اثر تمرین هوازی و محدودیت کالری بر سطوح پروتئین ناقل گلوکز و دی آسیل گلسیرول درون‌عضلانی موش‌های صحرایی نر چاق

نوع مقاله: مقاله پژوهشی

نویسندگان

1 .دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزش، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه گیلان، ایران

2 دکتری تخصصی، استاد، گروه فیزیولوژی ورزش، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه گیلان، ایران

چکیده

مقاومت به انسولین ناشی از مصرف مواد غذایی پرچرب، با متابولیت‌های چربی درون‌عضلانی ارتباط دارد. هدف از مطالعۀ حاضر، بررسی اثر تمرین هوازی، محدودیت رژیم غذایی و ترکیب آنها بر مقاومت به انسولین از طریق تغییرات دی آسیل گلسیرول درون‌عضلانی در موش‌های صحرایی نر چاق بود. از 56 سر موش صحرایی نر نژاد ویستار با میانگین وزن 6/12±5/194 گرم به‌عنوان نمونه استفاده شد. 48 سر موش به مدت 18 هفته رژیم غذایی پرچرب مصرف کردند و به 8 سر موش غذای نرمال داده شد. سپس، موش‌های تحت رژیم غذایی پرچرب، به گروه­های کنترل، تمرین هوازی، محدودیت رژیم غذایی و ترکیبی (تمرین هوازی و محدودیت رژیم غذایی) تقسیم شدند. گروه تمرین هوازی به مدت 10 هفته و با شدت 28 متر بر دقیقه دویدند. گروه محدودیت رژیم غذایی 25 درصد از غذای مصرفی‌شان کاسته شد و گروه ترکیبی، به‌صورت یک روز در میان تحت تمرین هوازی و محدودیت رژیم غذایی قرار گرفتند. نتایج مطالعۀ حاضر نشان داد که وزن موش‌ها پس از 18 هفته مصرف رژیم غذایی پرچرب افزایش یافت (05/0>P). همچنین، تفاوت معنا‌داری بین سطوح دی آسیل گلیسرول عضله و سطوح سرمی انسولین، گلوکز و شاخص مقاومت به انسولین گروه‌های تمرین هوازی، محدودیت کالری و ترکیبی با گروه غذای پرچرب وجود دارد (05/0>P). سطوح PKC-θ در گروه‌های تعادل منفی انرژی کاهش یافت (05/0>P) و تفاوت معنا­داری بین سطوح GLUT4 گروه‌های تمرین هوازی و ترکیبی در مقایسه با گروه غذای پرچرب، مشاهده شد (05/0>P). نتایج این مطالعه نشان داد که روش­های تعادل منفی انرژی حتی همراه با مصرف غذای پرچرب می­تواند سبب کاهش دی آسیل گلیسرول عضلۀ اسکلتی شود که تا حد زیادی با بهبود مقاومت به انسولین همسوست.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Aerobic Exercise and Calorie Restriction on Intramuscular GLUT4 and DAG Levels in Obese Male Rats

نویسندگان [English]

  • Aboozar Jorbonian 1
  • Hamid Mohebbi 2
1 PhD Student of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Iran
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Iran
چکیده [English]

Insulin resistance induced by a high fat diet has been associated with intramuscular lipid metabolites. The aim of this study was to investigate the effect of aerobic exercise, diet restriction and their combination on insulin resistance through intramuscular diacylglycerol changes in obese male rats. Sample consisted of 56 male Wistar rats (mean weight: 194.5±12.6 g). 48 rats had a high-fat diet for 18 weeks and 8 rats had normal diet. Then, high-fat diet rats were divided into control (C), aerobic Exercise (E), caloric restriction (CR), and a combination (aerobic Exercise + caloric restriction (E+C) groups. E group ran on a treadmill at 28 m. per minute for 10 weeks. In CR group, 25% of their daily caloric ingestion reduced and E+C group had aerobic exercise and caloric restriction every other day. The results indicated that rats gained weight after 18 weeks of high-fat diet (P<0.05). Also, there was a significant difference in muscle DAG and serum levels of insulin, glucose and insulin resistance index between aerobic exercise, calorie restriction and combination groups and high-fat group (P<0.05). PKC-θ levels decreased in negative energy balance groups (P>0.05). Also, there was a significant difference between E and E+C groups and high-fat diet group in GLUT4 levels (P>0.05). This study indicated that negative energy balance methods can decrease skeletal muscle DAG even along with a high-fat diet which is largely congruent with an improvement in insulin resistance.

کلیدواژه‌ها [English]

  • aerobic exercise
  • calorie restriction
  • GLUT4
  • negative energy balance
  • Obesity

 

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