تأثیر هشت هفته تمرین هوازی تناوبی بر بیان پروتئین ABCA1 لنفوسیتی، ApoA-I و پروفایل‌های چربی خون در نوجوانان پسر دچار اضافه‌وزن و چاق

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار گروه تربیت بدنی و علوم ورزشی، دانشکدۀ علوم تربیتی و روان‌شناسی، دانشگاه شهید مدنی آذربایجان

2 دانشیار گروه فیزیولوژی دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه تهران

3 استاد گروه فیزیولوژی دانشکدۀ تربیت بدنی و علوم ورزشی دانشگاه تهران

4 دانشیار بیوشیمی، مرکز تحقیقات سلولی و مولکولی، پژوهشکدة علوم غدد درون‌ریز، دانشگاه علوم پزشکی شهید بهشتی

5 استادیار گروه فیزیولوژی ورزشی، دانشکدۀ علوم انسانی، دانشگاه زنجان

چکیده

چاقی زودرس و انتقال آن به بزرگسالی، احتمال بروز بیماری CAD را افزایش می‌دهد. پروتئین ABCA1 نقش محوری در فرایند انتقال معکوس کلسترول و پیشگیری از  CAD دارد. هدف این پژوهش، بررسی تأثیر هشت هفته تمرین استقامتی تناوبی طناب‌زنی بر مقدار بیان پروتئین ABCA1 لنفوسیتی، ApoA-I و پروفایل‌‌های چربی خون در نوجوانان پسر دچار اضافه‌وزن و چاق بود. به این منظور 30 دانش‌‌آموز (میانگین سن 1/1±35/17 سال، وزن 6/11  ±6/88  کیلوگرم و شاخص تودۀ بدنی 36/2 ±31/28 کیلوگرم بر متر مربع) به‌صورت‌ تصادفی انتخاب شدند و در دو گروه کنترل (15n=) و تمرین (15n=) قرار گرفتند. پروتکل تمرین شامل تمرین استقامتی تناوبی طناب‌زنی (8 هفته، 4 روز،40 دقیقه در هر جلسه) بود. سنجش میزان بیان پروتئین ABCA1 لنفوستی از طریق همولیز سلولی و روش الایزای حساس انجام گرفت. داده‌ها به‌وسیلۀ آزمون آماری T-test در سطح معناداری 05/0>P تحلیل شد.یافته‌ها نشان داد میزان پروتئین ABCA1 لنفوسیتی (000/0=P) و VO2max (000/0=P) افزایش معنادار و مقادیر پلاسمایی TG (017/0=P)، TC (001/0=P)، LDL-c/HDL-c (026/0=P) و TC/HDL-c (002/0=P)، درصد چربی بدن (015/0=P) و BMI (042/0=P) کاهش معنادار داشتند. اما افزایش مقادیر Apo A-I،  HDL-c و کاهش  LDL-c و وزن بدن معنادار نبود. نتیجه اینکههشت هفته تمرین استقامتی تناوبی طناب‌زنی می‌تواند تأثیرات مثبتی بر پروتئین ABCA1 (به‌عنوان دروازه‌بان فرایند انتقال معکوس کلسترول) و پروفایل‌های چربی خون در نوجوانان با وزن زیاد و چاق داشته باشد.                                 

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Eight weeks Interval Endurance Training on Lymphocyte ABCA1 protein Expression , Plasma Apolipoprotein A-I and Lipid Profiles in Overweight and Obese Boy Adolescents

نویسندگان [English]

  • Bahloul Ghorbanian 1
  • Mohamad Reza Kordi 2
  • Ali Asghar Ravasi 3
  • Mehdi Hedayati 4
  • Agha Ali Ghasemnian 5
1 Assistant Professor, Physical Education and Sports Sciences Department, Faculty of Educational Sciences and Psychology, Azerbaijan Shahid Madani University, Tabriz, Iran
2 Associate Professor, Exercise Physiology Department, Physical Education and Sports Sciences Faculty, University of Tehran, Tehran, Iran
3 Professor, Exercise Physiology Department, Physical Education and Sports Sciences Faculty, University of Tehran, Tehran, Iran
4 Associate Professor of Biochemistry, Cellular-Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Assistant Professor, Department of Exercise Physiology, Faculty of Humanities, University of Zanjan, Zanjan, Iran
چکیده [English]

Abstract

Back grand: Early obesity and its transfer to the adult, increases likelihood incidence of coronary artery disease (CAD). ATP-binding cassette transporter) ABCA1) as a member of the ABC transporters family plays a crucial role in reverse cholesterol transport and CAD prevention.
Objective: The purpose of this study was to investigate ABCA1 expression in lymphocytes, plasma apolipoprotein A-I and lipid profiles in response to eight weeks interval aerobic endurance exercise as rope training.
Materials and Methods: Thirty students (17.3±1.1yr, 85.73± 11.68 kg and 28.41± 2.36 kg / m²) volunteered and randomly were assigned into training (n = 15) and control (n =15) groups. Exercise protocol was endurance rope training (8 wk, 4 d/wk and 40 min/d). cell hemolysis and sensitive Elisa method was used for Lymphocyte ABAC1 protein expression.T-student test was employed and data were analyzed by SPSS software (version 16).
Results: The independent-samples T-Test results were showed that after 8 weeks interval rope endurance training, the levels of lymphocyte ABCA1 expression(p=0/000) and VO2max(p=0/000) were significantly increased and plasma levels of TG(p=0.017), TC(p=0.001), LDL-c/HDL-c(0.026),TC/HDL-c(p=0.002) and measures of BF%(p=0/015)and BMI(p=0.042) as anthropometric indicators were decreased. Changes of other variables such increasing in Apo A-I , HDL-c, LDL-c and decreasing in body weight, were not significant. Significant level (p>0.05) was considered.
Conclusion: The findings of this study proved that 8-week interval aerobic endurance exercise as rope training can be have positive effects on lymphocyte ABCA1 protein expression(as goalkeeper of reverse cholesterol process) and lipid profiles and ultimately in prevention of arteriosclerosis among overweight and obesity adolescents.

کلیدواژه‌ها [English]

  • HDL-C
  • rope training
  • Adolescents
  • ABCA1
  • ApoA-I
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