تأثیر تمرین استقامتی بر برخی پارامترهای متابولیکی موش‌های صحرایی دیابتی نوع دو ناشی از نیکوتین آمید و استروپتوتوزوسین

نوع مقاله: مقاله پژوهشی

نویسنده

استادیار، گروه فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه الزهرا (س)، تهران، ایران

چکیده

هدف از تحقیق حاضر، تعیین تأثیر تمرین استقامتی بر برخی پارامترهای متابولیکی موش­های صحرایی دیابتی نوع دو ناشی از نیکوتین آمید و استروپتوتوزوسین بود. بدین منظور، 24 سر موش نر بالغ غیرچاق پس از القای دیابت (تجویز نیکوتین آمید و استروپتوتوزوسین) به سه گروه هشت‌تایی شامل غیردیابتی، دیابتی تمرین و دیابتی کنترل تقسیم شدند. چهار هفته پس از پیشرفت دیابت، گروه دیابتی تمرین چهار هفته روی نوار گردان دویدند. در پایان تمرین، تست تحمل گلوکز درون‌صفاقی برای تعیین حساسیت به انسولین انجام گرفت، سطوح گلوکز و انسولین پلاسما اندازه­گیری و شاخص مقاومت به انسولین (HOMA-IR) محاسبه شد. از آزمون آماری تی- مستقل و آنالیز واریانس دوطرفه برای تحلیل داده­ها استفاده شد. براساس یافته­های تحقیق، در طول چهار هفته القای دیابت، بین وزن بدن، میزان غذای دریافتی و سطح انسولین پلاسمای ناشتای دو گروه دیابتی و غیردیابتی تفاوت غیرمعنا­داری مشاهده شد، ولی سطح گلوکز خون ناشتا و غیرناشتای گروه دیابتی نسبت به گروه غیردیابتی افزایش معنا­داری یافت. همچنین، پس از چهار هفته تمرین، بین وزن بدن و میزان غذای دریافتی سه گروه غیردیابتی، دیابتی تمرین و دیابتی کنترل تفاوت غیر معنا­داری مشاهده شد، ولی تمرین موجب کاهش معنا­دار سطح گلوکز پلاسمای ناشتا و HOMA-IR و افزایش معنا­دار انسولین پلاسمای ناشتا و تحمل گلوکز گروه دیابتی تمرین نسبت به گروه دیابتی کنترل شد. بنابراین، به‌نظر می­رسد چهار هفته تمرین استقامتی تأثیر مفیدی بر برخی پارامترهای متابولیکی موش­های صحرایی دیابتی نوع دو دارد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Endurance Training on Some Metabolic Parameters of Nicotinamide and Streptozotocin Induced Type 2 Diabetic Rats

نویسنده [English]

  • Fahimieh Kazemi
Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran
چکیده [English]

The aim of this study was to determine the effect of endurance training on some metabolic parameters of Nicotinamide and Streptozotocin induced type 2 diabetic rats. After the induction of diabetes (injection of Nicotinamide and Streptozotocin), 24 non-obese adult male rats were divided into 3 groups: non-diabetic (n=8), trained diabetic (n=8) and control diabetic (n=8). 4 weeks after the development of diabetes, the trained diabetic group ran on a treadmill for 4 weeks. At the end of the training, intraperitoneal glucose tolerance test was performed to determine insulin sensitivity. Plasma glucose and insulin levels were measured and HOMA-IR was calculated. Independent t test and two-way ANOVA were used to analyze data. Results indicated that during 4 weeks of diabetes induction, an insignificant difference was found in body weight, food intake and fasting plasma insulin level between diabetic and non-diabetic groups, but fasting and non-fasting blood glucose level significantly increased in diabetic group compared with non-diabetic group. After 4 weeks of training, an insignificant difference was found in body weight and food intake among non-diabetic, trained diabetic and control diabetic groups, but training significantly decreased fasting plasma glucose level and HOMA-IR and significantly increased fasting plasma insulin and glucose tolerance in trained diabetic group compared with control diabetic group. Therefore, it appears that 4 weeks of endurance training had beneficial effects on some metabolic parameters of type 2 diabetic rats.

کلیدواژه‌ها [English]

  • aerobic exercise
  • Glucose tolerance test
  • hyperglycemia
  • Nicotinamide
  • Streptozotocin
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