تأثیر فعالیت ورزشی استقامتی بر بیان ژن SYD در نورون‌های حرکتی رت‌های مبتلا به نوروپاتی دیابت

نوع مقاله: مقاله پژوهشی

نویسندگان

1 باشگاه پژوهشگران جوان، دانشگاه آزاد اسلامی واحد بروجرد، بروجرد، ایران

2 دانشیار گروه فیزیولوژی ورزش دانشگاه تربیت مدرس، تهران، ایران.

3 استادیار گروه فیزیولوژی ورزش دانشگاه آزاد اسلامی واحد بروجرد، بروجرد، ایران.

4 استادیار گروه تربیت بدنی دانشگاه لرستان، خرم آباد، ایران.

چکیده

 
هدف از پژوهش حاضر بررسی تأثیر فعالیت ورزشی استقامتی بر بیان ژن SYD در نورون‌های حرکتی رت‌های مبتلا به نوروپاتی دیابت بود. بدین منظور، دوازده سر رت صحرایی بالغ نر نژاد ویستار به‌طور تصادفی در سه گروه چهارتایی دیابتی تمرین‌کرده (DT)، دیابتی تمرین‌نکرده (DC) و کنترل سالم (HC) قرار گرفتند. دو هفته پس از تزریق STZ برای القای دیابت، با اثبات نروپاتی دیابت توسط آزمون‌های رفتاری، پروتکل تمرین استقامتی اجرا شد. سپس نورون‌های حرکتی L4-L6 بافت نخاع استخراج، و بیان ژنSYD به روش Real time-PCR بررسی شد. نتایج نشان داد میانگین بیان ژن SYD در گروه DCنسبت به گروه HC به‌طور معنا‌داری بالاتر بود. بیان ژن SYD در گروه DT نسبت به گروه DCنیز به‌طور معنا‌داری کمتر بود. به‌طور کلی می‌توان نتیجه‌ گرفت در نورون‌های حرکتی رت‌های دیابتی، تنظیم افزایشیmRNA SYD  در پیام‌رسانی آسیب نورونی درگیر است و ورزش به‌عنوان یک راهبرد غیردارویی، می‌تواند آن را تعدیل و به سطوح نرمال نزدیک کند. 

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Endurance Training on SYD Gene Expression in Motor Neurons of Rats with Diabetic Neuropathy

نویسندگان [English]

  • maryam fouladvand 1
  • Reza Gharakhanlou 2
  • Ahmad Hematfar 3
  • Masoud Rahmati 4
2 Associate Professor, Department of Exercise Physiology, Tarbiat Modares University, Tehran, Iran
3 Assistant Professor, Department of Exercise Physiology, Islamic Azad University, Borujerd Branch, Borujerd, Iran
4 Assistant Professor, Department of Physical Education, Lorestan University, Khoramabad, Iran
چکیده [English]

 
The aim of the present study was to investigate the effect of endurance training on SYD gene expression in motor neurons of rats with diabetic neuropathy. 12 Wistar male rats were randomly assigned to 3 groups (each group 4 subjects): diabetic trained (DT), diabetic untrained (DC) and healthy control (HC). Two weeks after STZ injection to induce diabetes, diabetic neuropathy was demonstrated by behavioral tests and endurance training protocol was performed. Then, L4-L6 motor neurons of spinal cord tissue were removed, and SYD gene expression was analyzed with Real time-PCR methods. The findings showed that mean SYD gene expression in DC group was significantly higher than HC group. Also, SYD gene expression in DT group was significantly lower than DC group. Generally, it can be concluded that in motor neurons of diabetic rats, mRNA SYD up-regulation is involved in neuronal injury signaling and training as a non-pharmacotherapy strategy can modulate it and get it close to normal levels.

کلیدواژه‌ها [English]

  • diabetic neuropathy
  • Endurance training
  • gene expression
  • SYD
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