نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزشی عصب و عضله، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه شهید بهشتی، تهران، ایران
2 دانشیار،دانشکده تربیت بدنی و علوم ورزشی، دانشگاه شهید بهشتی، تهران، ایران
3 استادیار، دانشکده تربیت بدنی وعلوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران
4 استادیار، گروه تربیت بدنی و علوم ورزشی دانشکده ادبیات و علوم انسانی، دانشگاه ولی عصر رفستجان، رفسنجان، ایران
چکیده
مصرف مکمل‌های حاوی آنتی‌اکسیدان در بهبود عملکرد در دوران سالمندی اهمیت زیادی دارد. هدف این تحقیق، بررسی تأثیر یک دوره مکمل‌دهی عصارۀ دارچین بر پاسخ گیرندة نیکوتینی استیل‌کولین (nAChR)پس از یک جلسه فعالیت وامانده‌ساز در موش‌های صحرایی سالمند بود. 32 سر موش سالمند نژاد ویستار به‌طور تصادفی در چهار گروه ورزش وامانده‌ساز، دارچین، دارچین– ورزش و کنترل تقسیم شدند. موش‌ها در گروه دارچین و دارچین- ورزش mg/kg/day 200 عصارة دارچین به‌مدت 14 روز به‌صورت گاواژ دریافت کردند. در گروه ورزش و دارچین– ورزش، موش‌ها ابتدا با سرعت m/min 10 برای گرم کردن روی تردمیل راه رفتند و سپس هر دو دقیقه، m/min 2 به سرعت تردمیل اضافه شد تا سرعت به m/min 28 رسید. پس از بیهوشی، عضلۀ SOL و EDL در حالت استراحت، بلافاصله و چهار ساعت بعد خارج شد. از روش هموژن کردن و سپس وسترن بلات برای شناسایی تغییر متغیرها استفاده شدند. داده‌ها با استفاده از آزمون تی مستقل و تحلیل واریانس یکطرفه (One Way ANOVA) در سطح معناداری (05/0≥P) تجزیه‌وتحلیل شدند. نتایج پژوهش نشان داد که میزان nAChR بلافاصله و چهار ساعت بعد در عضلۀ SOL، در همۀ گروه‌ها به‌طور معناداری بیشتر از گروه کنترل بود (05/0˂ P). با وجود این در عضلۀ EDL این افزایش معنادار نبود (05/0≤P). به‌نظر می‌رسد که مکمل‌دهی عصارۀ دارچین و فعالیت استقامتی، روش مناسبی برای کاهش توسعة سارکوپنیا و افزایش سنتز nAChR در افراد سالمند باشد.
کلیدواژه‌ها
عنوان مقاله [English]
The Effect of Cinnamon Extract Supplementation on nAChR Response of Skeletal Muscle in Old Rats to Exhaustive Acute Exercise
نویسندگان [English]
- Fataneh Farahmand 1
- Maryam Nourshahi 2
- Mojtaba Salehpour 3
- Eman Fathi 4
1 PHD student in neuromuscular Exercise Physiology, Faculty of Physical Education and Sports Science, Shahid Beheshti University, Tehran, Iran
2 Associate Professor, Faculty of Physical Education and Sports Science, Shahid Beheshti University, Tehran, Iran
3 Assistant Professor, Faculty of Physical Education and Sports Science, Teacher Training Shahid Rajai, University, Tehran, Iran
4 Assistant Professor,Department of Physical Education and Sports Science Faculty of Literature and Humanities,Vali-E-Asar University, Rafsanjan, Iran
چکیده [English]
Supplements containing antioxidants play an important role in improving performance in the elderly. The aim of this study was to investigate the effect of cinnamon extract supplementation on nicotine acetylcholine receptor (nAChR) after one session of exhaustive exercise in old rats. 32 aged Wistar rats were randomly divided into four groups: exhaustive exercise, cinnamon extract, cinnamon extract and exhaustive exercise and control. Cinnamon extract and cinnamon extract and exhaustive exercise groups received 200 mg/kg/day of cinnamon extract by intra gastric intubation for 14 days. Exhaustive exercise and cinnamon extract and exhaustive exercise groups firstly ran on a treadmill at the speed of 10 m/min. for warm-up. Then, speed increased by 2 m/min. every 2 minutes to reach 28 m/min. The rats were anesthetized and their SOL and EDL muscles were removed at rest, immediately and after 4 hours. The homogenized and western blot methods were used to identify the changes in variables. Data were analyzed by independent t test and one-way analysis of variance (ANOVA) at the significance level (P≤0.05). Results showed that nAChR was significantly higher immediately and after 4 hours in SOL muscle in all groups than the control group (P<0.05). However, this increase was not significant in EDL muscle (P≥0.05). It seems that cinnamon extract supplementation and endurance exercise might be an appropriate approach to decrease the development of sarcopenia and to increase nAChR synthesis in the elderly.
کلیدواژه‌ها [English]
- Aging
- exhaustive exercise
- nicotinic acetylcholine receptor
- sarcopenia
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