تاثیر8 هفته فعالیت هوازی و تناوبی شدید بر بیان ژن SOD و GPX بافت قلب موش‌های صحرایی نر ویستار

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس‌ارشد فیزیولوژی ورزشی دانشگاه پیام نور، تهران-ایران

2 . دانشیار گروه فیزیولوژی ورزشی دانشگاه پیام نور، تهران-ایران

3 استادیار گروه فیزیولوژی ورزشی دانشگاه پیام نور، تهران-ایران

چکیده

تمرین و فعالیت ورزشی علاوه بر فواید، عوارضی نیز دارندکه باید کنترل شود و افزایش فشار اکسایشی یکی از آنها می باشد. لذا در این مطالعه به بررسی سطح سازگاری دستگاه دفاع آنتی اکسیدانی در بافت قلب موش های ویستار پس از تمرینات تداومی و تناوبی پرشدت می پردازیم. بدین منظور تعداد 18 سر رت نر ویستار در یک دوره تمرینات ورزشی 8 هفته ای قرار گرفتند. 48 ساعت پس از آخرین جلسه تمرینی نمونه برداری خون و بافت قلب انجام شد. سپس مقادیر بیان ژن SOD و GPX بافت قلب با روش RT-PCR تعیین گردید. با توجه به غیرطبیعی بودن توزیع مقادیر بیان ژن در برخی گروه ها، از روش آماری کروسکال واریس برای تحلیل آماری استفاده شد. نتایج پژوهش نشان داد که میزان بیان ژن SOD در هر دو گروه تمرین تناوبی و استقامتی نسبت به گروه کنترل افزایش معناداری داشت. اما میزان بیان ژن GPX تنها در گروه تمرین HIT افزایش معناداری داشت. لذا تمرینات ورزشی تغییرات مطلوبی در سیستم دفاع آنتی اکسیدانی بافت قلب ایجاد می کند. اما بنظر می رسد که تمرینات تناوبی پرشدت تاثیرات مطلوب تری بر دفاع آنتی اکسیدانی داشته باشند

کلیدواژه‌ها


عنوان مقاله [English]

Effect of 8 weeks of high intensity intermittent and aerobic training on gene expression of SOD and GPX of heart tissue in Wistar male rats

نویسندگان [English]

  • Mahsa Dehghan Manshadi 1
  • MohammadReza Asad 2
  • Saeed Naghibi 3
1 MSc of Exercise Physiology, Payame Noor University, Tehran, Iran
2 Associate Professor, Department of Exercise Physiology, Payame Noor University, Tehran, Iran
3 Assistant Professor, Department of Exercise Physiology, Payame Noor University, Tehran, Iran
چکیده [English]

Exercise and physical activity have many benefits to improve the health, prevention, and treatment of diseases. But exercise also accuse complications should be controlled and increased oxidative stress is one of them. The aim of this study was to examine the compatibility of the devices in heart tissue antioxidant defense Wistar rats after continuous and intermittent exercise extreme here. A total of 18 male Wistar rats in the third group of high-intensity intermittent exercise (6 rats), exercise (6 rats) and control group (6 rats) were randomly assigned in a period of 8 weeks of exercise. 48 hours after the last training session, blood and tissue samples were collected by heart. The values of SOD and GPX heart tissue gene expression was determined by RT-PCR. Statistical analysis was performed using SPSS software. The results showed that SOD gene expression in both periodic and endurance training group and the control group was significantly increased. But the GPX gene expression was significantly increased only in HIT training.Exercise provides favorable changes in heart tissue and antioxidant defense system. These effects in both continuous and intermittent exercise extreme were observed. But it seems that interval training has extreme effects on antioxidant defense better.

کلیدواژه‌ها [English]

  • high-intensity intermittent exercise
  • aerobic exercise
  • antioxidant system
  • glutathione peroxide
  • superoxide dismutase

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