reduced gene expression of MEF-2c by an increase in the activity of transcription factors attached to HDAC-4.
کلیدواژه‌ها [English]
- Exhaustive swimming
- histone deacetylase-4
- myocyte enhancer factor-2c
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