تأثیر تمرین هوازی در هوای آلوده به ذرات کربن سیاه بر بیان ژن TLR4 و TNF-α بافت ریۀ موش‌های صحرایی نر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه شهید بهشتی، تهران، ایران

2 دانشیار فیزیولوژی ورزشی گروه تربیت بدنی و علوم ورزشی دانشگاه تربیت مدرس، تهران، ایران

3 دانشیار مهندسی بهداشت حرفه‌ای، گروه بهداشت حرفه‌ای و محیط، دانشکدۀ علوم پزشکی، دانشگاه تربیت مدرس، تهران، ایران

4 دکتری فیزیولوژی ورزشی گروه تربیت بدنی و علوم ورزشی دانشگاه تربیت مدرس، تهران، ایران

چکیده

هدف از پژوهش حاضر بررسی تأثیر تمرین هوازی در هوای آلوده به ذرات کربن سیاه بر بیان ژن TLR4 و TNF-α در موش‌های صحرایی نر است. به این منظور 24 سر موش صحرایی نر از نژاد ویستار با میانگین تودۀ بدنی 97/26±29/279 گرم به‌طور تصادفی در چهار گروه الف) کنترل، ب) تمرین هوازی، ج) آلودگی و د) آلودگی+ تمرین قرار گرفتند. از دستگاه تزریق ذرات و اتاقک (فالونک) به‌منظور قرارگیری حیوانات در معرض ذرات کربن سیاه با قطر کمتر از 10 میکروناستفاده شد. پروتکل تمرین هوازی با 50 درصد بیشینۀ سرعت هر گروه به مدت 4 هفته انجام گرفت و 24 ساعت پس از آخرین جلسۀ تمرینی و قرارگیری در معرض ذرات کربن سیاه حیوانات قربانی شدند. با استفاده از تکنیک Real time-Pcr بیان ژن TLR4 و TNF-α در بافت ریۀ موش‌ها ارزیابی شد. به‌منظور تعیین معنادار بودن تفاوت بین گروه‌ها از تحلیل واریانس چندسویه و آزمون تعقیبی LSD و آزمون ناپارامتریک فریدمن استفاده شد. تمرین هوازی، به کاهش بیان ژن TLR4 و TNF-α در موش‌های در معرض ذرات کربن سیاه منجر شد. همچنین، تمرین با کاهش معنا‌دار تودۀ بدن در گروه‌های در معرض ذرات کربن سیاه همراه شد. چهار هفته تمرین هوازی در هوای آلوده به ذرات کربن سیاه به تعدیل عوامل التهابی TLR4 و TNF-α و تودۀ بدن منجر می‌شود. به‌نظر می‌رسد این تغییرات در بخشی با کاهش خطر ابتلا به بیماری‌های ریوی همراه باشد.
 
 
 

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Aerobic Exercise in Carbon Black Particulates Air Pollution on TLR4 and TNF-α Gene Expression in Lung Tissue of Male Rats

نویسندگان [English]

  • Mohammad Fashi 1
  • Hamid Agha-Alinejad 2
  • Hasan Asilian Mahabadi 3
  • batool rezaee seraji 4
  • behzad pak rad 4
1 Assistant Professor, Faculty of Physical Education and Sport Sciences, Shahid Beheshti University, Tehran, Iran
2 Associate Professor of Exercise Physiology, Department of Physical Education and Sports Sciences, Tarbiat Modares University, Tehran, Iran
3 Associate Professor of Occupational Health Engineering, Department of Environmental & Occupational Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4 PhD in Exercise Physiology, Department of Physical Education and Sport Sciences, Tarbiat Modares University, Tehran, Iran
چکیده [English]

The present study aimed at evaluating the effect of aerobic exercise in carbon black particulates air pollution on TLR4 and TNF-α gene expression in male rats. 24 male Wistar rats (mean body mass 279.29+26.97 gr) were randomly divided into 4 groups: A) control, B) aerobic exercise, C) pollution, D) exercise+pollution. A particulates injector and Falounak chamber were used to expose the rats to carbon black particulates with a diameter˂10 micron. Aerobic exercise protocol was performed in each group with 50% maximum speed for 4 weeks. The subjects were killed 24 hours after the last session and exposure to carbon black particulates. The gene expression of TLR4, and TNF-α were analyzed in the lung tissue by Real time – Pcr technique. In order to determine the significant differences among the groups, multiple ANOVA, LSD post hoc and non-parametric Friedman tests were used. Aerobic exercise reduced the gene expression of TLR4, and TNF-α in rats exposed to carbon black particulates. Also, the exercise significantly reduced body mass in those groups exposed to carbon black particulates. 4 weeks of aerobic exercise in carbon black particulates air pollution balanced inflammatory markers of TLR4, TNF-α and body mass. It seems that these changes are partly associated with a reduction in the risk of pulmonary diseases.
 
 

کلیدواژه‌ها [English]

  • aerobic exercise
  • carbon black
  • inflammation
  • lung tissue
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